What's typical DNA yield and quality of DNeasy Powered Food Kit?

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Any papers related to the topic of extraction of optical properties of tissue-mimicking phantoms?

Dear Community, I'm trying to find some information about the extraction of optical properties of phantom without the usage of spectrophotometer. Thanks in advance

21 December 2020 6,770 3 View

How to interpret Generalised Linear Model output from SPSS?

I am trying to run an ordinal logistic regression using Generalised Linear Model from SPSS. There variables are A,B,C,D, and a moderation variable A*C. Variables A and D are continuous variables...

02 December 2020 5,159 3 View

How to isolate Clostridium from mixed culture?

My Clostridium tyrobutyricum seems not producing spores. I incubated the Clostridium tyrobutyricum in 10 ml of RCM at 28 degrees Celcius for 5 days anaerobically, then heat the whole tube in 75...

25 October 2020 3,386 1 View

Protocol for purifying proteins that are stable up to 65-70C by heat treatment?

We are trying to save up on reagents and heat treatment is a promising protocol, but there are limitations with the thermostability of strand displacing DNA polymerases. Does anyone know of a...

06 October 2020 4,708 4 View

Flow Cytometry help?

Dear All, With Flow cytometry, people would buy a specific antibody and it's associated isotype control. What i would like to ask is, if my antibody is mouse IgG2b, could i use the an isotype...

05 May 2020 9,595 4 View

Peptide concentration for stimulating murine CD4+ and CD8+ splenocytes?

I'm trying to figure out a good starting concentration of 15mer peptides to stimulate mouse CD4+ and CD8+ splenocytes for cytokine staining. We are pooling 72 peptides for one of our conditions to...

17 October 2019 6,974 2 View

Does cryotherapy immediately after skeletal muscle injury improve or hinder muscle healing and regeneration?

Does anyone have more information on journal articles regarding this topic? I have found 3 studies with opposing conclusions. Ramos et al. (2016) and Singh et al. (2017) both suggest that...

17 September 2019 2,378 3 View

How long does an IAC antibody-coupled NHS column last?

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17 January 2019 5,664 4 View

Why T cell can't proliferation?

Hello everyone, I use sorting to get T cells(CD4 and CD45RB double positive) and then culture in coating CD3(10ug/ml) U buttom 96 plate and treat the cell with soluble CD28(4ug/ml). After 3 days,...

11 March 2018 3,190 3 View

Methods for ApoB48 western blotfrom density gradient fractions?

I have cultured primary enterocytes with lipids. After then I collected the medium and cells' microsome lumenal proteins for NaBr gradient (d=1.006~1.10), and each fraction (1ml) was collected...

30 July 2017 7,697 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Calculating volume of DNA required from DNA concentration?

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26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

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25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

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25 February 2021 1,660 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

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25 February 2021 9,761 1 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Lim Wei Gene

You can try adding lysozyme to help in breaking down the cell wall of gram-positive bacteria before proceeding in DNA extraction.

To Chiu

I treated the pallet from the sample with 0.06 g/ml lysozyme in 400mM TE/EDTA buffer before the kit, but the yield and quality still low. The PCR result improved, though.

Chinaza Godswill Awuchi

Kindly check https://www.qiagen.com/us/resources/download.aspx?id=55456cd5-2de4-43b2-8eaa-760d1371710a&lang=en