How to fix error link 9999 in gaussian ? Its coming as I add a solvent IEFPCM/DCE Model to the job,without the solvent optimization is sucessful.Why?

I optimized the structure of Pd Acetate Trimer using HF/STO-3G and the B3LYP/LANL2DZ without any solvent. The optimization was successful without any negative frequency. Then I added solvent...

28 September 2020 745 3 View

Does Covid 19 exacerbate anaemia and diabetes in people ?

Can CRISPR-cas9 gene editing technology save patients suffering from low pancreatic beta cell and cure diabetes? If so any human trial going on?

26 June 2020 1,246 4 View

How do i separate a metal complex from DMSO?

Actually ,i couldnot evaporate DMSO due to high boiling point. So how can i get back my metal complex

01 March 2020 3,212 7 View

How to arrange the data for preparing Sample rarefaction (Mao tau) curve using PAST?

I have tree abundance data of six different land use categories and for each category i have taken 12 plots. Now I need to draw Sample rarefaction (Mao tau) curves showing all the six different...

19 September 2019 2,857 2 View

Can anyone suggest me regarding LC MS/MS facility within India for plant based samples?

I want to do LC-MS/MS analysis of my plant based samples (Fractions of plant secondary metabolites obtained from column chromatography) . Can someone please suggest to me some good and reliable...

03 March 2019 9,654 15 View

How to increase the adsorption of graphene oxide on any substrates?

I have deposited thin film of graphene oxide on corning glass and undoped Silicon substrates by dropcast method. I have kept at 50C for 3 hours to dry. Then I have annealed them at high...

03 October 2018 8,794 3 View

Is it possible to get smaller lattice parametrs in thin film form than bulk sample?

I deposited the thin films of MgTiO3 using RF sputtering onto quartz substrate. After XRD analysis of thin films and sputtering target (home made), it is observed that the lattice parameters of...

24 June 2018 4,327 2 View

What theory should be relevant to study the role of New Media in Democracy?

I have been working on a paper on the role of new media (specially social media) in democracy. My emphasis is on the role of social media in Indian Democracy. But could not able to use a...

16 June 2018 1,848 6 View

How to calculate the percentage crystallinity from XRD plot using origin software ?

I have calculated percentage crystallinity using ( Ac/Ac+Am ) formula for the following curves. But I am getting crystallinity percentage more for the sample C in comparison to A. Am I wrong in my...

21 September 2017 5,629 11 View

What are the substitutes to Sodium azide and EMS as cytotoxic agents?

What are the substitutes to Sodium azide and EMS as cytotoxic agents?

01 July 2017 6,909 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View

Repository for capture-seq raw reads?

I am a research tech in a wildlife genetics group and my PI and I are trying to figure out how/where to put raw reads in a repository. We have capture-seq NGS from Rapid Genomics. The post-doc who...

17 February 2021 7,575 2 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

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17 February 2021 4,217 3 View