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Questions related to DNA Extraction
I'm doing my research on moss and right now i'm doing dna extraction from moss sample. I use the Qiagen Dneasy plant mini kit but i didn't get any result. I don't know what i do wrong. I thought...
02 February 2020 1,625 2 View
I performed DNA extraction using the Quiagen Blood & Tissue Kit on a set of liver samples; and happened to find tissue in the final DNA extract (black precipitate at the bottom). I did add the...
02 February 2020 3,697 3 View
Dear all, I performed DNA extraction using Qiagen DNeasy Blood and Tissue Kit, followed the protocol with an addition of 2ul RNase A with incubation of 2 min at R.T after adding proteinase K....
02 February 2020 5,548 4 View
Recently I performed a DNA extraction using Qiagen DNeasy kit and the results show a 260/230 ratio of 1.05 likely due the presence of phenol or other organic contaminant... I have read that you...
02 February 2020 7,231 3 View
Hello everyone, I would like you to please help me in how to generate the inputs for use in the BAPS. I have sequences from the IOC gene. Thank you!
11 January 2020 758 1 View
I am attempting to set up a QC laboratory to test viral samples isolated from insects. The test will include gel electrophoresis and qPCR analysis. What I want to know is what type of water should...
17 December 2019 941 5 View
I work the research about stabilization of soil by Biological soil crust especially algae (Cyanobacteria and green algae) Could anyone please help me about this topic soil stabilization using...
17 December 2019 808 2 View
I am doing a project for the evaluation of MinION-sequencing pipelines regarding MRSA and non-MRSA in terms of identification and resistance gene detection from cultured clinical isolates. My...
12 December 2019 8,179 3 View
Hello Dears, i know the purpose of isopropenol in DNA extraction, collect the DNA in the form of pellet from the sample. what is the reason and logic we use chilled isopropenol in the extraction...
12 December 2019 6,177 6 View
When precipitating DNA, in a dna extraction, I used sodium acetate 3M, in falf of my samples, and in the other half I used potassium acetate 5M, but both are precipitating in form of granules...
12 December 2019 7,709 4 View
12 December 2019 2,061 5 View
I’m working on a DNA extraction optimization for Ascaris, but we have no fecal samples that are positive for Ascaris. We have obtained some Ascaris eggs, and I will be spiking negative human stool...
12 December 2019 3,322 7 View
I am currently facing difficulty in the DNA extraction because of the viscosity of the sputum samples. I am using the Chelex based extraction method which does yield in DNA but it is inefficient...
12 December 2019 3,331 1 View
Dear experts, I observed the phenomenon that when we leave the blood agar plate for over 3 months, there was a change from red to extreme dark red ( or light black color). I would like to ask...
10 December 2019 6,593 4 View
I am developing a phylogeography workflow and I need PERMUT cpSSR to compute NST and GST for mitochondrial Cytochrome c Oxidase (COI) haplotypes so that I can test for genetic differentiation...
09 December 2019 764 2 View
I streaked pure culture on three different media : Mannitol Salt Agar (MSA), Malt Extract Agar (MEA) and Potato Dextrose Agar (PDA) media. There are colonies of bacteria grow on MSA, slightly...
23 November 2019 4,606 0 View
The blood I am using has been collected from Nile Crocodiles and is stored at -20°C.
21 November 2019 1,182 3 View
I have been differentiating multiple iPSC lines into NPCs and then into astrocytes. I would like to do some qPCR to further characterise my iPSCs and astrocytes but was wondering how best to...
20 November 2019 6,702 4 View
I extract DNA from various sludge samples according to the method by Griffiths et.al (2000). By now I did not have any problem, even in case of sludges which contain a great amount of silage and...
15 November 2019 2,638 3 View
Hello, We recently obtained some insect samples (Asphondylia) collected a few months ago by collaborators. The individuals were freeze dried, placed into pure ethanol, and shipped to us on dry...
11 November 2019 1,233 2 View
11 November 2019 8,364 3 View
11 November 2019 1,626 3 View
I'm looking into extracting DNA from buccal cells. I have no experience in using this source material and was wondering if there was a general consensus on preferred method? Yield would be a...
11 November 2019 9,268 3 View
I have some sporocarps of gilled mushrooms frozen at -20°C and I want to regenerate the mycelia from them. can it be used as inoculum? if YES, Please suggest me available methods to do so.
11 November 2019 6,215 5 View