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Questions related to DNA Extraction
Hello, I am trying to do DNA extraction from mites, for molecular analysis should i store the mites in 70% or 96% ethanol? Either way, later on when i want to crush the mites and add CTAB... do...
06 June 2018 2,414 2 View
Air sampled filter papers were analysed for chemical compostion using AAS. The results were in mg/kg. How do i convert mg/kg to ug/m3?
15 May 2018 8,916 11 View
I've successfully uploaded my metadata file and assembled, shotgun metagenomic dataet to my MG-RAST inbox. The metadata passed the QC-like step, my sequwnce data uploaded just fine and I could...
11 May 2018 5,549 7 View
11 May 2018 7,516 1 View
CURRENTLY AM DOING A RESEARCH FOCUSING ON TEA BREEDING USING MOLECULAR MARKERS FOR SPECIFIC TRAITS, I ALSO DO SAMPLE COLLECTION AND DNA EXTRACTION ON THE SAME
20 April 2018 5,453 3 View
04 April 2018 7,504 4 View
I am going to extract DNA from seawater bacterial. Firstly, I collect the seawater from beach, and then use 0.22 um filters for seawater filtration. I want to extract larger than 10kb DNA-reads...
04 April 2018 1,812 8 View
I want to know the artificial gene or strain which can be use as a DNA/RNA extraction control, and will lead you to RT PCR testing for any viral DNA or RNA.
04 April 2018 5,255 4 View
While working with DNA polymorphism analysis in DNAsp, I found some sequences were omitted from the analysis due to the presence of gaps (deletions) in some of the sequences as well as insertions....
02 April 2018 3,884 1 View
I grinded callus for DNA extraction, but I couldn't make it into very fine powder, to how extent that would affect the extraction procedure
28 March 2018 4,204 3 View
Hello everyone I am trying to perform ChIP-seq experiments to find downstream effectors of a Transcription factor in Drosophila. However, I am having trouble with the sonication steps. Since my...
03 March 2018 2,862 4 View
Hi all, I'm trying to clean up some DNA for genomic sequencing (Nanopore and Illumina), but i'm still struggling with the purity of my DNA. My 260/280 ration is ok, that is not an issue, however...
03 March 2018 9,309 3 View
I have extracted DNA oligonucleotide of size 77 bp from a crushed 10% PAGE gel using 3 rounds of LiClO4 in water. The extracted elute was dried down in a vacuum centrifuge to a certain volume and...
03 March 2018 6,803 5 View
can anyone tell me about biocrust (algae and cyanobacteria) used for treatment soil contaminated with heavy metal such as (pb , cd) and how long biocrust can hold heavy metals
03 March 2018 4,668 3 View
Can anyone identify method to inoculation soil in algae or cyanobacteria to determine the affect biological soil crust on erosion by jet erosion test i want use this mold to grow soil crust on...
25 February 2018 605 2 View
Given 80 ug/ml XGAL stock, to add 40 ul AND given 100 mM IPTG, to use 10 ul
10 February 2018 6,629 1 View
1. 1.5ml of the bacterial culture was poured into the microcentrifuge tube and then centrifuged at five minutes at 10,000 rpm. 2. the supernatant was discarded and the pellet was left at the...
01 January 2018 3,395 3 View
During search in GenBank database I found in some sequences the following comment: GenBank staff is unable to verify sequence and/or annotation provided by the submitter. so, what are the reasons...
29 December 2017 3,227 6 View
Dear all, I performed phage DNA extraction by phenol/ chloroform followed the below steps: - Precipitation of phage particle using PEG8000 with the presence of NaCl - Add DNase, RNase to digest...
11 November 2017 3,025 3 View
Fresh plant tissue is recommended for DNA extraction while plant tissue frozen at -20 degree centigrade can produce poorer DNA output. I am interested to know how big the difference is when DNA...
11 November 2017 7,764 5 View
we are extracting protein from fruits. in nearly all buffers, CHAPS is used but i don't have this detergent.
31 October 2017 3,029 0 View
Gaining insight into the implications of the microbiome on human health requires not only a precise characterization of the microbial communities at the structural level, but also to generate...
26 October 2017 7,174 3 View
DNA extraction protocol using Chelex-100 is cheap and very useful technique. However, the protocol varies between lab to lab, or people to people. My lab is fungal taxonomy lab, so DNA is...
19 October 2017 8,244 1 View
Hello everyone, I am looking for some advice on the CTAB method for DNA extraction from faecal samples (from bat droppings). I am following the below written method but I am a bit unclear on the...
18 October 2017 5,549 1 View