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Questions related to DNA Extraction
I used HotShot protocol to extract DNA from pig ears, needing to do PCR RFLP. Most of my samples I had successful PCR first time, and succesfsul RFLP. For some samples I only get very faint bands,...
27 August 2019 9,240 6 View
Dear all, I have used ATMAB protocol for pines DNA extraction. But, after incubation in a heat bath, the solution started black in colour. I applied washed with chloroform for carotenoids but not...
26 August 2019 5,673 3 View
I am researching two isoforms of an endolysosomal ion channel. I have found that when one is knocked down with siRNA, the expression of the other increases and vice versa. I cannot see this being...
21 August 2019 6,928 2 View
Hi there, I have been performing fmoc removal on my oligonucleotides using 10%piperidine. However, it seems that piperidine was found still left in solution after ethanol precipitation. Dos...
08 August 2019 9,771 3 View
08 August 2019 5,018 3 View
Iwill be so grateful if any researecher can share me protocol of DNA extraxtion using SDS/PK/CTAB method . thanks
08 August 2019 8,706 2 View
To conduct proteomics of lysosomes, I am trying to replicate the "Lyso-IP" protocol described in the paper ; G.A. Wyantet al., Science10.1126/science.aar2663 (2018). Unfortunately, I can not...
06 August 2019 6,252 4 View
I'm currently working on the proposal for my first project. the plan is to take the samples with swabs, place them in TSB then use the bacteria from the broth in a cell culture. how do i quantify...
04 August 2019 2,099 6 View
I used Mag-bind total pure NGS beads in 1.2x concentration to clean up my cDNA libraries that are to be used for sequencing. I found that I lost 80% of library during cleanup, is that expected or...
18 July 2019 9,954 1 View
Hello everyone, I have filtered some samples of lake water through PES filters (pore size 0.22 um, filter diameter 73 mm). From these filters I will need to extract DNA for metagenomic...
07 July 2019 306 3 View
Using bleach (10-20%) for cleaning metal instrument is corrosive. Allowing 5 minutes contact time for bleach followed by wiping with alcohol or water reduces the prescribed contact time for...
06 June 2019 3,435 4 View
I used boiling method to extract DNA from lactic acid bacteria. as follow procedure: Fifty microliter of dH2O is added to the pellet. After vortexing, the sample is boiled at 98°C for 10 minutes...
06 June 2019 1,908 4 View
I recently did a whole genome sequence on Plasmodium falciparum (Illumina , NGS). I have the data but i have been stuck at obtaining the sequences for specific genes of interest. Any tools or...
06 June 2019 8,841 3 View
Dear All, Can we use Tween 20 instead of triton X in 0.1% PBT-T(I assume T stands for triton X only) for immunofluorescence ? Also, can tween 20 be replaced by tryton in IP wash buffer?
29 May 2019 6,292 3 View
Instead of using an OTU table for PiCrust, can I ask PiCRUST to give me the function of a particular genera eg Bacteroides?
23 April 2019 790 7 View
I want to make a masterbatch for extrusion (I will use a twin-screw extruder) I searched the method to make masterbatch, and one example is mixing powder and cnt (melt-blending, or centrifugal...
21 April 2019 3,546 3 View
Brain tissue that has been fixed during perfusion with 4% PFA and stored for several months in 30% sucrose, 1X DBPS solvent for both solutions. Wondering whether it's possible to extract DNA from...
04 April 2019 652 4 View
Hi all, I am extracting DNA from blood, and due to waiting for buffer solutions to arrive, the blood samples appear to have dried in their plates. Some were clotted, so have had EDTA added to...
19 March 2019 4,970 3 View
Hi, I would like to perform simultaneous RNA and DNA extraction from rat brain tissue. Thinking about using the Qiagen AllPrep kit, but I have two questions: - the dissection of the brain tissue...
03 March 2019 9,840 4 View
Does anyone have recommendations of tools/techniques for getting the filter out of a 0.2 micron Minisart Cellulose Acetate filter unit. I've filtered water samples and want to extract DNA from the...
22 February 2019 7,085 2 View
hello, what is the best method for extracting bacterial dna from honey? thank youu
20 February 2019 252 6 View
After some consulting I decided to use a commercial pcr cleanup spin column kit to concentrate DNA from my CHIP for sequencing. The DNA was in elutate of sodium bicarbonate and 1% SDS....
02 February 2019 7,421 2 View
02 February 2019 533 7 View
I am doing DNA extraction using CTAB method from different fungal strains including monilinia spp. isolated from peach. My DNA concentration is very low. Is there any suggestion to improve the...
22 January 2019 8,780 3 View