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Questions related to DNA Extraction
I want to use the AllPrep PowerFecal DNA/RNA Kit (Qiagen) to co-isolate microbial DNA and RNA from human samples other than feces (such as saliva, skin, etc). Has anyone tried it with successful...
27 June 2017 6,537 1 View
Other colleagues are extracting good quality DNA of other crops with the same chemical. so all chemicals are working good.
24 June 2017 778 9 View
Could you please advise me any kits/methods to purify viral DNA from the lobster tissues. Our project aims to sequence whole genomes of several DNA virus species causing disease in lobsters. Size...
14 June 2017 7,868 2 View
06 June 2017 1,182 9 View
This DNA extraction is from a filter that has had lake water pass through it with the goal to capture microbial DNA on a .22 um filter.PEG 6000 is normally used for this protocol but PEG 8000 was...
06 June 2017 1,148 2 View
I'm planning to do extractions for metagenomics & metatranscriptomics of insect gut contents. Any other recommendation for quick isolation and separation of DNA and RNA from the same sample...
06 June 2017 7,128 1 View
Hi I will be cheerful if you share your experiences about extracting DNA from Insects (especially beetles) using Chelex 100 resin. If it's possible, please share the protocol and details that you...
06 June 2017 4,337 4 View
06 June 2017 1,653 0 View
May I know is there anybody dealing with DNA/RNA extraction from baby stool by using kits? Any advice in between QIAGEN vs Zymo? Or other non-kits related methods which you feel is workable and...
06 June 2017 920 4 View
I want to know which of these options apply during fieldwork.
06 June 2017 7,978 1 View
Dear all, for a rather big project, I need to homogenize 600+ samples. So far, 5mL Eppendorf tubes have proven to be the most ideal for sampling, as 2mL tubes are too small and 15mL falcon tubes...
06 June 2017 5,881 8 View
Hi, I am getting holes in the cap of my sample tubes containing root tissue when grinding them in a Retsch 400 mill. The mill is configured at 30 Hz for 1.5 min. Samples and adaptors are first...
06 June 2017 4,718 3 View
06 June 2017 7,353 2 View
I have extracted DNA from a vaccine containing aluminium hydroxide gel using sodium citrate (tribasic). The extraction has work well and I have a good qubit reading for dsDNA as well as a good...
06 June 2017 3,933 2 View
I am trying to extract fungal DNA from clinical samples by using Guanidine thiocynate- phenol saturated . I got good DNA quantity but poor 260/230 ratio. Whether repeatation of CI step can...
06 June 2017 3,658 2 View
hello do Whatman® FTA® cards preserve RNA of a sample? for example, I am using Whatman® FTA® cards to collect Blood and saliva samples and kept them for 1 month at room temperature.. is RNA preserved?
06 June 2017 1,021 1 View
I will be extracting DNA from mouse feces and lung tissue and would like to use a phenol/chloroform DNA extraction method in combination with a lysis step using CTAB and bead beating. It has been...
06 June 2017 3,334 2 View
I hope to extract the DNA of Lactic acid bacteria (LAB) for quantitative analysis. I have put lysozyme 50 ul (60 mg/ml), RNase 3 ul (1 mg/ml), 25% SDS (25 ul) and proteinase K (25 ul) after...
22 May 2017 1,691 4 View
Could somebody recommend a method for bacterial RNA extraction from human frozen tissue?
16 May 2017 6,140 3 View
Hi all, I am in the midst of extracting my snail tissue samples via Omega BioTek's EZNA Mollusc specific DNA extraction kit and I am having some shearing/degradation. I extracted two samples as...
15 May 2017 578 3 View
05 May 2017 3,047 4 View
As I am trying to extract the DNA from herbarium Fungi and some of them are really old, need some advice to get the best result? Thanks in advance.
05 May 2017 910 3 View
05 May 2017 2,091 3 View
05 May 2017 9,385 4 View