How to differentiate the economic productivity in agriculture and in manufacturing sector?

Most of the references I found were on the economic productivity in agriculture but very limited application in manufacturing sector. I would appreciate if anyone could share the references on...

15 February 2021 8,342 7 View

How to solve error "Cannot find position restraint file restraint.gro (option -r)." on GROMACS?

Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...

01 February 2021 780 3 View

How do we decide BSA percentage in our buffer solution?

Hi all, I am trying to test my polymer stability in biological medium. I know how to make 1% /5% etc of BSA in buffer but I am not sure how do we actually decide this percentage volume. Is there...

05 January 2021 649 2 View

How to solve PSIM Thermal Module - C Block Error (MOSFET losses analysis)?

08 December 2020 3,827 5 View

Prevent contamination of solution in oven?

Hi all, I am trying to test stability of my electrode immersed in solution. I intent to keep them in oven for 3 weeks as to represent accelerate ageing. However, my worry is that the solution...

28 October 2020 8,351 2 View

Photometric method for TSS testing?

is there any article journal or reliable writing that explain about testing the total suspended solid (TSS) in water using photometric method as most of the article that i found using gravimetric...

16 June 2020 7,036 1 View

How to convert odd ratios to log odds?

Hi, How can I convert odd ratios to log odds and present them in a graph? Thank you

09 February 2020 6,215 3 View

Preservation of freshwater samples (in sterivex filter) using dry ice+ 100% ethanol bath for subsequent metatranscriptomics analysis?

17 December 2019 9,172 2 View

Do you think that six sigma method are important in the hypermarket system? why?

Tunas Manja Group operates the retail store as TMG mart and facing problem in the sorting process of the items. As we know, six sigma is a set of management techniques intended to improve...

04 November 2019 5,155 3 View

Do you have experiences in introducing the biotechnology concept or basic concept of DNA to high school students?

Hello everyone, Would anyone mind sharing your experience in teaching about principle of biotechnology and concept of DNA to high school students? I am planning to do a community service in that...

01 September 2019 2,334 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

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17 February 2021 4,217 3 View