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Questions related to DNA Extraction
I grinded callus for DNA extraction, but I couldn't make it into very fine powder, to how extent that would affect the extraction procedure
28 March 2018 4,267 3 View
Hello everyone I am trying to perform ChIP-seq experiments to find downstream effectors of a Transcription factor in Drosophila. However, I am having trouble with the sonication steps. Since my...
03 March 2018 2,917 4 View
Hi all, I'm trying to clean up some DNA for genomic sequencing (Nanopore and Illumina), but i'm still struggling with the purity of my DNA. My 260/280 ration is ok, that is not an issue, however...
03 March 2018 9,357 3 View
I have extracted DNA oligonucleotide of size 77 bp from a crushed 10% PAGE gel using 3 rounds of LiClO4 in water. The extracted elute was dried down in a vacuum centrifuge to a certain volume and...
03 March 2018 6,852 5 View
can anyone tell me about biocrust (algae and cyanobacteria) used for treatment soil contaminated with heavy metal such as (pb , cd) and how long biocrust can hold heavy metals
03 March 2018 4,717 3 View
Can anyone identify method to inoculation soil in algae or cyanobacteria to determine the affect biological soil crust on erosion by jet erosion test i want use this mold to grow soil crust on...
25 February 2018 637 2 View
Given 80 ug/ml XGAL stock, to add 40 ul AND given 100 mM IPTG, to use 10 ul
10 February 2018 6,683 1 View
1. 1.5ml of the bacterial culture was poured into the microcentrifuge tube and then centrifuged at five minutes at 10,000 rpm. 2. the supernatant was discarded and the pellet was left at the...
01 January 2018 3,463 3 View
During search in GenBank database I found in some sequences the following comment: GenBank staff is unable to verify sequence and/or annotation provided by the submitter. so, what are the reasons...
29 December 2017 3,292 6 View
Dear all, I performed phage DNA extraction by phenol/ chloroform followed the below steps: - Precipitation of phage particle using PEG8000 with the presence of NaCl - Add DNase, RNase to digest...
11 November 2017 3,062 3 View
Fresh plant tissue is recommended for DNA extraction while plant tissue frozen at -20 degree centigrade can produce poorer DNA output. I am interested to know how big the difference is when DNA...
11 November 2017 7,820 5 View
we are extracting protein from fruits. in nearly all buffers, CHAPS is used but i don't have this detergent.
31 October 2017 3,088 0 View
Gaining insight into the implications of the microbiome on human health requires not only a precise characterization of the microbial communities at the structural level, but also to generate...
26 October 2017 7,213 3 View
DNA extraction protocol using Chelex-100 is cheap and very useful technique. However, the protocol varies between lab to lab, or people to people. My lab is fungal taxonomy lab, so DNA is...
19 October 2017 8,287 1 View
Hello everyone, I am looking for some advice on the CTAB method for DNA extraction from faecal samples (from bat droppings). I am following the below written method but I am a bit unclear on the...
18 October 2017 5,588 1 View
I want to extract cp DNA from some desert plant, i have issue in extraction of cp DNA i am following An Improved Chloroplast DNA Extraction Procedure . After second centrifugation 3500G i get...
10 October 2017 9,254 3 View
10 October 2017 5,455 1 View
10 October 2017 6,670 1 View
I want to perform clean up on my digests but currently out of ERC from minElute clean up kit. Can I substitute it with buffer QG ? These are all from qiagen. Somebody told me I could do this but...
05 October 2017 8,358 3 View
I want to confirm my E. coli DH5alpha stock before transformation procedure. I have already inoculated it to the EMB agar, but there was no growth. But, I'm not sure about my EMB agar stock, so i...
28 September 2017 9,492 1 View
Hi, I'm working on metagenomic analysis of undefined mixed culture of algae-bacteria culture using 16s/18s rRNA analysis using Illumina sequencer. Any suggestion for the best kit to do total DNA...
16 August 2017 790 2 View
08 August 2017 3,943 2 View
i recently ran a DNA extraction and isolation experiment on an an indigenous plant but no bands were observed on the agarose gel. What could be the reason for not obtaining DNA even though...
08 August 2017 8,048 0 View
all the manual or tips about LAMP think that false positive will hapen if you open the PCR tube for gel electrophoresis detection. How can it been for the enzyme has been inactived by 85 degree...
27 July 2017 7,282 1 View