A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a... | Contact experts in DNA to get answers
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Questions related to DNA
I isolated mRNA from HEK293T cells using oligo dT cellulose, followed by RNA Cleanup and Concentrate kit that includes a DNAse digest. The final mRNA yield was about 6.7ug, with 260/280 and...
26 August 2021 1,619 5 View
We are trying to spin coat PDMS on a Si substrate to achieve PDMS thickness of less than 5 microns.
24 July 2021 4,206 4 View
Hey, I try ti explain as best as possible, while being relatively new to this field. I want to perform a qPCR with the mcrA gene sequence (0.5kbp roughly) as standard. Ergo I want to quantify...
23 July 2021 539 0 View
I'm working with very small samples and I'm wondering if I should include the on column DNAse step or leave it out. I know the columns should do a good job even without this, and I've found a few...
16 June 2021 454 3 View
I was wondering whether it is possible to turn off the expression of a transcription factor that is expressed by CD8 T cells, and if yes, how?
04 June 2021 8,198 1 View
I'm trying to get anhydrous formamide for dissolving inorganic salts(LiH, NaNH2) in the glove box, but it is hard to find commercial anhydrous formamide. Thus I'm looking for how to drying...
04 June 2021 4,448 2 View
I have been using electrochemical techniques recently for a project and I have observed that the concentration of ferricyanide is always taken more than ferrocyanide. Also, oxidation potential is...
24 May 2021 7,658 9 View
Hi all, I am posting our paper on medrxiv. I have never come across Distribution/Reuse Options to choose from and those look copyright-related. It reads: © No reuse/adaptation without...
20 May 2021 4,543 4 View
During the energy minimization of a protein before Molecular Dynamic Simulation in GROMACS software, I am getting this error: "One or more water molecules can not be settled in GROMACS". Just...
18 May 2021 4,405 10 View
I am working with mouse primary cortical neurons that are plated in a 96-well plate. I treat the cells with a ranging drug concentration for 24 h and then measure LDH using a kit to determine...
18 May 2021 707 3 View
waste water treatment
14 May 2021 6,769 9 View
Hello, It's been a while since I've come across this idea of abortive rnas having a function other than instant repression, or at least, 'function' regarding the initiation of transcription....
05 May 2021 3,566 4 View
A limited number of genes are found in mitochondria beside nucleus. What's the difference between the genes found in mitochondria and nucleus.
13 April 2021 4,861 3 View
I'm working on a project to incorporate Casein into a aqueous solution that is required to remain shelf stable for up to a year. Our existing method utilized for other similar products uses both...
12 April 2021 426 3 View
Good time of day, Is it possible to analyze the carbon isotope ratio using MS QTOF? Like for example, analysis of a CO2 sample? Thanks!
27 March 2021 3,191 1 View
Hello fellow researchers, I am currently involved in a project that involves conversion of biomass to bio-oil using liquefaction technique. My experiment is conducted in a high-pressure,...
20 February 2021 9,261 3 View
Hello everyone, Do you recommend storing DNA and plasma aliquots (samples) at -20°C or -80°C for a period of 5 to 7 months knowing that subsequent analysis will be NGS? Thank you.
18 February 2021 4,396 3 View
Hi, I ran SDS-PAGE followed by a wet transfer into a PVDF membrane, but I did not get a good transfer. For cell lysis, I used RIPA buffer. I used Fluorescent Compatible Sample Buffer (4X) and...
18 February 2021 5,442 3 View
Need to know soon, whether to go in for Transcriptome analysis or GBS based method to unravel the Earliness trait in hirsutum cotton? Considering the compromises in both methods, cost involved and...
06 February 2021 7,453 3 View
Hi All, I do have a single-stranded oligonucleotide of 100 - 150 bases synthesized and the sequence is not known. How would I proceed to know the sequence .
05 February 2021 5,767 3 View
Hi everyone, I perform a PCR + Restriction Digestion (single cut, same at both sides) over a metagenomic library. After that, I am trying to purifiy DNA fragments between 1-4kbp long from the...
25 January 2021 2,404 5 View
Hi all, I am interested in using hemodynamic based functional imaging tools (ultrasound, photoacoustic, optical coherence tomography, etc) to measure the activity of axon fiber groups in large...
15 January 2021 6,460 1 View
I had a sample of plasmid extracted from e.coli and wanted to check the amount of Host cell RNA impurity with a fluorescent dye. to do this, first of all, I treated the sample with DNase I. (NEB...
12 January 2021 2,763 3 View
Given that the core enzyme tends to DNA in the cell, because of the electrostatic force. Why is there no such tendency between RNApolymerase 2 and RNA?
15 December 2020 2,825 5 View