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Questions related to DNA Extraction
I’m working on a DNA extraction optimization for Ascaris, but we have no fecal samples that are positive for Ascaris. We have obtained some Ascaris eggs, and I will be spiking negative human stool...
12 December 2019 3,289 7 View
I am currently facing difficulty in the DNA extraction because of the viscosity of the sputum samples. I am using the Chelex based extraction method which does yield in DNA but it is inefficient...
12 December 2019 3,266 1 View
Dear experts, I observed the phenomenon that when we leave the blood agar plate for over 3 months, there was a change from red to extreme dark red ( or light black color). I would like to ask...
10 December 2019 6,562 4 View
I am developing a phylogeography workflow and I need PERMUT cpSSR to compute NST and GST for mitochondrial Cytochrome c Oxidase (COI) haplotypes so that I can test for genetic differentiation...
09 December 2019 701 2 View
The blood I am using has been collected from Nile Crocodiles and is stored at -20°C.
21 November 2019 1,130 3 View
I extract DNA from various sludge samples according to the method by Griffiths et.al (2000). By now I did not have any problem, even in case of sludges which contain a great amount of silage and...
15 November 2019 2,603 3 View
Hello, We recently obtained some insect samples (Asphondylia) collected a few months ago by collaborators. The individuals were freeze dried, placed into pure ethanol, and shipped to us on dry...
11 November 2019 1,199 2 View
11 November 2019 8,325 3 View
11 November 2019 1,544 3 View
I'm looking into extracting DNA from buccal cells. I have no experience in using this source material and was wondering if there was a general consensus on preferred method? Yield would be a...
11 November 2019 9,230 3 View
I have some sporocarps of gilled mushrooms frozen at -20°C and I want to regenerate the mycelia from them. can it be used as inoculum? if YES, Please suggest me available methods to do so.
11 November 2019 6,178 5 View
Are there any significant issues with using standard plastic falcon tubes for DNA extraction with chloroform? How long can chloroform be in contact with polypropylene for it to have a negative...
18 October 2019 3,866 4 View
Please suggest me suitable kits for rolling circle amplification (RCA) of begomoviruses other than RepliG (Qiagen, Germany).
13 October 2019 1,398 1 View
Tried various protocols none is working. Generally getting DNA contaminated with phenols and proteins.
10 October 2019 7,875 5 View
10 October 2019 3,453 4 View
10 October 2019 8,718 4 View
I'm interested in fish genetics and phylogenogenetics. I might choose this topic for my PhD, however I have a concern of how samples are acquired for these kinds of studies. For fishes >8-10...
10 October 2019 451 3 View
I looking for a method to measure biomass of terricolous lichen species and organic acids of these crusts at soil
02 October 2019 9,148 1 View
Does anyone have a protocol or experience with extracting genomic DNA from very small sample volumes of 10-30 microliters? The sample is a microbial enrichment from a sewage treatment plant.
01 October 2019 7,052 1 View
Briefly, I need a cost and time effective method - which completely exludes the possibility of contamination between samples - in order to get ranaviral DNA from the liver of EtOH stored...
18 September 2019 8,468 6 View
This is my first time using commercialised PCI (25:24:1) solution. After adding equal amount of PCI into my supernatant, two of my samples did not form two layers as they should be. After...
09 September 2019 4,968 3 View
09 September 2019 5,613 7 View
04 September 2019 9,652 3 View
Iwill be so grateful if any researecher can share me protocol of DNA extraxtion using SDS/PK/CTAB method . thanks
30 August 2019 2,176 3 View