Filter binding assay in reverse?-Detection of proteins that get immobilized by RNA binding?

Hey, I am characterizing a competitive inhibition of a host and viral RNA biding proteins. The RNA binding is not sequence specific but dependent on secondary structure of the RNA. I am looking...

08 January 2021 6,564 3 View

What is the name of a man that was able to memorize all the phone books in England?

I remember learning about this man in my neuroscience courses when studying learning and cognition. From what I can remember, he is/was British, on the spectrum, worked for either the post...

21 April 2020 1,133 3 View

DdRAD library preparation- double bands after PCR?

09 March 2020 3,304 1 View

Would whole-proteome iTRAQ analysis be an appropriate method for validating and quantifying silencing activity of artificial miRNAs?

I have used iTRAQ for analysis of the arabidopsis secretome, however, I am now hoping to apply this method to the whole proteome of various crop species. Advice on sample preparation or...

29 January 2020 9,994 3 View

How long can you store extracted xanthophyll samples?

Samples were extracted following Thayer and Bjorkmann 1990 using 80% cold acetone and were stored in a freezer in HPLC vials. Should I expect degradation of pigments with time?

11 November 2018 7,499 2 View

How can I collect blood from a neonatal mouse?

I would like to collect blood from a 1 day old mouse, terminal collection is fine because I also need to collect tissues. Can this be done by cardiac puncture or what method do people use to...

05 July 2018 4,326 16 View

Alternatives to ImageJ?

Are there more efficient techniques available, rather than ImageJ, for quantifying herbivory damage from photos of leaves? I have a basic understanding of machine learning but don't want to spend...

19 June 2018 3,021 3 View

Supplier for xanthophyll pigment standards?

I am trying to find a supplier in the US to purchase HPLC pigment standards for the xanthophyll cycle pigments, specifically antheraxanthin. I have found a supplier for zeaxanthin and...

31 October 2017 5,902 3 View

Has anyone used Dedoose qualitative analysis software? What did you think of it?

I'm a qualitative researcher not currently attached to a University and so without access to expensive, traditional QDA software. I'm looking for an affordable alternative that my employer won't...

12 October 2017 1,993 7 View

Is there a generally accepted substitution rate for fish mitochondrial DNA? Or, specifically, for ND2?

For divergence time estimation

15 March 2017 2,524 3 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

No title

25 February 2021 5,713 1 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

How to search for crystal structure for material with atomic position labelled?

I am using the crystallographic open database and materials project to search for cif file but the exact structure is not mentioned or how can I deduce that the given structure and atomic position...

23 February 2021 6,723 2 View