TATA Box in fungi - FAQS.TIPS

More Olumuyiwa Igbalajobi's questions See All

Is Landsat 8 Collection 2 Level 2 Rescaled Thermal Band (Surface Temperature in Kelvin) the same as Land Surface Temperature (LST)?

I have Landsat 8 Collection 2 Level 2 which is in Surface Reflectance and Surface Temperature (Thermal Band). After rescaling with one of the two methods highlighted below, Can I say the Surface...

22 February 2023 2,393 2 View

Double joint PCR for 6kb?

I have been trying to join 3 fragments (3kb, 2kb and 1kb respectively) in the last days using the DJ PCR protocol but to no avail. My 2nd PCR annealing temperature was at 57°degree for 10 min and...

30 July 2020 1,749 2 View

What are the most common comorbidity of COVID-19?

Coronavirus with respect to other infections.

02 May 2020 5,303 3 View

Are you considering how the alternative feed resources can optimize the reproductive functions of livestock?

Are you considering how the alternative feed resources can optimize the reproductive functions of these livestock?

15 January 2018 2,394 3 View

What is the influence of television models on children's risk- taking behaviour?

Are children's risk-taking behaviours a consequence of their attention to television programmes?

30 March 2016 3,405 3 View

How do I transform for Aspergillus fumigatus?

For months now i have been trying to transform a recipient strain of Aspergillus fumigatus with my gene of interest.Kindly help with an efficient protocol please. I often used 2ug of DNA as my...

16 December 2015 2,960 3 View

What is the best way to clone a 1.2kb insert into a 6.0kb using kpn1 and not1 RE prior to overnight ligation at 4degrees?

I did double digestion overnight,purify and used T4 DNA ligase,incubate overnight again and proceed to transformation the following day having used ratio 1:3 Vector:insert.However few colonies...

04 December 2015 9,161 5 View

Does anyone have an idea on how to get the CPU steal time for Xen Dom-U's on the Dom-0?

Is this possible with Libvirt or there exists some other means? NOTE: This can be done within the Guests themselves but the interest here is to get the stat externally at the host. I have seen an...

12 February 2015 7,516 2 View

In an IaaS (Xen-based) cloud, how much monitoring information can the Infrastructure Provider (IP) get about how each VM on a host consumes resources?

The goal is to be able to manage multiple services on the same host in a Xen environment. Xentop provides CPU utilization for each VM running on the host and we have also been able to do thesame...

20 January 2015 5,362 5 View

What methods are used for finding relationships, relevance and redundancy between multiple continuous variables?

I know about Pearson correlation and Mutual Information, but I am interested in your suggestion of other methods.

09 September 2014 2,687 5 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View

AutoGrid and Autodock 4.2.6 window opens for 1 second then closes and there is no output files?

Good day, I am a student trying to work on Autodock for a project regarding Ligand-DNA interaction so i am quite new to molecular docking. i have followed tutorials and did all the steps...

28 July 2024 2,136 4 View

Christian Q. Scheckhuber

I assume it is very unlikely that a functional protein is synthesized from the modified gene in your mutant strain.

Regards, Christian

Olumuyiwa Igbalajobi

Thanks. I suspect it would be a general problem with the CRISPR cas 9 editing technology if the next ATG could still synthesize the protein. My Prof also submitted same as yours.Thanks once again

You are welcome, Olumuyiwa. Good luck and success with your project.

Cheers, Christian