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Questions related to Sequencing
Hi everyone, I would like to create new raster files: 1) Euclidean distance to coastline 2) compound topographic index 3) elevation and 4) local deviation from global from ETOPO1 data. I have a...
27 June 2022 6,527 1 View
Hello everyone, please l need the gene sequence on OAT, P5CS, P5CR, PDH for mango, l used NCBI database but l could not get sequencing on these genes that l seek. so can anyone share with me other...
02 April 2021 2,692 6 View
Which score should I focus on for that? Thank you!
01 April 2021 4,986 1 View
I am doing metagenomic sequencing without enrichment for an RNA virus. After my RNA is extracted from the blood I am doing library prep with the NEBNext Ultra directional RNA library prep kit for...
17 February 2021 5,501 2 View
I came across klenow fragment when I was reading about HiTS-FLIP method. I know klenow fragment doesn't posses 5'->3" exonuclease activity but what advantage does it provide for ?
02 November 2020 7,917 3 View
Dear All, I have a question regarding the submission of the complete viral genome "FASTA format". I don't know which option should I choose at the features step. It is Avian Corona virus. with...
16 September 2020 7,665 7 View
Hi everybody, I am doing CHIP experiment. The problem is that in my gel agarose I see alot of DNA with big size (longer that 1000 bp) which means there is alot of unfragmented DNA in my gel. I...
07 September 2020 1,779 5 View
Hi everyone, I have a question regarding sequencing SNPs. I have currently creating two SNPs on one gene and these SNPs are about 8kb apart from each other on two different exons. I would like to...
05 August 2020 5,861 3 View
Dear respected community, I wonder if I can get some help on best tool for Taxonomic classification of the bacterial pathogen using the FASTQ file generated from MiSeq? I tried PATRIC but it...
29 July 2020 4,316 0 View
I am interested in mRNAs only (also the ones from chloroplast). RNA input x RNA yielded approximately? Additional Kit used to concentrate RNAs + to get rid of other RNAs (tRNAs, etc...)? Thank...
02 June 2020 1,273 1 View
I'm trying to find homologues sequences of the query sequences by BLASTp vs nr, the results point to sequences belong to two family : "RND family efflux transporter , MFP subunit" with...
11 April 2020 2,934 0 View
We have sequenced Synechococcus sp. PCC 7002 genome. Already the same strain has been sequenced and annotated by many before. Whole-genome map is also available online. The gene of interest is...
18 March 2020 1,271 12 View
Hello everyone! I am thinking to amplify T-DNA insertion region using LBb1.3 (left T-DNA border) and RP (gene specific Reverse Primer) against the genomic DNA of one of the T-DNA insertion mutant...
12 March 2020 1,563 3 View
The question is based on Sanger sequencing results. Gene size is 717bp which falls within the Sanger range. I was curious as to why did one of the reactions of the same sample show odd results...
26 February 2020 8,362 6 View
I am new to HMMs and was thinking to build new HMMs based on the NCBI database(which is available to download) or SILVA, so that it can be used by some other program. Can you suggest any tool...
05 February 2020 6,359 1 View
I am trying to figure out how many sequences I need for my samples. So far I can see that 40,000 would be necessary for microbial soil analysis. I have iDNA samples and so think I will need...
23 January 2020 1,030 1 View
Hi I have 20 RNA samples for miRNA sequencing because of its high cost, Some colleagues suggested pooling the samples, it means, splashing the samples into one or two sample and send for...
07 January 2020 5,321 3 View
Hi I have a Raster file which I want to clip into all the features of a Shapefile. Is it possible to do it all at once or do I have clip each one individually on the Extract by Mask tool...
06 January 2020 8,238 10 View
Dear Researchers, I did Transcriptome of my two Fruit varieties at developmental, ripening and harvesting stage for the comparison of their differential gene expression profile, such as Sugar...
24 December 2019 6,800 4 View
Dear all, I cloned 25bp fragment into a vector. My no-insert ligation has no colonies and my insert+vector ligation has many. Which is the first clue that I have cloned the fragment. Than I...
23 December 2019 2,295 7 View
Dear All, 1 ug of permissible quality FFPE RNA is transcribed to cDNA through SSIV VILO RT kit. I have Qubit v.4 device, which can measure cDNA to a good extent. The cDNA ranges from 25-100 ng /...
12 November 2019 2,285 1 View
I've been doing some RF/Gibson cloning, and I am having issues sequencing my plasmids. I performed colony PCR to identify clones with the appropriate inserts. I then grew these up and plasmid...
22 October 2019 4,860 3 View
What are the criteria for designing biotinylated probes differ from primer designing?
25 September 2019 8,422 2 View
Hi. I amplified about 750bp of DNA from mushroom samples and got smearing bands in QC. what could be the reason? and what happens if I processed these samples for sequencing?
24 July 2019 3,396 6 View