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Questions related to DNA Extraction
I want to extract RNA from fish blood for making cDNA. What would be suitable temperature for preserving the blood sample in case of delayed extraction of RNA from the blood? Does RNA degrade if I...
07 July 2017 9,058 5 View
Can any one provide the standardized protocol for Gnomic DNA isolation from aerobic bacteria. I am doing with Kit method and its too cost so i want to isolate DNA with manual methods
07 July 2017 228 4 View
I need to replicate the results obtained with this lysis buffer, but manufacturing it myself would allow me to lower costs. I have read the SDS, I know it has guanidinium thiocyanate and EDTA, but...
07 July 2017 3,438 0 View
We would like to extract DNA from whole blood using the Prepman Ultra Sample Reagent but we cannot find a protocol to do it. Could you help us?Thank you!
07 July 2017 4,206 22 View
I want to avoid ethanol as it is known to hinder downstream amplification process. Also, I am in a process of developing an automated diagnosis of ZIKA and would want to avoid...
07 July 2017 5,382 3 View
I'm not sure if research gate allows a this type of questions but it's worth trying. Dear community can someone help me with an online version of the following research article: Doyle J. J,...
07 July 2017 8,360 3 View
My student has bundles of bee-collected pollen from a phenology study, and would like to get DNA barcode data from them. We have tried following protocols involving liquid N2, and grinding 0.1g of...
07 July 2017 1,073 3 View
I want to use the SpeedVac concentrator to concentrate some gDNA samples i have extracted from a co-culture experiment. The samples contain both prokaryotic and eukaryotic DNA. Eventually only the...
07 July 2017 6,119 2 View
I usually use distilled water for primer dilution, i never got problem but one of GC rich (63%) primer getting degraded often. which one can be better for GC rich primer dilution TE or water if TE...
07 July 2017 2,980 26 View
Currently I am trying to troubleshoot difficult extractions. These extractions came from a wing punch from a bat. I am struggling to amplify the DNA through PCR. I have reduced the amount of...
07 July 2017 284 3 View
I am working with Sera-Mag paramagentic beads to isolate whole genomic DNA from human fibroblasts. I follow the general protocol: bind DNA to beads with PEG/NaCl solution, place on magnetic rack,...
07 July 2017 1,835 9 View
I need to purify some genomic DNA from a human skin punch biopsy. In the past, I have done this with the Qiagen DNEasy Blood and Tissue kit. This yields good quality DNA for routine PCRs,...
07 July 2017 8,547 3 View
I am looking for recommendations on a kit to extract RNA from whole blood from a songbird species. We cannot take a lot of blood from the birds since they are small. Are there kits that allow for...
07 July 2017 3,132 5 View
I'm macrodissecting very small tumours (max 1 cm) using a scalpel and I'm using non-silanized glass slides.To extract the DNA I use a comercial kit with silica colums. My question is: the...
07 July 2017 5,950 0 View
Is there any specific kit available for the extraction?
07 July 2017 7,668 5 View
I try already 2 protocols but there are not good enough, the nanodropo and the electrophoresis gel does not give us good results!
07 July 2017 9,832 3 View
How can extract DNA genomic with high purity in fungal with the CTAB method?
07 July 2017 6,994 6 View
I am looking for a protocol for DNA extraction from bucal swabs or saliva but most of them use kits and I only have reactives for traditional methods.
07 July 2017 7,080 5 View
In order to get a higher concentration of DNA with Molysis extraction kits (Molzym-Coimplete5 and UMD) we are eluting columns with 50 ul, instead of 100 ul as recommended. Can it affect DNA quality?
07 July 2017 1,678 1 View
I see in the Qiagen handbook that it recommends you buy their proprietary centrifuge in order to get high enough rpms (3000 & 6000) necessary for optimal DNA extractions. I have access to a...
07 July 2017 1,698 2 View
I 'm using the Qiagen DNAeasy Power Water kit which is supposed to be the former MoBio Power water DNA extraction kit. Does anyone has experience with it? I'm getting some issue in having...
07 July 2017 4,877 5 View
Please provide me practically viable solution.
03 July 2017 2,213 6 View
03 July 2017 4,738 4 View
This DNA extraction is from a filter that has had lake water pass through it with the goal to capture microbial DNA on a .22 um filter.PEG 6000 is normally used for this protocol but PEG 8000 was...
30 June 2017 633 2 View