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Questions related to DNA Extraction
According to the following figure, we can know how lactic acid bacteria can make clear area. But I'm so confused.. because other bacteria also can make other acid besides lactic acid.. And I think...
07 January 2019 6,198 8 View
Can the moleculer weight of PEG (8000 and 6000) cause a noticeable effect in phage precipitation???
23 December 2018 645 3 View
I have been trying to validate a qPCR reaction, where the expected amplicon is 98 bp long. Initially, I made standard dilutions from qualitative PCR product. However, when I ran it the first few...
20 December 2018 4,361 9 View
12 December 2018 9,766 4 View
Our ultimate goal is to measure microRNA gene expression. We have tried several kits including Qiagen and all gave poor values for RNA concentration and 260/280. Any suggestions or ideas would be...
12 December 2018 3,678 6 View
Dear all, Are there any works that describe the positive relation between the diel vertical migration speed and the cells survivability for phytoplankton cells? Thanks you in advance Best...
08 December 2018 3,281 2 View
Dear Colleagues, I am looking for an adhesive film or filter that will decay when submersed in lysis buffers such as ATL (Qiagen). I once had a sample of such filters a couple of years ago, but I...
30 November 2018 4,162 2 View
So we use Ethidium bromide to stain our agarose gels for dna extraction and analysis. The software in our gel doc is malfunctioning so we are unable to capture images. So now we are using our...
27 November 2018 2,958 7 View
I am currently working on dna extraction from soybean leaves. Months prior, I could get clean and clear bands of DNA extracts when viewed in the agarose gel. But this time around, when I checked...
14 November 2018 4,002 8 View
11 November 2018 6,080 2 View
11 November 2018 9,275 7 View
I want to do DNA extraction from blood by following Protocol 6 Rapid Isolation of Mammalian DNA (Sambrook). One of the equipment is CH3COOK, but I have no CH3COOK in my laboratory and only...
11 November 2018 9,607 6 View
I'm already done quantifying the viral DNA using absolute quantification (qRTPCR). Can anyone teach me how to convert my data into copies/gram? Currently it is in copies/ul. Here are some...
11 November 2018 1,506 4 View
10 November 2018 3,600 3 View
Hi, I'm going to be using 0.1mm zirconia/silica beads to break apart my mycobacterial cells so I can extract DNA. Are there any tips for cleaning them? RPI says " beads can be soaked in a 1:10...
09 November 2018 6,359 1 View
04 November 2018 4,607 3 View
Looking expert opinion... I have collected marine sponge samples and were shadow dried for two weeks. Now the sponge samples are well dried and can be directly powdered by grinding. I would like...
10 October 2018 893 3 View
iwm looking for good protocol for dna extraction from nails clipping. does anyone can help? thanks, michal
10 October 2018 6,451 3 View
I'm attempting to extract DNA from a single retrix from a small sparrow. I have upwards of 200 samples but only one feather per individual. The feathers have no visible blood spots on them. I've...
15 August 2018 5,339 2 View
I would like to know if someone has used it and what performance you have obtained compared to similar kits. Is it easy and fast as the product description says?
08 August 2018 5,164 0 View
08 August 2018 4,671 2 View
I found cytoplasmic male sterility (CMS) occurs in a potato variety while never observed in another closely related variety. Now I already got the mitochondrial genome data (around 800K each) of...
06 August 2018 201 5 View
I want to know What´s the best method for microbial DNA extraction of Theobroma cacao roots because i need microbial root DNA for metagenomic studies, I want to know if there is any suggestion...
07 July 2018 9,811 1 View
I'm currently preparing DNA from six populations of fish for whole genome sequencing on an Illumina HiSeq machine. Unfortunately half my populations have a low A260/A230 score (~1.6) indicating...
06 June 2018 5,186 4 View