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Questions related to DNA Extraction
I've been measuring the gas emission of a lab-scale peatland experiment with a electrochemical sensor. The output of the sensor measures the concentration in ppm. I want to change this data to EF....
26 May 2021 6,965 1 View
If it is possible, suggest me a protocol, or suggest me a researcher or lab where this kind of research is active. Particularly, the main interest is cell division rate and cell morphology change...
10 May 2021 5,333 5 View
I am aiming to extract total DNA (eDNA) from samples containing cells (plant and animal), organelles (mitochondria and chloroplasts) and dissolved DNA. Using mechanical lysis (bead-beating) could...
05 May 2021 5,070 0 View
Hi everybody, can anybody suggest how to extract DNA from insect samples conserved in silica gel (in my case, mosquitoes)? Does anybody know a commericial kit which does that or a particular...
11 February 2021 3,676 4 View
- The Eppendorf tube was then placed in a microcentrifuge. Notice how it is balanced with another tube placed opposite it in the centrifuge drum to ensure smooth rotation. Other information...
25 January 2021 2,740 3 View
I would like to run molecular diversity indices using Tamura & Nei as a distance method for calculating nucleotide diversity of mtDNA control region sequence. It is difficult for me to...
20 January 2021 5,319 1 View
Hi there, I'm looking for a High Molecular Plant DNA Extraction Kit for subsequent Nanopore sequencing. Does anyone have experience using commercially available kits? Any...
04 January 2021 9,617 4 View
I know this is an elementary question, but I've been struggling to find an answer. RAxML calculates "pairwise ML distances" (with the option -f x), what does these distances mean? what are the...
23 December 2020 4,274 5 View
I am trying to know which are accelerating additive that will help in hardening of hydrated lime by addition of water in 1:1 ratio & after hardening it must be hard like stone (CaCO3) by...
28 October 2020 4,511 0 View
I want to separate DNA from human old clotted blood . I haven't any idea how to extract DNA from it. Your kind expertise and thought would be appreciated.
08 October 2020 8,040 2 View
Is it an effective method to isolate DNA from Gram-negative bacteria with only the use of a boil and freeze-thaw protocol, without any chemical purification? Also, is there a media to maintain the...
07 October 2020 7,101 8 View
I spiked in 4-8 log CFU/ml of Lactococcus lactis into milk, and use DNeasy Powered Food Kit to extract DNA from it. Nanodrop 2000 was used to determine the values. The yield and quality are weird:...
05 October 2020 6,342 4 View
Hi, Could anyone explain how Sciex OS Analytics software internally handles IS peak area calculations to account for Solid Phase Extraction recovery of a particular analyte in a targeted...
29 September 2020 3,414 1 View
We shall collect biopsies from skin/hoof of animals for bacteriological microbiome analysis. We need suggestions on DNA extraction kit that may be used for this. We prefer to keep the biopsies in...
25 September 2020 7,000 4 View
I will be extracting trypanosome DNA from mid-guts of tsetse flies for LAMP analysis. Kindly advice on protocol to adopt for maximum yield and benefits of Chelex method over the regular method....
24 September 2020 678 4 View
Hello, I've encountered a significant issue with Qubit 2.0 readings on DNA extracts of Arctic plant roots. The samples have all been run previously, and when double-checking them, the readings...
22 September 2020 6,491 0 View
Is there any way in which we can check whether the DNA inside the cell is negatively or positively supercoiled?
13 September 2020 8,992 2 View
Does anyone have experience of extracting DNA out of dairy cow's milk? I am interested in the DNA present in the B-cells. I have done extraction from blood but want to try extraction from milk. I...
03 September 2020 3,664 5 View
I have a large set of samples to extract DNA from, and am trying to plan out the most efficient way to process them. I have a few options, but the most efficienct would be to first process all of...
17 August 2020 3,017 9 View
I'm digesting a few different mammalian tissues with a spin kit and proteinase k. Some tissues (esp. testis and pituitary gland) look fully digested and clear. But once the lysis buffer is added,...
10 August 2020 4,565 2 View
Hi, I am trying to source a 96 well magnetic separation plate. Most of the examples I have found seem to have a limited number (24) of magnetic positions on a plate. Is there a reason there are...
10 August 2020 7,490 1 View
I'm extracting DNA from various bull tissues and there are some that don't work with a spin column kit, like brain tissue that contains a lot of lipids. For those, we do Phenol-chloroform...
06 August 2020 2,263 2 View
Hello, I want to extract DNA for ITS sequencing to identify unknown bacteria growing together with Dothistroma isolates. What kit may I use? Normally we use EZNA fungal and plant kits, it is...
31 July 2020 6,700 7 View
While running real.exe am getting the following error. Can anyone help me out. 01 2020-07-15_09:00:00 Turning off use of TROPOPAUSE level data in vertical interpolation Using sfcprs3 to compute...
17 July 2020 9,476 0 View