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Questions related to DNA Extraction
I want to extract cp DNA from some desert plant, i have issue in extraction of cp DNA i am following An Improved Chloroplast DNA Extraction Procedure . After second centrifugation 3500G i get...
10 October 2017 9,220 3 View
DNA extraction protocol using Chelex-100 is cheap and very useful technique. However, the protocol varies between lab to lab, or people to people. My lab is fungal taxonomy lab, so DNA is...
10 October 2017 5,425 1 View
Hello everyone, I am looking for some advice on the CTAB method for DNA extraction from faecal samples (from bat droppings). I am following the below written method but I am a bit unclear on the...
10 October 2017 6,624 1 View
I want to perform clean up on my digests but currently out of ERC from minElute clean up kit. Can I substitute it with buffer QG ? These are all from qiagen. Somebody told me I could do this but...
05 October 2017 8,297 3 View
I want to confirm my E. coli DH5alpha stock before transformation procedure. I have already inoculated it to the EMB agar, but there was no growth. But, I'm not sure about my EMB agar stock, so i...
28 September 2017 9,454 1 View
Hi, I'm working on metagenomic analysis of undefined mixed culture of algae-bacteria culture using 16s/18s rRNA analysis using Illumina sequencer. Any suggestion for the best kit to do total DNA...
16 August 2017 738 2 View
08 August 2017 3,912 2 View
i recently ran a DNA extraction and isolation experiment on an an indigenous plant but no bands were observed on the agarose gel. What could be the reason for not obtaining DNA even though...
08 August 2017 8,007 0 View
Hi everyone, I am currently running a multiplex PCR, and product size varies from 120 bp to 500 bp. I have checked all those temperature on thermo machine, which are fine according to the paper I...
26 July 2017 9,756 4 View
I want to use the SpeedVac concentrator to concentrate some gDNA samples i have extracted from a co-culture experiment. The samples contain both prokaryotic and eukaryotic DNA. Eventually only the...
25 July 2017 6,718 2 View
Hi, I have a primer which produces multiple amplicons, so i observe multiple bands on gel. I was expecting that, and i would like to cut the bands, extract DNA, and use it as template for another...
19 July 2017 2,106 38 View
How can extract DNA genomic with high purity in fungal with the CTAB method?
17 July 2017 8,601 6 View
I am looking for a protocol for DNA extraction from bucal swabs or saliva but most of them use kits and I only have reactives for traditional methods.
16 July 2017 6,580 5 View
Hello all. I have used consensus PG/MY primers for HPV typing and the PCR was standardized with HPV positive control. But suddenly I am getting smears instead of bands though I used same...
12 July 2017 8,663 3 View
As I said here: https://www.researchgate.net/post/Looking_for_a_DNA_extraction_protocol_from_adult_beetles_Coleoptera I finally extracted the DNA from adult beetles using a CTAB protocol. But the...
07 July 2017 1,543 8 View
Please provide me practically viable solution.
07 July 2017 6,444 6 View
I want to extract RNA from fish blood for making cDNA. What would be suitable temperature for preserving the blood sample in case of delayed extraction of RNA from the blood? Does RNA degrade if I...
07 July 2017 9,069 5 View
Can any one provide the standardized protocol for Gnomic DNA isolation from aerobic bacteria. I am doing with Kit method and its too cost so i want to isolate DNA with manual methods
07 July 2017 240 4 View
I need to replicate the results obtained with this lysis buffer, but manufacturing it myself would allow me to lower costs. I have read the SDS, I know it has guanidinium thiocyanate and EDTA, but...
07 July 2017 3,449 0 View
We would like to extract DNA from whole blood using the Prepman Ultra Sample Reagent but we cannot find a protocol to do it. Could you help us?Thank you!
07 July 2017 4,217 22 View
I want to avoid ethanol as it is known to hinder downstream amplification process. Also, I am in a process of developing an automated diagnosis of ZIKA and would want to avoid...
07 July 2017 5,393 3 View
I'm not sure if research gate allows a this type of questions but it's worth trying. Dear community can someone help me with an online version of the following research article: Doyle J. J,...
07 July 2017 8,372 3 View
My student has bundles of bee-collected pollen from a phenology study, and would like to get DNA barcode data from them. We have tried following protocols involving liquid N2, and grinding 0.1g of...
07 July 2017 1,090 3 View
07 July 2017 6,131 2 View