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Questions related to DNA Extraction
What type of adsorption mechanism is used for organic compound removal and biochar for removal of organic and inorganic pollutants from industrial wastewater?
24 December 2022 2,603 2 View
Hi guys, I am working on Akk culture and find that Akk cannot be well mixed even after vortexing and pipetting. Moreover, what counting method do you suggest? Akk cannot be well observed using...
16 November 2022 4,809 0 View
Hi Community, Recently I have submitted samples for amplicon and found an unusually high number of reads being present in my negative control blank samples. Yet, when I ordinated those samples in...
20 May 2022 2,257 0 View
Hello, I have some SAED patterns taken from ZrN/Cu coatings deposited by reactive magnetron sputtering. When there is a preferred orientation in the microstructure, the corresponding ring is not...
03 May 2022 258 3 View
Hello, I need your help. I am working on a SARS-Cov2 seroprevalence study. I will isolate the plasmas using a ficoll gradient; I would also like to keep the PBMCs for future studies. I want to...
11 February 2022 4,259 3 View
I plan to use BALB/c mice for studying pneumonia caused by the mouse-adapted influenza virus (A/PR8/H1N1). I want to know, what is the requirement to determine the sex of an animal? It seems...
24 November 2021 3,352 4 View
Hello, I diluted stock solution of both MTX and doxo in RPMI today (separately) and was hoping to use the rest of the dilution tomorrow for some Jurkat cells (less than 24 hours after dilution),...
11 October 2021 6,069 2 View
Hi everyone, My cols and I got the QIAamp Fast DNA Stool Mini Kit in order to get DNA from sea lion scats to analyze the diet of these animals. However, a colleague warned not using this "Fast"...
17 June 2021 6,301 0 View
Hello, I've been having an issue with my PCR amplicon appearing smaller on a gel than it should be. For reference, the band should be ~500bp, but it consistently runs closer to 250 bp according to...
13 June 2021 8,584 6 View
I've been measuring the gas emission of a lab-scale peatland experiment with a electrochemical sensor. The output of the sensor measures the concentration in ppm. I want to change this data to EF....
26 May 2021 6,958 1 View
If it is possible, suggest me a protocol, or suggest me a researcher or lab where this kind of research is active. Particularly, the main interest is cell division rate and cell morphology change...
10 May 2021 5,325 5 View
I am aiming to extract total DNA (eDNA) from samples containing cells (plant and animal), organelles (mitochondria and chloroplasts) and dissolved DNA. Using mechanical lysis (bead-beating) could...
05 May 2021 5,061 0 View
Hi everybody, can anybody suggest how to extract DNA from insect samples conserved in silica gel (in my case, mosquitoes)? Does anybody know a commericial kit which does that or a particular...
11 February 2021 3,642 4 View
- The Eppendorf tube was then placed in a microcentrifuge. Notice how it is balanced with another tube placed opposite it in the centrifuge drum to ensure smooth rotation. Other information...
25 January 2021 2,717 3 View
I would like to run molecular diversity indices using Tamura & Nei as a distance method for calculating nucleotide diversity of mtDNA control region sequence. It is difficult for me to...
20 January 2021 5,311 1 View
Hi there, I'm looking for a High Molecular Plant DNA Extraction Kit for subsequent Nanopore sequencing. Does anyone have experience using commercially available kits? Any...
04 January 2021 9,587 4 View
I want to separate DNA from human old clotted blood . I haven't any idea how to extract DNA from it. Your kind expertise and thought would be appreciated.
08 October 2020 8,016 2 View
Is it an effective method to isolate DNA from Gram-negative bacteria with only the use of a boil and freeze-thaw protocol, without any chemical purification? Also, is there a media to maintain the...
07 October 2020 7,059 8 View
I spiked in 4-8 log CFU/ml of Lactococcus lactis into milk, and use DNeasy Powered Food Kit to extract DNA from it. Nanodrop 2000 was used to determine the values. The yield and quality are weird:...
05 October 2020 6,317 4 View
We shall collect biopsies from skin/hoof of animals for bacteriological microbiome analysis. We need suggestions on DNA extraction kit that may be used for this. We prefer to keep the biopsies in...
25 September 2020 6,974 4 View
I will be extracting trypanosome DNA from mid-guts of tsetse flies for LAMP analysis. Kindly advice on protocol to adopt for maximum yield and benefits of Chelex method over the regular method....
24 September 2020 648 4 View
Is there any way in which we can check whether the DNA inside the cell is negatively or positively supercoiled?
13 September 2020 8,969 2 View
Does anyone have experience of extracting DNA out of dairy cow's milk? I am interested in the DNA present in the B-cells. I have done extraction from blood but want to try extraction from milk. I...
03 September 2020 3,636 5 View
I have a large set of samples to extract DNA from, and am trying to plan out the most efficient way to process them. I have a few options, but the most efficienct would be to first process all of...
17 August 2020 2,942 9 View