A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a... | Contact experts in DNA to get answers
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Questions related to DNA
I am working on developing drive cycles of a particular city. I have collected raw driving data and processed the data to design a representative drive cycle. I would like to have a comparative...
16 April 2021 2,138 3 View
A limited number of genes are found in mitochondria beside nucleus. What's the difference between the genes found in mitochondria and nucleus.
13 April 2021 4,875 3 View
I'm working on a project to incorporate Casein into a aqueous solution that is required to remain shelf stable for up to a year. Our existing method utilized for other similar products uses both...
12 April 2021 436 3 View
Good time of day, Is it possible to analyze the carbon isotope ratio using MS QTOF? Like for example, analysis of a CO2 sample? Thanks!
27 March 2021 3,204 1 View
I have plotted the probability density bar diagram for wind data. Then I have estimated the Weibull parameters in different methods and do the same again. But I can not understand why the sum of...
24 March 2021 765 5 View
In general, it is not possible to observe simultaneously all nonlinear effects described by the nonlinear polarizations, because usually only one nonlinear process is phase-matched. In nonlinear...
06 March 2021 5,170 2 View
Hello fellow researchers, I am currently involved in a project that involves conversion of biomass to bio-oil using liquefaction technique. My experiment is conducted in a high-pressure,...
20 February 2021 9,271 3 View
Hello everyone, Do you recommend storing DNA and plasma aliquots (samples) at -20°C or -80°C for a period of 5 to 7 months knowing that subsequent analysis will be NGS? Thank you.
18 February 2021 4,432 3 View
Hi, I ran SDS-PAGE followed by a wet transfer into a PVDF membrane, but I did not get a good transfer. For cell lysis, I used RIPA buffer. I used Fluorescent Compatible Sample Buffer (4X) and...
18 February 2021 5,458 3 View
Need to know soon, whether to go in for Transcriptome analysis or GBS based method to unravel the Earliness trait in hirsutum cotton? Considering the compromises in both methods, cost involved and...
06 February 2021 7,465 3 View
Hi All, I do have a single-stranded oligonucleotide of 100 - 150 bases synthesized and the sequence is not known. How would I proceed to know the sequence .
05 February 2021 5,797 3 View
Hi, I need to make contact angle measurements on PTFE, ideally with diiodomethane. As you may know, the product is quite toxic (for repiratory system mostly). But my drope shape analyser is not...
04 February 2021 1,790 1 View
Hi everyone, I perform a PCR + Restriction Digestion (single cut, same at both sides) over a metagenomic library. After that, I am trying to purifiy DNA fragments between 1-4kbp long from the...
25 January 2021 2,439 5 View
Hi all, I am interested in using hemodynamic based functional imaging tools (ultrasound, photoacoustic, optical coherence tomography, etc) to measure the activity of axon fiber groups in large...
15 January 2021 6,478 1 View
I had a sample of plasmid extracted from e.coli and wanted to check the amount of Host cell RNA impurity with a fluorescent dye. to do this, first of all, I treated the sample with DNase I. (NEB...
12 January 2021 2,793 3 View
I have synthesized iron oxide naoparticles via co precipitation but after drying the powder nanoparticles were not attracted to magnet i want to use it for MR imaging so i need superparamagetic...
03 January 2021 7,414 4 View
Given that the core enzyme tends to DNA in the cell, because of the electrostatic force. Why is there no such tendency between RNApolymerase 2 and RNA?
15 December 2020 2,857 5 View
I've been trying to get DNA from unknown bacteria to show up on an agarose gel. I used the QIAprep spin mini prep kit on some unknown bacteria, so I should have gotten plasmid DNA. Using a nano...
08 December 2020 8,691 10 View
I am usiing LAMP kit, specifically designed for liriomyza huidobrensis. I have a random samples of differet insects and LAMP seems to amplify insects from other Genus as well. Although, other...
08 December 2020 5,791 3 View
Hello, I have been trying to ligate a 1.4kb insert in the pENTR D-TOPO plasmid using different molar ratios like 1:1, 2:1, and 3:1 (recommended by NEB) , but every time no colonies are seen. Only...
08 December 2020 3,169 5 View
My lab have some skin biopsies that were previously conserved in paraffine , and i wanted to know if it is possible to extract DNA or RNA from them.
07 December 2020 8,116 6 View
One of the steps during the preprocessing of the data from metatranscriptomic analysis is to remove any host reads (host contamination) by comparing to host database. But what if there is no host...
01 December 2020 3,491 2 View
Hi I'm interested in the production of insulin at a lab-scale. I read some papers about the topic. One of them mentioned autocleavage of the proinsulin without any enzymatic cleavage. But they...
28 November 2020 2,940 3 View
Hello! I'm using Ultra-Deep Microbiome Prep kit for the isolation of DNA from mice skin biopsies. I was doing it yesterday for the first time and I didn't achieve to any DNA yields. So I would...
20 November 2020 5,730 1 View