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Questions related to DNA Extraction
I'm digesting a few different mammalian tissues with a spin kit and proteinase k. Some tissues (esp. testis and pituitary gland) look fully digested and clear. But once the lysis buffer is added,...
10 August 2020 4,544 2 View
Hi, I am trying to source a 96 well magnetic separation plate. Most of the examples I have found seem to have a limited number (24) of magnetic positions on a plate. Is there a reason there are...
10 August 2020 7,479 1 View
I'm extracting DNA from various bull tissues and there are some that don't work with a spin column kit, like brain tissue that contains a lot of lipids. For those, we do Phenol-chloroform...
06 August 2020 2,238 2 View
Hello, I want to extract DNA for ITS sequencing to identify unknown bacteria growing together with Dothistroma isolates. What kit may I use? Normally we use EZNA fungal and plant kits, it is...
31 July 2020 6,671 7 View
Due to the pandemic, I can't go back to my university to conduct experiments. I have my stool samples and extraction kit with me in a smaller laboratory however they don't have the equipment I...
08 July 2020 5,314 1 View
Hi all, it might be a very basic question, but I need some opinions: I need to separate a 294 bp wild type PCR product from a 232 bp knock-out PCR product. I want to do DNA extraction out of the...
09 June 2020 9,703 9 View
How we can improve CfDNA concentration through extraction?. Can we use Thermocycler or precipitation to concentrate CfDNA without damaging DNA integrity?.
20 May 2020 5,882 2 View
Hello all, I am planning to subject bacterial cells to combinations of heat and mechanical lysis (bead-beating) and wanted to check for DNA shearing/integrity using gel electrophoresis. The DNA...
20 April 2020 1,810 0 View
Herbarium collections are increasingly becoming enormous resource for DNA studies; but for many species in certain families, there is difficulty in obtaining amplifiable DNA. I would like to...
26 March 2020 4,553 11 View
I have a two part question: (1) If DNA is extracted from a mammalian cell and the total quantity (yield) attained is significantly larger than what is theoretically expected considering the total...
26 March 2020 4,186 3 View
I will be using Axiom Genome-wide Human Origins 1 array. Is there a way to tell whether a sample is contaminated with DNA from another sample during the extraction stage (for example, excessive...
07 March 2020 8,015 3 View
Hi there, I am using Qiagen blood/tissue DNeasy kit on some heparinized fresh mouse blood (about 120ul) and I am getting only
03 March 2020 2,748 4 View
Recently I performed a DNA extraction using Qiagen DNeasy kit and the results show a 260/230 ratio of 1.05 likely due the presence of phenol or other organic contaminant... I have read that you...
17 February 2020 1,013 3 View
I'm doing my research on moss and right now i'm doing dna extraction from moss sample. I use the Qiagen Dneasy plant mini kit but i didn't get any result. I don't know what i do wrong. I thought...
06 February 2020 263 2 View
02 February 2020 1,556 2 View
I performed DNA extraction using the Quiagen Blood & Tissue Kit on a set of liver samples; and happened to find tissue in the final DNA extract (black precipitate at the bottom). I did add the...
02 February 2020 3,617 3 View
Dear all, I performed DNA extraction using Qiagen DNeasy Blood and Tissue Kit, followed the protocol with an addition of 2ul RNase A with incubation of 2 min at R.T after adding proteinase K....
02 February 2020 5,465 4 View
02 February 2020 7,117 3 View
I am attempting to set up a QC laboratory to test viral samples isolated from insects. The test will include gel electrophoresis and qPCR analysis. What I want to know is what type of water should...
17 December 2019 799 5 View
I am doing a project for the evaluation of MinION-sequencing pipelines regarding MRSA and non-MRSA in terms of identification and resistance gene detection from cultured clinical isolates. My...
12 December 2019 8,103 3 View
Hello Dears, i know the purpose of isopropenol in DNA extraction, collect the DNA in the form of pellet from the sample. what is the reason and logic we use chilled isopropenol in the extraction...
12 December 2019 6,030 6 View
When precipitating DNA, in a dna extraction, I used sodium acetate 3M, in falf of my samples, and in the other half I used potassium acetate 5M, but both are precipitating in form of granules...
12 December 2019 7,660 4 View
12 December 2019 1,988 5 View
I’m working on a DNA extraction optimization for Ascaris, but we have no fecal samples that are positive for Ascaris. We have obtained some Ascaris eggs, and I will be spiking negative human stool...
12 December 2019 3,277 7 View