A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a... | Contact experts in DNA to get answers
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Questions related to DNA
I'm collecting data on dominant frequency for all tetrapods (birds, mammals, lepidosaurs, frogs, testudines, crocodiles) and I'm looking for big databases Thanks in advance!
18 May 2020 5,645 0 View
As we know STRING is a very good tool for the Protein-Protein interaction analysis. But we can analyze only 2000 protein. if it is more than 2000, is there any tool for PPI analysis.
20 April 2020 8,251 2 View
Hi guys, Am defining monocyte subsets. And classifying then to classical, intermediate and non classical. But when i am gatting non classical the CD14 expression seems smear up negative. What i...
16 April 2020 3,874 0 View
Phosphoric acid, when free in solution, is a triprotic acid with pKa1 = 2.2, pKa2 = 7.2, and pKa3 = 12.4. But most of the phosphate groups in DNA and RNA molecules participate in phosphodiester...
24 March 2020 5,066 15 View
Why a canonical AMBER simulation of DNA doesn't include dangling 5' phosphate? So, if I want to simulate an oligomer of length N bp, then it will have 2N bases but 2(N-1) phosphate when standard...
23 March 2020 6,255 3 View
I want to do NMR for my G-quadruplex forming Oligonucleotides in presence of a specific metal ion. Our lab is new to this topic and no one in our lab has experienced about this.I have tried using...
18 March 2020 8,456 2 View
Dear researcher, I am extracting DNA from IV plants which contain a lot of phenolic compounds and secondary metabolites, due to which the DNA extracted always contains impurities (I tried several...
06 March 2020 5,070 5 View
Hello, I'm currently working on DNA extraction from E. coli suspended in PBS. I'm working with concentrations ranging from 102 CFU/mL to 105 CFU/mL (and 108 CFU/mL as a positive control) and I...
03 March 2020 7,609 14 View
Hello everybody, --just a brief intro about the background of my question-- I have to analyse the gut microbiome of a marine fish through whole metagenome sequencing. Going through literature, I...
19 February 2020 8,548 1 View
Hi, I ran 100ng of gDNA on a 0.7% agarose gel at 80V for 45min in 1XTAE buffer which was made fresh from a 50X stock which was produced 3 days ago. The samples were loaded in wells 3 and 5 where...
18 February 2020 8,688 3 View
Dear colleagues, I hope for your help. We have two samples of DNA purified by different ways. The one (black) has a 260/280 ratio equaled 1,935, its 260/230 ratio is 2,488 For the second (blue)...
02 February 2020 8,249 3 View
I'm planning on substituting my media with a balanced mix of nucleosides. Any advice on concentrations or supplier?
15 January 2020 9,320 3 View
Hi everybody, I am struggling to get the TOP flash assay to work using 293T cells. I have tried adding 10 ug of recombinant Wnt3a as positive control and also using Wnt3a conditioned media but I...
15 January 2020 8,559 9 View
A mathematical model of temperature-dependent sex determination of crocodile eggs
10 January 2020 2,345 7 View
Usually after conducting Methylation specific PCR in order to validate the methylation status, one should sequence to see which cytosines are methylated.However if one does not have sequencing...
06 January 2020 6,756 4 View
01 January 2020 3,129 3 View
I'm using no template control (water + reagents) in my reaction in qPCR. IS it normal to have also in the no template control sample fluorescence detected? Normally i have no template plots...
01 January 2020 2,451 3 View
I use 2% Agarose gel with 90V and 60 minutes for my PCR product. I'm confused why my 100bp DNA ladder not separates well in SYBR Green but separate well enough when I'm using SYBR Safe with the...
18 December 2019 9,423 3 View
ΔG = ΔG°+ RT ln K How to derive this equation ? I searched it a lot but couldn't find a clear explanation.
15 December 2019 9,392 5 View
After isolating T cell gDNA using DNAzol and resuspending in NaOH and HEPES, I quantified DNA recovery using a Qubit and my yield was only about 50%. I think I overdried the pellet and the DNA...
12 December 2019 3,595 3 View
I still get Non-specific products & multi-peak Melt Curves despite changing the annealing temperature of my designed primers several times (58C, 59C, 60C, 61C, 62C, 63.7C & 65C). I've also...
12 December 2019 3,161 6 View
Hello Dears, i know the purpose of isopropenol in DNA extraction, collect the DNA in the form of pellet from the sample. what is the reason and logic we use chilled isopropenol in the extraction...
09 December 2019 9,224 6 View
Hello, For my research I am trying to figure out how to statistically compare Jacobs indices. I have scat data of four carnivores and an extensive diet analysis (Per scat what hair they found...
04 December 2019 1,687 0 View
Hi there! I used Agilent's Seahorse - Mito Fuel Flex kit for few experiments. Wave software is quite useful to analyze the data. It gives dependency, capacity and standard deviation values in...
25 November 2019 2,819 1 View