Replacement of MTT OR TTC?

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Is that any simple techniques to determine milk protein without kjeldahl method.????

Hello, Scintissist. I'm looking for a quick and easy way to detect protein or nirogen levels in milk without using the Kjeldahl method, so do let me know if you know of any.

11 May 2023 592 2 View

Does MALDI TOF detect and identify bacteriocin types from Cell free Supernatant?

Hello there, dear scholars. Allow me to present my challenges. Is MALDI-TOF capable of detecting and identifying bacteriocins such as nisin and pediocin? THANK YOU SO MUCH!!!

27 March 2023 7,901 1 View

MRS inhibitor for fungi?

Does the benzoic acid used as antifungal agents on to MRS agar?

18 March 2023 3,188 1 View

Discourse analysis and content analysis, how do I use both together to analyse data from two sources?

Currently, I am writing my article based on data extracts from teachers' interviews and video recorded classroom interactions. I developed themes and categories of both data inductively. I am...

13 March 2023 3,831 16 View

What is the impact of culture in nation building?

Cultural management is believed to be one of the main factors that determines the success of nation building.

05 March 2023 5,941 4 View

Bacterial species identification methods?

Hello Dear scientists, Please would you suggest me an idea on bacterial species identification to genus level, hence due to some financial shortage, i couldn't proceed to bacterial sequencing...

07 January 2023 3,795 3 View

What is an acceptable ratio of treated-to-control sample size in a Propensity Score Matching based impact evaluation?

It is intuitive to me that in a 1:1 proportion of treatment: control groups sample size would be problematic if a treatment observation fails to have a corresponding match from the control...

19 November 2021 5,524 2 View

What is the best transport media for anaerobic bacteria (lactic acid bacterai)?

I need to transport lactic acid bacteria using anaerobic transport media like carl Blair and Thioglycolate broth , so rather than this media or other else what is the best appropriate...

17 October 2021 6,605 3 View

Any formula or method to calculate yield loss caused by each plant diseases when more than one diseases occurred simultaneously on a plot/genotype?

In plant pathology experiments, some times multiple diseases can occur on a single plot even on a single plant at the same time and growth stage of the crop. Finally it becomes difficult to...

21 September 2021 4,026 4 View

What is a free market or market economy?

Market economy is one of the economic models which is mainly applied in the West. There are definitely differences among the market economy model and the other economic models. It is thus...

02 January 2021 7,577 27 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,105 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 639 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,724 2 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,503 2 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 192 4 View

Assistance with MTT Assay Data ?

I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...

26 July 2024 7,511 3 View

Can I use sonicator only to lyse the exosomes and measure their protein contents using BCA assay kit?

Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...

23 July 2024 4,603 4 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

My question concerns MTT cell viability assay?

Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...

21 July 2024 9,911 1 View