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Questions related to DNA Extraction
We shall collect biopsies from skin/hoof of animals for bacteriological microbiome analysis. We need suggestions on DNA extraction kit that may be used for this. We prefer to keep the biopsies in...
25 September 2020 7,097 4 View
I will be extracting trypanosome DNA from mid-guts of tsetse flies for LAMP analysis. Kindly advice on protocol to adopt for maximum yield and benefits of Chelex method over the regular method....
24 September 2020 783 4 View
Hello, I've encountered a significant issue with Qubit 2.0 readings on DNA extracts of Arctic plant roots. The samples have all been run previously, and when double-checking them, the readings...
22 September 2020 6,528 0 View
Is there any way in which we can check whether the DNA inside the cell is negatively or positively supercoiled?
13 September 2020 9,077 2 View
Does anyone have experience of extracting DNA out of dairy cow's milk? I am interested in the DNA present in the B-cells. I have done extraction from blood but want to try extraction from milk. I...
03 September 2020 3,746 5 View
I have a large set of samples to extract DNA from, and am trying to plan out the most efficient way to process them. I have a few options, but the most efficienct would be to first process all of...
17 August 2020 3,065 9 View
I'm digesting a few different mammalian tissues with a spin kit and proteinase k. Some tissues (esp. testis and pituitary gland) look fully digested and clear. But once the lysis buffer is added,...
10 August 2020 4,671 2 View
Hi, I am trying to source a 96 well magnetic separation plate. Most of the examples I have found seem to have a limited number (24) of magnetic positions on a plate. Is there a reason there are...
10 August 2020 7,542 1 View
I'm extracting DNA from various bull tissues and there are some that don't work with a spin column kit, like brain tissue that contains a lot of lipids. For those, we do Phenol-chloroform...
06 August 2020 2,351 2 View
Hello, I want to extract DNA for ITS sequencing to identify unknown bacteria growing together with Dothistroma isolates. What kit may I use? Normally we use EZNA fungal and plant kits, it is...
31 July 2020 6,799 7 View
While running real.exe am getting the following error. Can anyone help me out. 01 2020-07-15_09:00:00 Turning off use of TROPOPAUSE level data in vertical interpolation Using sfcprs3 to compute...
17 July 2020 9,507 0 View
Have a Shimadzu GCMS QP10 GCMS system in a classroom lab. Is there a simple step to add to the method they use so the split ratio goes to 5 and saves gas? I'm mostly at home and don't have the...
10 July 2020 4,830 0 View
Due to the pandemic, I can't go back to my university to conduct experiments. I have my stool samples and extraction kit with me in a smaller laboratory however they don't have the equipment I...
08 July 2020 5,463 1 View
Hi all, it might be a very basic question, but I need some opinions: I need to separate a 294 bp wild type PCR product from a 232 bp knock-out PCR product. I want to do DNA extraction out of the...
09 June 2020 9,823 9 View
We have to select plasma or serum to collect in order to measure mRNA levels of certain genes.
29 May 2020 5,100 0 View
How we can improve CfDNA concentration through extraction?. Can we use Thermocycler or precipitation to concentrate CfDNA without damaging DNA integrity?.
20 May 2020 5,976 2 View
Hello, I need to collect blood plasma from animals for RNA extraction, and I want to add Qiazol and freeze quickly in dry ice to preserve the RNA. The problem is that in the procedure room where...
27 April 2020 8,356 0 View
Hello all, I am planning to subject bacterial cells to combinations of heat and mechanical lysis (bead-beating) and wanted to check for DNA shearing/integrity using gel electrophoresis. The DNA...
20 April 2020 1,855 0 View
Herbarium collections are increasingly becoming enormous resource for DNA studies; but for many species in certain families, there is difficulty in obtaining amplifiable DNA. I would like to...
26 March 2020 4,655 11 View
I have a two part question: (1) If DNA is extracted from a mammalian cell and the total quantity (yield) attained is significantly larger than what is theoretically expected considering the total...
26 March 2020 4,324 3 View
I will be using Axiom Genome-wide Human Origins 1 array. Is there a way to tell whether a sample is contaminated with DNA from another sample during the extraction stage (for example, excessive...
07 March 2020 8,167 3 View
Hi there, I am using Qiagen blood/tissue DNeasy kit on some heparinized fresh mouse blood (about 120ul) and I am getting only
03 March 2020 2,849 4 View
Recently I performed a DNA extraction using Qiagen DNeasy kit and the results show a 260/230 ratio of 1.05 likely due the presence of phenol or other organic contaminant... I have read that you...
17 February 2020 1,151 3 View
I'm doing my research on moss and right now i'm doing dna extraction from moss sample. I use the Qiagen Dneasy plant mini kit but i didn't get any result. I don't know what i do wrong. I thought...
06 February 2020 320 2 View