Elution of an antibody on a peptide immobilized column did not work?

I am very disappointed. I coupled a peptide with an N-terminal cysteine on a sulfolink column. HPLC shows that the peptide immobilization was almost complete. After cyclisation of the serum the...

25 May 2020 1,265 8 View

Hi! Can anyone show me how to calculate the total anthocyanins content using the OD at 545, 535 and 515 nm?

27 January 2017 7,419 3 View

Would you suggest any software executing ANN (MLP type) where connections between neurons (in consecutive layers) can be separately defined by user?

10 October 2016 3,464 7 View

What are the specific possible biases involved in having one coder in a qualitative analysis?

27 June 2016 998 4 View

Is there a reference article documenting the preferred imaging modes in oncology?

I would like citing a reference in a manuscript about the most used imaging modalities in oncology. Did somebody could provide me with a pointer?

17 August 2015 5,791 3 View

What is a clear definition for radiomics?

I am looking for a clear definition of "Radiomic". According to different papers from: Kuo, 2007; Lambin. 2012; aerts. 2014; singanamalli, 2014 and some others, I am not sure if the concept of...

15 February 2015 6,726 8 View

How can I compute the limit of agreement with Bland Altman featuring heteroscedasticity?

I am analysing repeated measurement of ratio-scaled data. For doing that I rely on Bland Altman analysis. The common case where Limit of Agreement is horizontal can be adressed by most of the...

20 February 2014 4,709 2 View

How to manage Bland Altman analysis with more than one couple of measurements?

I consider three readers circularly involved in Bland-Altman 2-by-2; threfore I obtained 3 Bland-Altman analysis with corresponding Limit of Agreement (and Confidence Interval). In order to get...

16 January 2014 9,164 6 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View