- The Eppendorf tube was then placed in a microcentrifuge. Notice how it is balanced with another tube placed opposite it in the centrifuge drum to ensure smooth rotation.
Other information about the experiment:
- This method extracted a DNA sample that can be stored for use in further DNA lab work.
- The cells used in this experiment were human cheek cells. They were collected on a cotton bud (swab) by gently scraping the inside of the cheek. The swab was placed into an Eppendorf tube
- A lysis solution was added to burst the cells open. The lysis solution and cells were placed in a waterbath.
- The lysis solution contained detergent and an enzyme (a proteinkinase).
hi dear Chanel
It depends on your protocol and what are you trying to do. if you want just to eliminate cell debris, centrifugation at 500 x g for 5 minutes it's enough, after that you can do centrifugation at a higher speed 15000 x g until 20,000 x g for 15 min if you are interested for example in DNA or chromatin. but as I said all depends on what your aims are, you can search for a well-detailed protocol and follow the instruction.
That's depends on what you need to extrat! centrifuge speed of DNA extraction is different from another extraction!
Rashad Alakhras Cheek cells were used and DNA was separated from protein and cellular debris. DNA was extracted too.
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