I have a compound stock diluted in DMSO. What would be a good way to give it to mice by oral gavage? What concentration of DMSO could be tolerable for mice if administered by oral gavage ?
Thank you!
https://www.researchgate.net/post/Can_anyone_suggest_how_much_DMSO_can_be_given_to_mouse_by_oral_gavage_without_causing_any_harm#:~:text=The%20oral%20LD50%20for%20DMSO,DMSO%20with%20water%20if%20possible.
DMSO too is toxic, try to subtract the effect of DMSO from your results.
Hi Evgenii,
Before we can answer well, I think you should clarify how much of the compound you need to gavage (to determine the amount of DMSO the mice would get exposed to). Generally, you can gavage up to 1% of the mouse body weight by volume (so for a 20g mouse that would be 0,2 milliliter). Since it's difficult to gavage tiny volumes, you might need to dissolve your compound further in another carrier solution. Is the compound diluted in DMSO also soluble in other solvents?
Kind regards,
Jasper
Hi Jasper,
Thanks for the questions.
So the gavage volume would be 200-300 ul. The compound is moderately soluble in PBS: I used it diluted as 5%DMSO/5% Solutol in PBS for the other experiments before. I actually plan to do similar preparation for gavage, but will try to go down with DMSO to 2.5% and probably will have to increase solutol up to 10% to keep the compound soluble. That's my plan.
Just wondering whether someoned tried something like this before and whether it didn't cause the toxicity. I've seen people mentioned LD50 around 7ml/kg for mice, meaning about 140 ul. So, 5 ul DMSO for gavage should be OK. Just trying to confirm that with someone who did similar treatments.
Thank you!
Hi!! I’ve worked with many compounds for in vivo gavage and usually those soluble in dmso are also soluble in tween 80% + PBS, which is a better alternative to DMSO. You would first emulsify your compound in a small amount of tween 80 and then slowly add PBS until reaching the final volume. 1% tween 80 is not toxic. So if you have 10 mice, 200ul solution/animal, you will need 2000ul PBS and could use up to 20ul of tween 80. Use the same volume of tween 80 + PBS as your vehicle control. Of course, this is assuming you don’t have a huge amount of solid to start with.
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