How these solvents limit shrinking ice crystals during freeze-drying or vitrification? and what are the main differences between DMSO and Glycerol? where each one is useful?
I will be grateful if anyone answers these questions.
Hello Neda Jalilian,
For questions about Cryopreservation and Freeze-Drying of Cells and Tissues you can read this book :
Chapter Principles Underlying Cryopreservation and Freeze-Drying of ...
For you interrogation about DMSO and Glycerol, feel free to browse the following RG topic :
https://www.researchgate.net/post/Why_is_DMSO_rather_than_Glycerol_used_while_preparing_cells_for_cryo_and_why_is_glycerol_used_preferentially_while_storing_microbial_culture
Hoping that this comment will be useful. Feel free to discuss or share some informations about your cryopreservation studies.
Ludovic Dumont, Ph.D.
I want to simulate two different ceramic structures. Each of the structures has a different number of atoms. I divided my simulation box into two parts. Now, in the create_atom section, I don't...
08 May 2024 9,647 2 View
Hi there, I have some technical questions during the procedure of GraphVar for my EEG data.I would be happy if anyone can help me with "GraphVar". Thank you in advance for your help. Best, Neda
17 August 2023 821 0 View
Hi everyone, In the GUI, variable section, I created the variable sheet and I encounter an error while I execute it. I have attached screenshots of them. Any help would be really appreciated. Neda
16 August 2023 3,920 2 View
Hi everyone, I am working on EEG data and want to find functional connectivity and finally the graph. Would you please help me with which toolboxes are more useful to do this work? GraphVar or...
15 August 2023 6,837 2 View
I want to enrich the semen and soap vaginal sample in mycoplasma culture medium with horse serum, arginine and phenol red. But I have a problem with how to cultivate. After consuming arginine in...
27 July 2023 2,439 0 View
In research to investigate the expression of genes in breast cancer, which of the housekeeping genes is the best gene for QRT-PCR؟
21 July 2023 3,158 1 View
Hello I electrospun PVA and then heat-treated it. Now, after the heat treatment, the fibers stick to the foil and do not separate. Do you have a suggestion for removing PVA from the foil?
05 July 2023 1,963 3 View
The mathematical function of TPMS unit cell is as follows: (for example Gyroid) sin x * cos y+ sin y * cos z+ sin z * cos x = c parameter 𝑐 determines the relative density of the unit cell. I am...
29 March 2023 2,529 2 View
I stained one cryovial PBMCs according to the CFSE protocol (invitrogen C34570). One day before staining, I thawed the cells and rested them in media (ingredients is below ) for 24 hours. On the...
01 March 2023 4,658 2 View
Hi For hydrophobic drugs, we cannot do drug loading and release in the PBS, and have to use non-aqueous solvents. So this condition is far from the in vivo. What solution do you suggest to solve...
22 February 2023 2,939 3 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,686 1 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Do these peaks in the curve indicate primer dimer or non-specific products?
01 August 2024 7,466 0 View
What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?
01 August 2024 1,722 1 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
Hi researchers! I'm working on soil texture analysis, and the end result for sand is doubtful because there is black sediment appearing after drying, as shown in the figure. Is it considered sand?...
30 July 2024 557 2 View
I am doing research on wear mechanism of the additive manufactured metal parts under dry condition. Please share the reference to start the analytical modelling of the wear prediction.
29 July 2024 6,115 0 View
I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...
22 July 2024 6,592 3 View
I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...
19 July 2024 8,522 0 View