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Questions related to DNA Extraction
Have a Shimadzu GCMS QP10 GCMS system in a classroom lab. Is there a simple step to add to the method they use so the split ratio goes to 5 and saves gas? I'm mostly at home and don't have the...
10 July 2020 4,799 0 View
Due to the pandemic, I can't go back to my university to conduct experiments. I have my stool samples and extraction kit with me in a smaller laboratory however they don't have the equipment I...
08 July 2020 5,339 1 View
Hi all, it might be a very basic question, but I need some opinions: I need to separate a 294 bp wild type PCR product from a 232 bp knock-out PCR product. I want to do DNA extraction out of the...
09 June 2020 9,731 9 View
We have to select plasma or serum to collect in order to measure mRNA levels of certain genes.
29 May 2020 5,040 0 View
How we can improve CfDNA concentration through extraction?. Can we use Thermocycler or precipitation to concentrate CfDNA without damaging DNA integrity?.
20 May 2020 5,899 2 View
Hello, I need to collect blood plasma from animals for RNA extraction, and I want to add Qiazol and freeze quickly in dry ice to preserve the RNA. The problem is that in the procedure room where...
27 April 2020 8,289 0 View
Hello all, I am planning to subject bacterial cells to combinations of heat and mechanical lysis (bead-beating) and wanted to check for DNA shearing/integrity using gel electrophoresis. The DNA...
20 April 2020 1,820 0 View
Herbarium collections are increasingly becoming enormous resource for DNA studies; but for many species in certain families, there is difficulty in obtaining amplifiable DNA. I would like to...
26 March 2020 4,573 11 View
I have a two part question: (1) If DNA is extracted from a mammalian cell and the total quantity (yield) attained is significantly larger than what is theoretically expected considering the total...
26 March 2020 4,211 3 View
I will be using Axiom Genome-wide Human Origins 1 array. Is there a way to tell whether a sample is contaminated with DNA from another sample during the extraction stage (for example, excessive...
07 March 2020 8,038 3 View
Hi there, I am using Qiagen blood/tissue DNeasy kit on some heparinized fresh mouse blood (about 120ul) and I am getting only
03 March 2020 2,769 4 View
Recently I performed a DNA extraction using Qiagen DNeasy kit and the results show a 260/230 ratio of 1.05 likely due the presence of phenol or other organic contaminant... I have read that you...
17 February 2020 1,043 3 View
I'm doing my research on moss and right now i'm doing dna extraction from moss sample. I use the Qiagen Dneasy plant mini kit but i didn't get any result. I don't know what i do wrong. I thought...
06 February 2020 280 2 View
02 February 2020 1,570 2 View
I performed DNA extraction using the Quiagen Blood & Tissue Kit on a set of liver samples; and happened to find tissue in the final DNA extract (black precipitate at the bottom). I did add the...
02 February 2020 3,633 3 View
Dear all, I performed DNA extraction using Qiagen DNeasy Blood and Tissue Kit, followed the protocol with an addition of 2ul RNase A with incubation of 2 min at R.T after adding proteinase K....
02 February 2020 5,486 4 View
02 February 2020 7,134 3 View
Hello everyone, I would like you to please help me in how to generate the inputs for use in the BAPS. I have sequences from the IOC gene. Thank you!
11 January 2020 687 1 View
I am attempting to set up a QC laboratory to test viral samples isolated from insects. The test will include gel electrophoresis and qPCR analysis. What I want to know is what type of water should...
17 December 2019 829 5 View
I work the research about stabilization of soil by Biological soil crust especially algae (Cyanobacteria and green algae) Could anyone please help me about this topic soil stabilization using...
17 December 2019 756 2 View
I am doing a project for the evaluation of MinION-sequencing pipelines regarding MRSA and non-MRSA in terms of identification and resistance gene detection from cultured clinical isolates. My...
12 December 2019 8,116 3 View
Hello Dears, i know the purpose of isopropenol in DNA extraction, collect the DNA in the form of pellet from the sample. what is the reason and logic we use chilled isopropenol in the extraction...
12 December 2019 6,055 6 View
When precipitating DNA, in a dna extraction, I used sodium acetate 3M, in falf of my samples, and in the other half I used potassium acetate 5M, but both are precipitating in form of granules...
12 December 2019 7,668 4 View
12 December 2019 2,002 5 View