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Questions related to DNA Extraction
I am currently facing difficulty in the DNA extraction because of the viscosity of the sputum samples. I am using the Chelex based extraction method which does yield in DNA but it is inefficient...
12 December 2019 3,231 1 View
I am developing a phylogeography workflow and I need PERMUT cpSSR to compute NST and GST for mitochondrial Cytochrome c Oxidase (COI) haplotypes so that I can test for genetic differentiation...
09 December 2019 690 2 View
The blood I am using has been collected from Nile Crocodiles and is stored at -20°C.
21 November 2019 1,095 3 View
I extract DNA from various sludge samples according to the method by Griffiths et.al (2000). By now I did not have any problem, even in case of sludges which contain a great amount of silage and...
15 November 2019 2,577 3 View
Hello, We recently obtained some insect samples (Asphondylia) collected a few months ago by collaborators. The individuals were freeze dried, placed into pure ethanol, and shipped to us on dry...
11 November 2019 1,171 2 View
11 November 2019 8,312 3 View
11 November 2019 1,498 3 View
I'm looking into extracting DNA from buccal cells. I have no experience in using this source material and was wondering if there was a general consensus on preferred method? Yield would be a...
11 November 2019 9,205 3 View
Are there any significant issues with using standard plastic falcon tubes for DNA extraction with chloroform? How long can chloroform be in contact with polypropylene for it to have a negative...
18 October 2019 3,816 4 View
Tried various protocols none is working. Generally getting DNA contaminated with phenols and proteins.
10 October 2019 7,852 5 View
10 October 2019 3,426 4 View
10 October 2019 8,663 4 View
I'm interested in fish genetics and phylogenogenetics. I might choose this topic for my PhD, however I have a concern of how samples are acquired for these kinds of studies. For fishes >8-10...
10 October 2019 417 3 View
Does anyone have a protocol or experience with extracting genomic DNA from very small sample volumes of 10-30 microliters? The sample is a microbial enrichment from a sewage treatment plant.
01 October 2019 7,024 1 View
Briefly, I need a cost and time effective method - which completely exludes the possibility of contamination between samples - in order to get ranaviral DNA from the liver of EtOH stored...
18 September 2019 8,437 6 View
This is my first time using commercialised PCI (25:24:1) solution. After adding equal amount of PCI into my supernatant, two of my samples did not form two layers as they should be. After...
09 September 2019 4,934 3 View
09 September 2019 5,583 7 View
04 September 2019 9,625 3 View
Iwill be so grateful if any researecher can share me protocol of DNA extraxtion using SDS/PK/CTAB method . thanks
30 August 2019 2,125 3 View
I used HotShot protocol to extract DNA from pig ears, needing to do PCR RFLP. Most of my samples I had successful PCR first time, and succesfsul RFLP. For some samples I only get very faint bands,...
27 August 2019 9,229 6 View
Dear all, I have used ATMAB protocol for pines DNA extraction. But, after incubation in a heat bath, the solution started black in colour. I applied washed with chloroform for carotenoids but not...
26 August 2019 5,650 3 View
I am researching two isoforms of an endolysosomal ion channel. I have found that when one is knocked down with siRNA, the expression of the other increases and vice versa. I cannot see this being...
21 August 2019 6,919 2 View
Hi there, I have been performing fmoc removal on my oligonucleotides using 10%piperidine. However, it seems that piperidine was found still left in solution after ethanol precipitation. Dos...
08 August 2019 9,761 3 View
08 August 2019 5,004 3 View