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Questions related to DNA Extraction
So we use Ethidium bromide to stain our agarose gels for dna extraction and analysis. The software in our gel doc is malfunctioning so we are unable to capture images. So now we are using our...
11 November 2018 9,341 7 View
I want to do DNA extraction from blood by following Protocol 6 Rapid Isolation of Mammalian DNA (Sambrook). One of the equipment is CH3COOK, but I have no CH3COOK in my laboratory and only...
11 November 2018 9,691 6 View
I'm already done quantifying the viral DNA using absolute quantification (qRTPCR). Can anyone teach me how to convert my data into copies/gram? Currently it is in copies/ul. Here are some...
11 November 2018 1,572 4 View
10 November 2018 3,703 3 View
Hi, I'm going to be using 0.1mm zirconia/silica beads to break apart my mycobacterial cells so I can extract DNA. Are there any tips for cleaning them? RPI says " beads can be soaked in a 1:10...
09 November 2018 6,444 1 View
04 November 2018 4,651 3 View
Looking expert opinion... I have collected marine sponge samples and were shadow dried for two weeks. Now the sponge samples are well dried and can be directly powdered by grinding. I would like...
10 October 2018 1,022 3 View
iwm looking for good protocol for dna extraction from nails clipping. does anyone can help? thanks, michal
10 October 2018 6,503 3 View
Several natural and human factors including geomorphic processes are consistently working to degrade the surface of the Earth and its landforms. It generally presumed that geological norm of an...
06 September 2018 3,140 0 View
I'm attempting to extract DNA from a single retrix from a small sparrow. I have upwards of 200 samples but only one feather per individual. The feathers have no visible blood spots on them. I've...
15 August 2018 5,450 2 View
I would like to know if someone has used it and what performance you have obtained compared to similar kits. Is it easy and fast as the product description says?
08 August 2018 5,223 0 View
08 August 2018 4,739 2 View
I found cytoplasmic male sterility (CMS) occurs in a potato variety while never observed in another closely related variety. Now I already got the mitochondrial genome data (around 800K each) of...
06 August 2018 265 5 View
I want to know What´s the best method for microbial DNA extraction of Theobroma cacao roots because i need microbial root DNA for metagenomic studies, I want to know if there is any suggestion...
07 July 2018 9,905 1 View
I'm currently preparing DNA from six populations of fish for whole genome sequencing on an Illumina HiSeq machine. Unfortunately half my populations have a low A260/A230 score (~1.6) indicating...
06 June 2018 5,271 4 View
Hello, I am trying to do DNA extraction from mites, for molecular analysis should i store the mites in 70% or 96% ethanol? Either way, later on when i want to crush the mites and add CTAB... do...
06 June 2018 2,556 2 View
Air sampled filter papers were analysed for chemical compostion using AAS. The results were in mg/kg. How do i convert mg/kg to ug/m3?
15 May 2018 9,008 11 View
I've successfully uploaded my metadata file and assembled, shotgun metagenomic dataet to my MG-RAST inbox. The metadata passed the QC-like step, my sequwnce data uploaded just fine and I could...
11 May 2018 5,622 7 View
11 May 2018 7,574 1 View
CURRENTLY AM DOING A RESEARCH FOCUSING ON TEA BREEDING USING MOLECULAR MARKERS FOR SPECIFIC TRAITS, I ALSO DO SAMPLE COLLECTION AND DNA EXTRACTION ON THE SAME
20 April 2018 5,546 3 View
04 April 2018 7,589 4 View
I am going to extract DNA from seawater bacterial. Firstly, I collect the seawater from beach, and then use 0.22 um filters for seawater filtration. I want to extract larger than 10kb DNA-reads...
04 April 2018 1,911 8 View
I want to know the artificial gene or strain which can be use as a DNA/RNA extraction control, and will lead you to RT PCR testing for any viral DNA or RNA.
04 April 2018 5,362 4 View
While working with DNA polymorphism analysis in DNAsp, I found some sequences were omitted from the analysis due to the presence of gaps (deletions) in some of the sequences as well as insertions....
02 April 2018 3,966 1 View