DNA Extraction from old clot blood?

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How can Gamma Ray API units be converted to CPS (counts per second)?

Oil and gas companies run gamma ray tool and take readings in API units while Gamma ray is also noted in cps, so how these units can be converted into each other?

24 February 2021 7,545 2 View

Is it possible that non-sea salt fraction has negative value?

Hi, I'm trying to calculate sea salt and non sea salt fraction from water soluble ion data (anion and cation). I calculate sea salt fraction by using using ssNa+ as specific marker and knowing the...

29 December 2020 9,813 9 View

How to do verification of the reaction steps of PCR conditions?

One of the reviewers in my submitted paper ask me for the "verification of the reaction steps of PCR conditions". how to argument to this comment, do i have to add reference or i have to verify...

09 September 2020 9,325 9 View

How do I edit a request?

I sent a request for an article to a professor at UPenn. This is my dream school and I was doing research on their Criminology department. But the request sent before I finished typing and so it...

13 August 2020 9,810 3 View

Can anyone share with me their protocol for Picric acid for Pico Sirius Red?

1) How do I dissolve the Sirius Direct 80 Red? I know it should be in saturated Picric acid solution. But how many grams of picric acid should I add to dissolve in water? What should the...

22 March 2020 233 3 View

Can Cochrane Risk of Bias tool be used to critique pilot/feasibility RCTs?

I have a couple studies that were classed as pilot RCTs that I'm not sure if they are suitable to be critiqued using the Risk of Bias tool (Cochrane) for a systematic review. My concern is the...

30 December 2019 2,550 3 View

What is the latest trend or updates in the study of Gamification in education, especially in enhancing engagement and motivation?

Seeking the latest development in gamification research particularly in education whereby gamification is used to increase student engagement with the lessons and later improve their motivation to...

07 October 2019 2,743 2 View

How to check secretory protein concentration from cell culture media by spectrophotometer?

I am going to culture a cell line with 20% FBS in IMDM medium. Those cell will secrete protein. I need to check the protein concentration from the culture medium by using spectrophotometer. Can...

17 September 2019 6,047 1 View

How to test the statistical significance of the difference between a mean and a median?

For two quantitative data sets (One is normally distributed and summarized as means, while the other is not normally distributed and summarized as medians), how to test the statistical...

04 May 2019 6,391 18 View

How to determine quantitatively Cu and Zn in Brass alloy?

Dear all I am searching for a suitable method for the determination Cn and Zn in Brass alloy. I need a method for school grade level students, with out the use of instruments (AAS, Photometer...

04 April 2019 7,707 8 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View