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Questions related to DNA Extraction
Are there any significant issues with using standard plastic falcon tubes for DNA extraction with chloroform? How long can chloroform be in contact with polypropylene for it to have a negative...
18 October 2019 3,981 4 View
Please suggest me suitable kits for rolling circle amplification (RCA) of begomoviruses other than RepliG (Qiagen, Germany).
13 October 2019 1,481 1 View
Tried various protocols none is working. Generally getting DNA contaminated with phenols and proteins.
10 October 2019 7,971 5 View
10 October 2019 3,552 4 View
10 October 2019 8,872 4 View
I'm interested in fish genetics and phylogenogenetics. I might choose this topic for my PhD, however I have a concern of how samples are acquired for these kinds of studies. For fishes >8-10...
10 October 2019 602 3 View
I looking for a method to measure biomass of terricolous lichen species and organic acids of these crusts at soil
02 October 2019 9,217 1 View
Does anyone have a protocol or experience with extracting genomic DNA from very small sample volumes of 10-30 microliters? The sample is a microbial enrichment from a sewage treatment plant.
01 October 2019 7,121 1 View
Briefly, I need a cost and time effective method - which completely exludes the possibility of contamination between samples - in order to get ranaviral DNA from the liver of EtOH stored...
18 September 2019 8,526 6 View
This is my first time using commercialised PCI (25:24:1) solution. After adding equal amount of PCI into my supernatant, two of my samples did not form two layers as they should be. After...
09 September 2019 5,052 3 View
09 September 2019 5,689 7 View
04 September 2019 9,725 3 View
Iwill be so grateful if any researecher can share me protocol of DNA extraxtion using SDS/PK/CTAB method . thanks
30 August 2019 2,279 3 View
I used HotShot protocol to extract DNA from pig ears, needing to do PCR RFLP. Most of my samples I had successful PCR first time, and succesfsul RFLP. For some samples I only get very faint bands,...
27 August 2019 9,338 6 View
Dear all, I have used ATMAB protocol for pines DNA extraction. But, after incubation in a heat bath, the solution started black in colour. I applied washed with chloroform for carotenoids but not...
26 August 2019 5,780 3 View
I am researching two isoforms of an endolysosomal ion channel. I have found that when one is knocked down with siRNA, the expression of the other increases and vice versa. I cannot see this being...
21 August 2019 7,031 2 View
Hi there, I have been performing fmoc removal on my oligonucleotides using 10%piperidine. However, it seems that piperidine was found still left in solution after ethanol precipitation. Dos...
08 August 2019 9,843 3 View
08 August 2019 5,134 3 View
08 August 2019 8,803 2 View
To conduct proteomics of lysosomes, I am trying to replicate the "Lyso-IP" protocol described in the paper ; G.A. Wyantet al., Science10.1126/science.aar2663 (2018). Unfortunately, I can not...
06 August 2019 6,373 4 View
I'm currently working on the proposal for my first project. the plan is to take the samples with swabs, place them in TSB then use the bacteria from the broth in a cell culture. how do i quantify...
04 August 2019 2,192 6 View
I used Mag-bind total pure NGS beads in 1.2x concentration to clean up my cDNA libraries that are to be used for sequencing. I found that I lost 80% of library during cleanup, is that expected or...
18 July 2019 10,067 1 View
Hello everyone, I have filtered some samples of lake water through PES filters (pore size 0.22 um, filter diameter 73 mm). From these filters I will need to extract DNA for metagenomic...
07 July 2019 410 3 View
Using bleach (10-20%) for cleaning metal instrument is corrosive. Allowing 5 minutes contact time for bleach followed by wiping with alcohol or water reduces the prescribed contact time for...
06 June 2019 3,537 4 View