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Questions related to DNA Extraction
I used boiling method to extract DNA from lactic acid bacteria. as follow procedure: Fifty microliter of dH2O is added to the pellet. After vortexing, the sample is boiled at 98°C for 10 minutes...
06 June 2019 2,058 4 View
I recently did a whole genome sequence on Plasmodium falciparum (Illumina , NGS). I have the data but i have been stuck at obtaining the sequences for specific genes of interest. Any tools or...
06 June 2019 8,996 3 View
Dear All, Can we use Tween 20 instead of triton X in 0.1% PBT-T(I assume T stands for triton X only) for immunofluorescence ? Also, can tween 20 be replaced by tryton in IP wash buffer?
29 May 2019 6,448 3 View
Instead of using an OTU table for PiCrust, can I ask PiCRUST to give me the function of a particular genera eg Bacteroides?
23 April 2019 864 7 View
I want to make a masterbatch for extrusion (I will use a twin-screw extruder) I searched the method to make masterbatch, and one example is mixing powder and cnt (melt-blending, or centrifugal...
21 April 2019 3,633 3 View
Brain tissue that has been fixed during perfusion with 4% PFA and stored for several months in 30% sucrose, 1X DBPS solvent for both solutions. Wondering whether it's possible to extract DNA from...
04 April 2019 774 4 View
Hi all, I am extracting DNA from blood, and due to waiting for buffer solutions to arrive, the blood samples appear to have dried in their plates. Some were clotted, so have had EDTA added to...
19 March 2019 5,163 3 View
Hi, I would like to perform simultaneous RNA and DNA extraction from rat brain tissue. Thinking about using the Qiagen AllPrep kit, but I have two questions: - the dissection of the brain tissue...
03 March 2019 9,941 4 View
I'm trying to find the absolute abundance of bacterial species in a bovine fecal sample (fecal microbiome). Can anyone suggest the best method to achieve this?
01 March 2019 6,972 6 View
Does anyone have recommendations of tools/techniques for getting the filter out of a 0.2 micron Minisart Cellulose Acetate filter unit. I've filtered water samples and want to extract DNA from the...
22 February 2019 7,164 2 View
hello, what is the best method for extracting bacterial dna from honey? thank youu
20 February 2019 415 6 View
After some consulting I decided to use a commercial pcr cleanup spin column kit to concentrate DNA from my CHIP for sequencing. The DNA was in elutate of sodium bicarbonate and 1% SDS....
02 February 2019 7,558 2 View
02 February 2019 665 7 View
I am doing DNA extraction using CTAB method from different fungal strains including monilinia spp. isolated from peach. My DNA concentration is very low. Is there any suggestion to improve the...
22 January 2019 8,900 3 View
According to the following figure, we can know how lactic acid bacteria can make clear area. But I'm so confused.. because other bacteria also can make other acid besides lactic acid.. And I think...
07 January 2019 6,335 8 View
Can the moleculer weight of PEG (8000 and 6000) cause a noticeable effect in phage precipitation???
23 December 2018 785 3 View
I have been trying to validate a qPCR reaction, where the expected amplicon is 98 bp long. Initially, I made standard dilutions from qualitative PCR product. However, when I ran it the first few...
20 December 2018 4,472 9 View
12 December 2018 9,960 4 View
Our ultimate goal is to measure microRNA gene expression. We have tried several kits including Qiagen and all gave poor values for RNA concentration and 260/280. Any suggestions or ideas would be...
12 December 2018 3,776 6 View
Dear all, Are there any works that describe the positive relation between the diel vertical migration speed and the cells survivability for phytoplankton cells? Thanks you in advance Best...
08 December 2018 3,362 2 View
Dear Colleagues, I am looking for an adhesive film or filter that will decay when submersed in lysis buffers such as ATL (Qiagen). I once had a sample of such filters a couple of years ago, but I...
30 November 2018 4,290 2 View
So we use Ethidium bromide to stain our agarose gels for dna extraction and analysis. The software in our gel doc is malfunctioning so we are unable to capture images. So now we are using our...
27 November 2018 3,045 7 View
I am currently working on dna extraction from soybean leaves. Months prior, I could get clean and clear bands of DNA extracts when viewed in the agarose gel. But this time around, when I checked...
14 November 2018 4,148 8 View
11 November 2018 6,190 2 View