A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a... | Contact experts in DNA to get answers
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Questions related to DNA
I require the empty vector of this plasmid: https://www.addgene.org/40346/ The ends have been destroyed: 5′ cloning site XhoI/SalI (destroyed during cloning) 3′ cloning site SalI/XhoI...
13 December 2023 1,251 4 View
Which climate threats and climate changes are more obvious in Europe, Asia and Iran? Question Parts of Eastern and Southern Europe and Asia, in particular, have experienced heavy rains that have...
23 November 2023 5,396 0 View
Hi, i am working on 13 node feeder DIgSILENT powerfactory with renewble resources like pv and wind turbine. If anyone work on this please can you share pfd file. its very helpful for me in my...
22 November 2023 4,417 0 View
Hi All, I recently differentiated U937 with PMA and IL4 to M2-macrophage. And stain it with CD206 PE (from biolegend) and CD68 (from BD). When it stained 10ul in fresh, there is positive peak. But...
16 November 2023 1,337 0 View
Hello, We are currently undertaking research to determine whether bacteria that are capable of lysing red blood cells will go on to produce biofilms. There are conflicting findings regarding this...
04 October 2023 4,741 2 View
Could you recommend a ointment base for adding potential active compounds and evaluate the benefits for wound healing? A database for ointment formulations? A basic procedure to prepare elemental...
03 October 2023 6,372 0 View
Hello I live in Iran and this kind of kits are not available for us here, Could someone tell me how can I find if?
26 September 2023 4,807 1 View
a lot of boreholes dung in the area hardly survive throughout the year. during dry season, one hardly finds water in the borehole.
30 August 2023 3,643 0 View
Does anyone have access to the following (quite old) article: A sample size correction for Pearson Chi-Square in 2 × 2 con... It would seems that I have no institutional access to it, and i was...
30 August 2023 6,020 1 View
What are the differentiating factors between PyTorch and TensorFlow, two major Python libraries for Machine Learning, in terms of flexibility, ease of use, and industry adoption?
11 August 2023 4,800 4 View
What is the effect of predation on prey in an ecosystem and relationship between a predator and prey in a predation relationship?
17 July 2023 2,724 0 View
I got DNA as 500 to 2300 ng/ul with 1.5 260/280 ratio but still the color of the DNA is brown , how to remove this brown color?? I need this DNA for NGS analysis whether this is good for NGS...
12 July 2023 7,988 2 View
Please tell us process of protein aggregation.
10 July 2023 2,795 1 View
There is difference between plagiarism softwares employed by journals and the free websites that are available online. which website is closest to professional plagiarism checker softwares ? when...
07 July 2023 1,867 1 View
I have diluted an E.coli sample of 1*10^8 CFU/mL to 1 CFU/mL by serial dilution process. Now, I have a doubt that how many E. coli bacteria will be present in that 1 CFU/mL solution. It would be...
05 July 2023 9,637 0 View
If someone have information or papers regarding this, I will appreciate.
27 June 2023 3,665 0 View
I have some clinical samples of human synovial fluid and I am extracting the bacterial DNA. I was wondering it is possible to spin the samples to separate the bacterial cells from the human cells...
14 June 2023 517 4 View
Our gel with too combs is too big for the imager so we cut it into four parts. Now only one piece has a positive and negative control (but the run and electrophoresis are the same). Can I crop the...
08 June 2023 9,099 1 View
During the sample preparation of cell lysate, add cell lysis buffer, agitate the cells, centrifuge, and recover and aliquot supernatant. What is the procedure/protocol for cell pellets preparation...
07 June 2023 5,362 3 View
I’m working on recombinant protein expression. My bacteria lysis protocol: The grown cells (Bl21) were harvested by centrifugation at 5000g for 20 min at 4 ◦C, resuspended in 5mL of PBS buffer,...
31 May 2023 1,385 3 View
Greetings, I am currently a graduate student taking Introduction to Research and Evaluation in Education. I've been tasked with posing the question of, "How does one define Research vs...
20 May 2023 5,013 2 View
For example why Tricine - Tris gives better resolution than Glycine - Tris in this papper? https://www.nature.com/articles/nprot.2006.4 I know it is related to the ion fronts but I don't truly...
19 May 2023 1,316 3 View
Hi all. Do you know the STR profile of SK-MEL-103 melanoma cell line? I guess it is not available from ATCC... Thank you.
17 May 2023 3,982 1 View
How can I add the number at risk below KM curves in GraphPad Prism? Thank you
12 May 2023 9,120 1 View