A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a... | Contact experts in DNA to get answers
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Questions related to DNA
I have isolated DNA from 382 plants without running them on gel . I thought i will run them together after one month of completed extraction i found a long band of smear on my gel for all of...
03 March 2019 8,119 10 View
Hello! today I purified my pcr Products with minelute purification kit. I eluted the dna at the last step using the buffer (10ul). According to the protocol. that gave me around 9/10ul of purified...
03 March 2019 7,239 3 View
I've read that the following formula: (G3+)(N1-7)(G3+)(N1-7)(G3+)(N1-7)(G3+) is used to algorithmically search for potential g-quadruplex motifs in various genomes. I'm wondering whether certain...
03 March 2019 6,899 2 View
Can you use e-gel ladders on normal 2% agarose gels?
03 March 2019 3,593 10 View
I'm interested in the entry mechanism of the Zika Virus into U937 Monocytes. To the best of my knowledge, the proteins responsible for Zika entry are DC-SIGN, AXL, Tyro3, Mer, TIM and...
18 February 2019 8,489 3 View
What is considered the normal range (in mm Hg) for CSF opening pressure in a lumbar puncture? How serious is CSF opening pressure of 19 mm Hg?
11 February 2019 5,395 7 View
I am interested to know the reactivity of -NH2 groups of nucleobases (e.g. adenine, guanine, cytosine etc) in diazotization reactions. Any relevant reading suggestions are also much...
02 February 2019 9,773 4 View
Some researchers says that "The DNA sequences of the dark genes are very rich in G and C molecules, two of the four bases molecules that make DNA. We need to fully understand the concept of dark...
02 February 2019 4,922 6 View
How to distinguish Donor-specific Cell-free DNA from recipient Cell-free DNA when we have VCF file after performing next-generation sequencing.
02 February 2019 7,523 6 View
How can to be constant knowledge of what is new in my field so as not to lose my ability to keep abreast of the new application?
02 February 2019 7,629 9 View
I use PCR cleanup kit from Thermoscientific. The extraction of restricted plasmid from gel failed twice but the extraction of insert which was run parallely gave me DNA with good concentration and...
02 February 2019 2,055 18 View
I am not being unable to isolate DNA from J2 stage nematodes. The nematodes have been extracted from gall and currently are in water. I used extraction methods using sonnicator, glass beads,...
02 February 2019 4,623 6 View
hi, i am isolating DNA from 384 lines in a mapping population , but it takes my entire day for just 15 or maximum 30 samples . I want to do more than 30 in one time . Currently i am using 2...
02 February 2019 696 5 View
If I have part of the DNA, of a tyrannosaur and complete it with the DNA found from another tyrannosaur, can we be able to clone it on the mother cells of a Crocodile?
09 January 2019 1,412 8 View
Does anyone guide me the articles or research for the population estimates of crocodiles through using camera traps data? Any guidance would be greatly appreciated!
09 January 2019 4,396 6 View
Should I add oligos based on the chart flanking both the ends of the restriction site or adding oligos to the end of DNA is enough?
01 January 2019 2,726 6 View
I am trying to remove RNA contamination from my DNA samples. I gave RNAse treatment for DNA resuspended in 50 ul of TE buffer. After 1 hour of treatment I reprecipitated the DNA using 1/10 V of...
01 January 2019 2,608 4 View
Aptamers are single-stranded DNA or RNA that bind to their targets with high specificity and affinity. There are many tools to predict the 2D structures of ssDNA aptamers but I couldn't find a...
01 January 2019 5,096 5 View
For example, when RNA is isolated from a cell, how do these molecules (DNA,RNA,protein) pass through the pores?
01 January 2019 1,582 0 View
I have extracted DNA from Cacao leaf tissue using CTAB, chloroform isoamyl alcohol method. When I quantified my samples on Nandodrop they show a conc. of 300-800 (ng/ul) in 50 ul of TE buffer. The...
01 January 2019 9,690 15 View
We are attempting to extract DNA from Crotalus adamanteus via PCI extraction. After spec-ing on nanodrop, we noticed the neonate samples had significantly lower DNA concentrations compared to the...
01 January 2019 10,005 5 View
Does the hybridization percentage matter while measuring the melting temperature of short DNA at the spectrophotometer? If it is the case, at low hybridization percentages, I suposse it is more...
01 January 2019 3,044 3 View
Which type of PCR techniques is the best in your opinion? There are several different types of PCR techniques. Any technique is used as the best for most reliable, accurate diagnosis, fast, and...
01 January 2019 1,128 21 View
Is a skin biopsy, stored in saline, refrigerated one month at +4 °C, still expected to be suitable for a bacterial PCR-DNA analysis? How fast does the DNA break down, and does it depend on which...
01 January 2019 3,624 5 View