A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a... | Contact experts in DNA to get answers
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Questions related to DNA
Usually after conducting Methylation specific PCR in order to validate the methylation status, one should sequence to see which cytosines are methylated.However if one does not have sequencing...
06 January 2020 6,764 4 View
i am working on two fruit varieties and their differemt gene expression profile such as genes in volves in fruit cuticle wax biosynthesis. as according to references i have found some KCS,...
06 January 2020 4,377 6 View
I'm planning on substituting my media with a balanced mix of nucleosides. Any advice on concentrations or supplier?
01 January 2020 3,138 3 View
I'm using no template control (water + reagents) in my reaction in qPCR. IS it normal to have also in the no template control sample fluorescence detected? Normally i have no template plots...
01 January 2020 2,461 3 View
Recently I have synthesized a contrast agent for MRI ( Mn-Zn ferrite). And Now I want to take a NMR test. I know adding a solvent to it. But I dont know whether I must add water to it or no
27 December 2019 8,650 2 View
Hello, I need to verify results of IEEE 13 node with induction generator type of Distributed generation through steady state modeling PQ (V) type. Thanks!
24 December 2019 6,506 3 View
I use 2% Agarose gel with 90V and 60 minutes for my PCR product. I'm confused why my 100bp DNA ladder not separates well in SYBR Green but separate well enough when I'm using SYBR Safe with the...
18 December 2019 9,441 3 View
Upon reading about the treatments available for the incidence of HIV, I was thinking about if a similar approach to treatment can be developed and implemented for the treatment of Multiple...
18 December 2019 8,088 0 View
ΔG = ΔG°+ RT ln K How to derive this equation ? I searched it a lot but couldn't find a clear explanation.
15 December 2019 9,407 5 View
After isolating T cell gDNA using DNAzol and resuspending in NaOH and HEPES, I quantified DNA recovery using a Qubit and my yield was only about 50%. I think I overdried the pellet and the DNA...
12 December 2019 3,606 3 View
I still get Non-specific products & multi-peak Melt Curves despite changing the annealing temperature of my designed primers several times (58C, 59C, 60C, 61C, 62C, 63.7C & 65C). I've also...
12 December 2019 3,171 6 View
Hello Dears, i know the purpose of isopropenol in DNA extraction, collect the DNA in the form of pellet from the sample. what is the reason and logic we use chilled isopropenol in the extraction...
09 December 2019 9,241 6 View
Hello, For my research I am trying to figure out how to statistically compare Jacobs indices. I have scat data of four carnivores and an extensive diet analysis (Per scat what hair they found...
04 December 2019 1,694 0 View
Hi there! I used Agilent's Seahorse - Mito Fuel Flex kit for few experiments. Wave software is quite useful to analyze the data. It gives dependency, capacity and standard deviation values in...
25 November 2019 2,830 1 View
I cloned my designed gene fragment in L4440 vector under the control of two T7-promoters to get a double stradouble-strandednded RNA (dsRNA). This cloned vector is transformed into an HT115...
25 November 2019 7,071 1 View
We made adoptive transference from macrophages of C57BL/6 to Balb/c mice. Now we need to know if those cell migrated to local lesion in the paws and we thought to make a PCR with the DNA extracted...
24 November 2019 7,595 0 View
I am currently working on u937 human monocytes and have tried staining them with cd68 but I have not had any immunofluorescence signal. could anyone give any guidance please? is there another...
22 November 2019 9,545 2 View
May Magnetic resonance imaging test have drawbacks for who is working with iron oxide magnetic nanoparticles? I mean iron oxide nanoparticles are relatively non-toxic, but i'm not sure if i...
21 November 2019 5,532 3 View
I've transformed high-copy plasmid (pGEMT Easy - 13 Kbp) in E.coli (JM109) and did plasmid extraction with commercial kit from Macherey Nagel GigaPrep EF. However, the resulted concentration of...
11 November 2019 3,098 8 View
Hi everybody, I am currently working on my Bachelor Thesis, for which I am performing EMSA on CRISPR/Cas9. I am trying to bind Cas to a specific target, but every time I try I get these multiple...
11 November 2019 3,585 3 View
Hi everyone, I am performing ChIP-seq on a transcription co-factor, for that I had to first crosslink protein-protein with EGS (1.5 mM) for 25 min and then I added 1% formaldehyde for 10 min,...
11 November 2019 7,592 2 View
Atropine is an antagonist of the muscarinic acetylcholine receptors located in end organs (e.g. heart) as well as in brain. While acetylcholine is released from the vagal efferent fibers,...
02 November 2019 2,744 1 View
Hi Everybody, I am aiming to specifically knock down transcript variants of my GOI. Can anyone recommend vendors that design transcript variant specific siRNA? Please only reply if you have...
11 October 2019 9,549 0 View
I am completing my Master's in Biology on small mammal research (Sorex shrews) in Washington State. I am looking for a reliable lab in the US to send tail clips/liver samples to, to sequence the...
10 October 2019 312 2 View