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Questions related to Sequencing
Values above 7 can be considered ok? I made the RNA extractions using TRIzol. I want to quantify the expression of some immune genes in spleen and head kidney of bacterial infected fish. I also...
18 August 2017 5,514 2 View
Hello everyone, I am trying to construct a plasmid vector with two promoters in opposite direction (one in clockwise and other in anticlockwise direction on circular map). Should I add spacer...
29 July 2017 8,211 3 View
I have to analyze PacBio transcriptome data with four bin sizes: 1-2 Kb, 2-3 Kb, 3-6 Kb and 5-10 Kb. I can understand that the HSP length cut-off and HSP-Hit coverage settings must be customized...
27 July 2017 1,661 1 View
Can anyone recommend software for the design of an sgRNA for use in Staphylococcus aureus with the CRISR system? I have tried other systems, but I would like the system to look for possible off...
26 July 2017 3,241 1 View
splicing reporter minigene assay to evaluate the effect on splicing of unclassified genetic variants
26 July 2017 2,822 2 View
I plan to carry out sequencing of a bacterial integron using random primer walking to identify the structure of internal genes.Is there a program on ncbi homepage that can draw graphics of gene...
24 July 2017 6,512 3 View
Dear all, I have submitted a forward and reverse Sanger sequencing reaction for multiple PCR products. The goal of the sequencing reactions was to analyze single nucleotide polymorphisms to...
23 July 2017 2,911 4 View
We want to sequence RNA from passerine bird blood to look at host expression. We will need to remove the ribosomal RNA and also the globin RNAs during the library preparation. There is no...
17 July 2017 2,110 4 View
Best software for amplicon seq data analysis for malaria parasite. EditI know that there are many software (QIIME,mothur, and others ) to analysis amplicon sequence data for microbial (SPECIALLY...
15 July 2017 4,995 2 View
I need a frequently curated database of Bacterial Insertion sequences which can be downloaded and used as input for standalone local BLAST to ascertain the presence of Insertion sequences in the...
14 July 2017 6,376 1 View
Hi there, I am new to Bioinformatics and I am working on a project that requires me to identify orthologs of ABCC4. I have been using Uniport database, and have been identifying many redundant...
14 July 2017 8,596 2 View
I am trying to figure out how far the promoter/regulatory region of the immunoglobulin variable regions stretches upstream of the startcodon. I am aware that the conserved octamer sequence...
13 July 2017 4,756 2 View
Dear all, I want check the similarity of a list of genes' 5′-UTR regions across different species. Anybody could recommend me to do it in an efficient way, please? Best, Bochao
12 July 2017 5,782 1 View
Hello everyone, Does anyone has primers squances for hpv? and what is the tool to detect their visability? I searched different primers squances using NCBI but non matched the primer sequance I...
10 July 2017 3,599 3 View
There are some >150 types of HPV (human papilloma viruses); I am looking for the conserved regions from their genome.
08 July 2017 1,019 3 View
I have got a sequence from company as I did colony PCR using 16S primers. Now my question, can I make a phylogentic tree using that sequence?? If yes how? Explain. Thanks
08 July 2017 5,554 4 View
Are the black boxes supposed to indicate exons? If so is the furthest black box at the start of the gene the promoter region? This is the - strand so the gene is read right to left.
07 July 2017 6,077 3 View
Hello everyone. Can some one help me?
06 July 2017 5,588 3 View
Sanger Sequencing result shows my insert sequence is perfectly right but, as being said in the question, the vector sequence is a mess. Not only is it about a few random substitution or deletion...
03 July 2017 5,350 2 View
Dear Colleagues and Mentors, Hope you all are doing fine. I'm trying to sequence a gene which has partially sequenced before. Now, our plan is extend the sequence or if possible get the whole...
24 June 2017 8,799 4 View
Has anyone used pig targeted gene expression using MiSeq (Illumina)? I have been looking into the literature but no success.. There are definitely pre-made library kits for Human, Mouse and...
22 June 2017 2,631 1 View
I'm looking for sequencing services between one tube sanger sequencing and Next Generation Sequencing. I need several hundreds reads to analyze a library of DNA, I can't manage it tube by tube,...
21 June 2017 5,341 1 View
I want any information about long chain PCR to detect long deletions.
21 June 2017 4,400 2 View
Hello, I want to look into MiSeq technique for gene expression analysis. I would like to know if there are any tips, guidelines or suggestions. Also if there are other ways to choose a...
20 June 2017 2,494 3 View