I would be very grateful for any help regarding my problem.
My research requires applying betulin (and betulinic acid) solution on the cerebral cortex slices of rats. With the stock solution of 20mM, I could not obtain any dilutions as both 2mM and 1mM became flocculent and hazy.
I vortexed them thoroughly and later they were kept in 37°C water bath for over an hour, with literally no improvement.
Literature data mentioned researches done with 10mM, 20mM or even 100mM stock solutions of BE and BA, so, I suppose, successful dissolving is possible, yet I have no idea what else to try.
If anyone had ever done betulin in DMSO solutions, I would strongly admire any help.
No difference did it make when pH was changed; aquiring more concentrated stock solution and then dissolving it in water was also in vain; dissolving in 50% or 40% ethanol did not work out as well.
For now, I used the 1mM betulin solution to obtain the final concentration of 0,25mM, all in DMSO. Soon I will find out if it was enough.
I have prepared 10mM stock solution of betulin and betulinic acid in DMSO. It was the highest concentration possible to make. However, the compounds required dissolving for the long time (even 1hour) with occasional vortexing and warming at 37 Celcius degrees to obtain lucid solution. The stock was further diluted up to 1uM and lower in cell culture medium. In this, there were no problems with solution clarity (stock solution was dissolved substantially).
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