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Questions related to Sequencing
BLAST analysis shows 99.7% similarity with a species but morphologically they both have lots of differences !
08 October 2016 6,795 4 View
Sequences of marine invertebrates were run on blast. The attached photo depicts the results. Kindly advice. thank you.
24 August 2016 2,967 2 View
Hi all, As far as I know, TransDecoder, GeneMarkS-T, Prodigal and ESTScan can detect CDSs from assembled sequences. Besides, any others? Thanks.
04 August 2016 9,940 4 View
I was uploading the sequence data to the MG-RAST server, and the notice that sequence stats calculation failed kept popping out when the upload process was about to complete, meanwhile the...
03 August 2016 1,116 4 View
I am trying to amplify an AT rich sequence which is 1543 bp long but still unsuccessful. I have used Phanta Max and Taq enzymes. Please suggest possible solution for it.
07 July 2016 9,063 3 View
I need to identify my fungi, i isolated dna and now which region i will amplify? 18SRNA, 16S RNA or the ITS sequence region?
07 July 2016 2,821 2 View
Hi, I recently upload raw sequence reads to NCBI Sequence Read Archive (SRA). I followed the steps: (1) Make BioProject, (2) Make BioSamples (3) Submit to SRA (Beta) using Aspera Connect. However,...
07 July 2016 2,714 3 View
We used a small amount of our digest (5ul out of 100) and ran on the gel to confirm the digests success, when the rest of the digest sample was quantified with nano drop however, no DNA could be...
07 July 2016 6,327 12 View
For example my 260/280 ratio is 2.08 and my 260/230 ratio is 0.68 measured with nanodrop. Would a bad 260/230 ratio lead to bad sequencing?
07 July 2016 6,051 7 View
Hi, Has anyone used Qiagen's RNeasy 96 Universal tissue kits? How did you find it? Also, what about Macherey Nagel's NucleoSpin™ 96 RNA? Which is better? I have 6000+ bees to homogenate and...
07 July 2016 6,336 2 View
So, I'm about to start this new project and I'm wondering if there are new trending techniques to insert LoxP sites flanking an exon in the mouse genome. We use CrispR/Cas9 system in the lab, so...
28 June 2016 4,789 2 View
I have a 16S rRNA sequence that has 100% of similarity to a bacterial species (Brevibacterium halotolerans ) that is included in a taxonomic group (Bacillus subtilis group). Does that result mean...
22 June 2016 834 8 View
Hi All, My gene is 1248 bp long (that I cloned into a pET-30Xa/LIC vector). I wanted to create a site-directed mutation around 687 bp. I used Agilent's Quik change mutation kit. Reverse sequencing...
06 June 2016 6,460 4 View
I'm planning to repeat MiSeq Illumina run that underclustered. For the beginning, I will increase the load 2-fold to 20 pM. Did anyone tried to add Tris-Cl after denaturation to neutralize NaOH as...
06 June 2016 8,219 7 View
I have sent my sample for dna sequencing, now need to compare my mutated sequence with my data base sequence. so the question os which one is query and which one is subject sequence?
06 June 2016 6,371 3 View
06 June 2016 3,207 8 View
If the Restriction enzyme site is in reverse direction at 5' to 3' end, can enzyme cut that sequence?(RE sequence is in Dark) 5'-GATACCTTAAGGTCG-3' 3'-CTATGGAATTCCAGC-5'
06 June 2016 2,728 5 View
I have amplified the whole mitochondrial genome in 9 fragments and use 62 set of overlapping internal primers for sequencing. I face problems while sequencing. Each fragment is further divided...
06 June 2016 4,884 1 View
I am trying to insert a fragment in pHisGb1, but after sending the miniprep for sequencing, find out the vector is empty. Now how can I improve my ligation?
06 June 2016 443 3 View
Hi there,I have a known fragment of my gene and I need of the 5' and 3' end to assemble them and get a full length of my interest gene. I thing use Inverse PCR, but I have a question. Is it...
06 June 2016 4,454 2 View
Hello contemporaries, any biomedical informatics databases that compares virus genome strains showing the sequence differences via a simple click? (Im interested in comparing adenovrius 40/41...
06 June 2016 7,700 3 View
I have a multiplex PCR with 6 Fwd species specific primers and one universal reverse primer. I usually run single PCRs and after this I clean them with exosap, then cycle sequence each with each...
06 June 2016 9,632 3 View
I've been trying to submit a sequence at Genbank using BankIt. It's an HRP2 exon2 partial CDS of Plasmodium falciparum. Everytime I paste my sequence it says that my sequence contains vector...
06 June 2016 8,150 3 View
Do the sequences in reference databases contain ambiguous N bases? if yes then why is it advised to remove ambiguous bases in quality filtering before mapping to reference??
06 June 2016 6,415 3 View