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Questions related to Sequencing
i have ITS and Rbcl sequences and i want to analyse for combination of markers. can anyone suggest me how to do
21 May 2017 4,770 1 View
For instance, how we generate an accurate results of detecting occlusion in a sequence of images in real time ?
20 May 2017 5,682 1 View
The m6A (N6-Methyladenosine) modification on RNA has a RRACH motif, so what about the 6mA (N6-Methyladenine) modification on DNA? Is there any specific sequence found though mapping its...
19 May 2017 682 2 View
The result in ncbi blast was NO SIGNIFICANT SIMILARITY FOUND
18 May 2017 4,184 1 View
Hi, I have been trying to sequence a mouse antibody but I get a +1 frameshift at the CDR3 junction for both light and heavy chains. Does anybody know what is the right sequence and why it does...
18 May 2017 8,883 1 View
Hello everyone, I have got sequences of the gene I have been working on and its two paralogs. As we know that they are expressed into the plant so I want to know the sequence similarity between...
15 May 2017 607 6 View
Hello, everyone: Recently, I was trying to build 3D structure of one protein which has no crystal structure, I was wondering some questions. First, what are the lowest threshold of sequence...
15 May 2017 7,888 2 View
gene bank ID is (L43921) but I am facing trouble while downloading the exact sequence i am looking for? The length of the desired gene sequence is 900 bp, anyone who can paste the sequence and...
12 May 2017 1,959 2 View
Hi everyone! I am using a 2-step targeted amplicon approach for my Illumina library preparation and I wonder what's the purpose of the second part of the Nextera adaptors? The entire sequences...
11 May 2017 8,265 6 View
(1) How can we find the partial sum of n1000 instantly ? (2) is there is a simple method to find partial sum of the sequence f(n) ? (3) Any general method to compute...
07 May 2017 7,404 3 View
I want to design a single primer pair for a particular genus, involving its 5 species, so that while performing PCR, we involve a single primer pair, instead of 6 primer pairs.
06 May 2017 8,756 2 View
Hello, I am trying to find the best way to deal with PCR duplicates in ddRAD data. I was wondering if there is a reliable way to identify and exclude them in a dataset that has been produced...
02 May 2017 10,000 3 View
In case p≥1, it is a classical result on convolution (discrete Young inequality)
01 May 2017 975 1 View
Our lab has sent rat cardiac tissue for sequencing and have obtained indigestible fastq data files. Is there a software I can use to organize these fastq sequence files in order to...
28 April 2017 7,878 3 View
Is size of over 400bp too big?
27 April 2017 3,652 2 View
Helle!I need to verify my miRNA microarray results with qPCR, and I have Agilent kit for RT-qPCR miRNA 1st-strand cDNA synthesis kit (#600036) and miRNA qPCR kit (#600583). It has some kind of...
26 April 2017 5,705 1 View
Hi, just want to know your experiences. Gene synthesis companies and also addgene start to use NGS for sequence validation. thus it seems that prices may have dropped to a degree where it becomes...
20 April 2017 2,265 6 View
Is there a way to annotate features in Serial Cloner that share sequence, such as a primer and a fragment of inserted genomic DNA carrying a CDS? This is possible in MapDraw (Lasergene), but...
20 April 2017 6,095 1 View
Hi all, Is there any new and reliable mini sequencer that is recommended?
17 April 2017 2,865 3 View
we have performed assembly using both CLC work bench (average length: 674 bp; N50: 890 bp) and TRINITY (average length: 725 bp; N50: 928 bp) and performed the analysis from both of them...
17 April 2017 6,842 4 View
I'm seeing a second call inside most peaks of my DNA sequencing data. This second (smaller) peak is exactly the next base called. Has any one seen this before? Is this a problem with primers? I'm...
17 April 2017 7,380 11 View
Hi there, I have recently assembled a NGS HiSeq data with ABySS 2.02. With the default setting (K-mers=64) I had a 183nt stretch of N (or Gap). I have changed k-mer to 40 and the gap reduced to...
15 April 2017 968 3 View
can anyone plese let me know how to clean phage contaminated plasmid.
12 April 2017 3,906 4 View
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09 April 2017 8,774 1 View