Hello,
I am struggling with library preparation for Illumina sequencing. Currently I prepared 6 libraries, but only 2 of them were OK and ready for sequencing. The other 4 contained high amount of adapter dimers (from 20 to 70%). I am working with human stool samples. For normalization I used SequalPrep™ Normalization Plate Kit (cat. no. A1051001). For ligation I used KAPA HyperPrep Kit (code KK8503) and for qPCR KAPA Library Quantification (code KK4824). 4 of the 6 libraries represent almost full plate = 90 samples, two of them are only half in samples. Do you think it is neccesary to adjust the amount of adapter for smaller libraries? I am using 5 microliters for each library. Or is the ligation problem (I used expired kit)?
Thank you so much in advance.
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