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Questions related to DNA Extraction
I noticed on the Internet and even on RG that many researchers escape the answer of this question, where they say it is not good or bad, but it is low and high. For low SDs, we know that the...
30 September 2021 2,627 7 View
How to prepare the input file for software PERMUT to calculate Gst and Nst. I have 14 haplotypes from 15 populations (6-8 individuals in each population) and i need to know the presence of...
15 September 2021 3,949 0 View
Hello. I'm doing the experiment about detect of MICA/B and ULBP families on cancer cell. The antibodies that i used is MICA/B(e-bioscience 320918, PE-Cy7) and ULBP2/5/6(rndsystems FAB1298P,...
22 July 2021 845 1 View
Hello, I am struggling with library preparation for Illumina sequencing. Currently I prepared 6 libraries, but only 2 of them were OK and ready for sequencing. The other 4 contained high amount...
09 July 2021 9,690 0 View
Hi everyone, My cols and I got the QIAamp Fast DNA Stool Mini Kit in order to get DNA from sea lion scats to analyze the diet of these animals. However, a colleague warned not using this "Fast"...
17 June 2021 6,347 0 View
Hello, I've been having an issue with my PCR amplicon appearing smaller on a gel than it should be. For reference, the band should be ~500bp, but it consistently runs closer to 250 bp according to...
13 June 2021 8,628 6 View
I am collect population differentiation from published papers. Sometimes, there are no statistics such as Gst, Nst, Ht, Hs and their SE. I will calculate Gst and Nst using PERMUT...
13 June 2021 3,910 0 View
the concentration of 10% HCl adequate to digest 10grams of fruit waste
08 June 2021 275 5 View
I have a concatenated file (fasta, nexus, or phylip) of ~550 loci. I need to convert this to a GENEPOP file format with different populations noted within it. Is there an easy way to accomplish...
27 May 2021 1,827 0 View
I've been measuring the gas emission of a lab-scale peatland experiment with a electrochemical sensor. The output of the sensor measures the concentration in ppm. I want to change this data to EF....
26 May 2021 7,008 1 View
Kindly assist to identify these two pathogens isolated from avocado and African cherry leaves respectively and cultured on Malt Extract Agar. It is slimy and has a very strong smell.
17 May 2021 752 7 View
If it is possible, suggest me a protocol, or suggest me a researcher or lab where this kind of research is active. Particularly, the main interest is cell division rate and cell morphology change...
10 May 2021 5,369 5 View
I am aiming to extract total DNA (eDNA) from samples containing cells (plant and animal), organelles (mitochondria and chloroplasts) and dissolved DNA. Using mechanical lysis (bead-beating) could...
05 May 2021 5,106 0 View
Hi everybody, can anybody suggest how to extract DNA from insect samples conserved in silica gel (in my case, mosquitoes)? Does anybody know a commericial kit which does that or a particular...
11 February 2021 3,857 4 View
I am running bacterial and Candidal MICs using antimicrobial peptides, some of these come in trifluoroacetate salt. I am using a radial diffusion assay technique using TSB agar. Previously, I have...
09 February 2021 238 0 View
- The Eppendorf tube was then placed in a microcentrifuge. Notice how it is balanced with another tube placed opposite it in the centrifuge drum to ensure smooth rotation. Other information...
25 January 2021 2,926 3 View
I would like to run molecular diversity indices using Tamura & Nei as a distance method for calculating nucleotide diversity of mtDNA control region sequence. It is difficult for me to...
20 January 2021 5,370 1 View
Hi there, I'm looking for a High Molecular Plant DNA Extraction Kit for subsequent Nanopore sequencing. Does anyone have experience using commercially available kits? Any...
04 January 2021 9,738 4 View
I know this is an elementary question, but I've been struggling to find an answer. RAxML calculates "pairwise ML distances" (with the option -f x), what does these distances mean? what are the...
23 December 2020 4,339 5 View
I am trying to know which are accelerating additive that will help in hardening of hydrated lime by addition of water in 1:1 ratio & after hardening it must be hard like stone (CaCO3) by...
28 October 2020 4,545 0 View
I want to separate DNA from human old clotted blood . I haven't any idea how to extract DNA from it. Your kind expertise and thought would be appreciated.
08 October 2020 8,148 2 View
Is it an effective method to isolate DNA from Gram-negative bacteria with only the use of a boil and freeze-thaw protocol, without any chemical purification? Also, is there a media to maintain the...
07 October 2020 7,222 8 View
I spiked in 4-8 log CFU/ml of Lactococcus lactis into milk, and use DNeasy Powered Food Kit to extract DNA from it. Nanodrop 2000 was used to determine the values. The yield and quality are weird:...
05 October 2020 6,446 4 View
Hi, Could anyone explain how Sciex OS Analytics software internally handles IS peak area calculations to account for Solid Phase Extraction recovery of a particular analyte in a targeted...
29 September 2020 3,485 1 View