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Questions related to DNA Extraction
I'm going to measure chlorophyll spectrophotometrically. Does anyone have a tried-and-tested, detailed protocol?
15 July 2023 4,961 4 View
I registered for an online course (Bioinformatic Methods 1) from Coursera, in one of the labs we are dealing with the MG-RAST online server. Im searching for the "sequence Break down / predicted...
11 July 2023 8,899 1 View
I prepared the blood agar plate and stored it for two weeks and then taken out and did the streaking of different unknown bacteria onto the plate. After 24 hours, the entire Petri plate change...
06 July 2023 6,169 2 View
I'm having a question regarding the metagenomic analysis online server, I'm using it in one of the courses, and we are comparing two sequences, However, I start the analysis I got the following...
04 July 2023 605 0 View
The growth of SF21 cells we are currently cultivating in SFM medium is not ideal. Is there any way to improve it?
01 July 2023 6,510 1 View
Hi all, here in Chile we are facing a brutal shortage of consumables of Merck-Sigma-Millipore, specially after the fusion of Merck with Sigma and the pandemia, anyway the thing is that ultra...
29 June 2023 3,188 1 View
Note that while bootstrapping has an element of randomness to it, there is fairly large difference between those nodes that are well-supported and the others. What it means that the bootstrap has...
22 June 2023 6,661 4 View
I am amplifying a PCR fragment from virus. after I ran the sample in agarose gel, the marker looks just fine but the samples ran curved. can anyone explain. Thanks
21 June 2023 3,236 2 View
Hi every one, I our Skin Lab research, we focus in Pemphigus Vulgaris diseases, specific the association of ST18 gene with PV. We read the article "Etesami, Ifa, et al. "The association...
06 June 2023 1,331 2 View
Hi, I am looking for a protocol for HBV RNA quantification. Could you please suggest one? I found a few papers, but I am still facing some issues.
06 June 2023 3,860 0 View
Along with reference please
29 May 2023 7,225 2 View
I want to know the stationary phase PGPR pseudomonas such as fluorescence pseudomonas. can anyone help me what is the OD of those bacteria in stationary phase?
05 May 2023 9,622 3 View
This is a questionnaire for academic research in the hotel industry. I really appreciate your time if you fill out this form: https://forms.gle/fqFuHMk3a161FHNJ7
30 April 2023 5,061 0 View
Hi, deal all. We are trying to detect a secreted protein, about 20-35kDa, in the cell culture. What we used was the Amicom ultra-10kDa. We rinsed the filter with ddH20, centrifuged cuture...
28 April 2023 6,386 6 View
What is the difference between cellular and non cellular plastic? please give me a method to measure the cellular and non cellular plastic? please give me a method
18 April 2023 3,687 1 View
Hello, I used to work with MEGA 11 to build the phylogenetic trees etc. And to this end I got Huawei MateBook D 16 16" i7-12700H - 16GB RAM - 512GB with Win11. And I have a problems with runnig...
31 March 2023 8,085 1 View
Both have same Refractive index.
14 March 2023 5,789 1 View
Hi In the measurement of k232 of olive oil what are the solvents used in this procedure? Are they always have to be (cyclohexane or iso-octane) and why? Thanks in advance
10 March 2023 3,333 0 View
The holothurian (sea cucumber) genus Thyone appears to be a "supergenus" with about 66 species worldwide and is in need of urgent revision. Apparently the taxonomy and phylogeny of this group is...
02 March 2023 592 2 View
Was just wondering what the recommended amount of time for cells to stay in HBSS is. Also what happens to the cells if they stay in this solution for prolonged amounts of time.
01 March 2023 8,092 1 View
I need to confirm that the antibacterial activity of the lactic acid bacterial strains is by bacteriocins(the antibacterial peptides). Are there any biochemical tests to confirm it?
15 February 2023 4,922 3 View
what are the factors that can contribute to the difference in efficacy between DNA and RNA encapsulation using microfluidic techniques?
09 February 2023 2,235 0 View
Relation between high yield plants fertilizer
05 February 2023 572 3 View
Either for a single gene or a list of genes
03 February 2023 3,262 0 View