How do I deal with small sample size and nonnormality in SEMs (AMOS or R)?

Hello! I have a sample size of N = 70 at the moment, hoping to aproach 100. My Model has 21 parameters to be estimated. The Variables are all not normally distributed. Now the options that I know...

20 July 2020 9,849 6 View

What is the Full Spectrum of Neuroscience Research Techniques that Exist?

Hi! I graduated with a Neuroscience and Psychology BSc in 2016, and my final year project was a bench-lab in vitro study of omega-3 oils applied to fluorescently-stained cortical neurons. I...

26 May 2020 4,650 6 View

Which cell culture inserts do you use?

In our experiment we use TC treated 96-well plates from Eppendorf and coat our protein of interest. After coating we add monocytes and get our readout. Now, we want to add activated T cells to the...

02 March 2020 6,065 2 View

What is the most efficient way to exchange buffer in protein soltuions?

Hy everyone, I'm facing some troubles when changing the buffer of my protein solution. I'm doing a 2-step purification of my protein, first on a nickel-column and second on a heparin column....

31 October 2019 793 4 View

Why does dynamic surface tension of gelatine solution increase before it decreases?

02 December 2018 949 3 View

Why are there a huge number of dead cells in my culture ?

Hello, I am culturing 293 cells for onward transfection. Unfortunately, I have observed that the cells are not multiplying as fast as they used to. Besides, i see several dead cells in the T25...

26 June 2018 4,274 5 View

Why does my SDS PAGE gel bubble and form a bell shape towards the bottom of the tank?

Hello, I am doing some SDS PAGE. Now, i am baffled by the fact that my gel bubbles at the bottom and there is a bell shape in the sample under separation, towards the bottom of the gel ( as...

15 May 2018 8,675 2 View

What is the best method to assess viability for adherent breast cancer cell lines?

For my project, I need to set up a viability method to see if inhibitors are responding. We currently don't know which method suits best and because there are so many we don't know which one we...

04 April 2018 2,737 3 View

What protocol could be implemented in analyzing for Total hydrocarbon content of soil using UV-Vis Spectrophotometer?

I need to determine the THC of crude oil polluted soil.

15 March 2018 7,557 6 View

Why is tubulin band not similar in all the well?

Hi every one, My PI and i are performing Western blot on B Amyloid protein. Unfortunately, after developing the Membrane, the control protein we are using (Tubulin) does not appear to have...

02 January 2018 939 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View