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Questions related to DNA Extraction
Hi all, I'm trying to clean up some DNA for genomic sequencing (Nanopore and Illumina), but i'm still struggling with the purity of my DNA. My 260/280 ration is ok, that is not an issue, however...
03 March 2018 9,294 3 View
I have extracted DNA oligonucleotide of size 77 bp from a crushed 10% PAGE gel using 3 rounds of LiClO4 in water. The extracted elute was dried down in a vacuum centrifuge to a certain volume and...
03 March 2018 6,791 5 View
Can anyone identify method to inoculation soil in algae or cyanobacteria to determine the affect biological soil crust on erosion by jet erosion test i want use this mold to grow soil crust on...
25 February 2018 595 2 View
1. 1.5ml of the bacterial culture was poured into the microcentrifuge tube and then centrifuged at five minutes at 10,000 rpm. 2. the supernatant was discarded and the pellet was left at the...
01 January 2018 3,382 3 View
During search in GenBank database I found in some sequences the following comment: GenBank staff is unable to verify sequence and/or annotation provided by the submitter. so, what are the reasons...
29 December 2017 3,210 6 View
Dear all, I performed phage DNA extraction by phenol/ chloroform followed the below steps: - Precipitation of phage particle using PEG8000 with the presence of NaCl - Add DNase, RNase to digest...
11 November 2017 3,013 3 View
Fresh plant tissue is recommended for DNA extraction while plant tissue frozen at -20 degree centigrade can produce poorer DNA output. I am interested to know how big the difference is when DNA...
11 November 2017 7,754 5 View
we are extracting protein from fruits. in nearly all buffers, CHAPS is used but i don't have this detergent.
31 October 2017 3,013 0 View
DNA extraction protocol using Chelex-100 is cheap and very useful technique. However, the protocol varies between lab to lab, or people to people. My lab is fungal taxonomy lab, so DNA is...
19 October 2017 8,234 1 View
Hello everyone, I am looking for some advice on the CTAB method for DNA extraction from faecal samples (from bat droppings). I am following the below written method but I am a bit unclear on the...
18 October 2017 5,534 1 View
I want to extract cp DNA from some desert plant, i have issue in extraction of cp DNA i am following An Improved Chloroplast DNA Extraction Procedure . After second centrifugation 3500G i get...
10 October 2017 9,210 3 View
10 October 2017 5,413 1 View
10 October 2017 6,612 1 View
I want to perform clean up on my digests but currently out of ERC from minElute clean up kit. Can I substitute it with buffer QG ? These are all from qiagen. Somebody told me I could do this but...
05 October 2017 8,273 3 View
Hi, I'm working on metagenomic analysis of undefined mixed culture of algae-bacteria culture using 16s/18s rRNA analysis using Illumina sequencer. Any suggestion for the best kit to do total DNA...
16 August 2017 726 2 View
08 August 2017 3,903 2 View
i recently ran a DNA extraction and isolation experiment on an an indigenous plant but no bands were observed on the agarose gel. What could be the reason for not obtaining DNA even though...
08 August 2017 7,996 0 View
Hi everyone, I am currently running a multiplex PCR, and product size varies from 120 bp to 500 bp. I have checked all those temperature on thermo machine, which are fine according to the paper I...
26 July 2017 9,742 4 View
I want to use the SpeedVac concentrator to concentrate some gDNA samples i have extracted from a co-culture experiment. The samples contain both prokaryotic and eukaryotic DNA. Eventually only the...
25 July 2017 6,710 2 View
Hi, I have a primer which produces multiple amplicons, so i observe multiple bands on gel. I was expecting that, and i would like to cut the bands, extract DNA, and use it as template for another...
19 July 2017 2,088 38 View
How can extract DNA genomic with high purity in fungal with the CTAB method?
17 July 2017 8,593 6 View
I am looking for a protocol for DNA extraction from bucal swabs or saliva but most of them use kits and I only have reactives for traditional methods.
16 July 2017 6,569 5 View
As I said here: https://www.researchgate.net/post/Looking_for_a_DNA_extraction_protocol_from_adult_beetles_Coleoptera I finally extracted the DNA from adult beetles using a CTAB protocol. But the...
07 July 2017 1,524 8 View
Please provide me practically viable solution.
07 July 2017 6,427 6 View