I am preparing CTAB buffer and I read that addition of PVP (Polyvinylpyrrolidone) helps to remove phenolic compounds. Most of the times in protocols there is no additional information about average molecular weight of used PVP, but I found in some, where it was specified that it was PVP 40 (mol wt 40 000) for DNA extraction. But I wanted to know if I can use the same for RNA extraction or should I use PVP 360 (mol wt 360 000)?
PVP is basically a polymer, which will only mention as %. So its better to go with dilution. In my experience, CTAB-buffer with 2% PVP works well for the Isolation of DNA. Incubate your CTAB with PVP for 15 mins at 65 C and then vortex.
PVP is basically a polymer, which will only mention as %. So its better to go with dilution. In my experience, CTAB-buffer with 2% PVP works well for the Isolation of DNA. Incubate your CTAB with PVP for 15 mins at 65 C and then vortex.
I think it is better to use the recommended concentration or dilution.
- These sorts of compounds are non specific in action
- That said it is better to use the correct concentration and molecular weight otherwise you might experience non specific issues with things like viscosity
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