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Questions related to Sequencing
ChIP sequencing
21 March 2017 460 4 View
After 16S rRNA gene sequencing, I'm trying to design primers to amplify a specific gene before proceed random Mutagenesis. Whole genome sequencing is not possible in my current situation. The...
21 March 2017 4,436 5 View
for example, in case of 16s rRNA nBLAST results, I have almost 80 similar sequences with % similarity ranging from 99% and going down till 94% ?
17 March 2017 4,960 2 View
Dear RG users, I know that a series of genes are expressed in the same tissue of an eukaryotic organism; these genes map in close proximity in the genome, that is they lay hundreds of base...
17 March 2017 4,775 3 View
I need to know the cost of KIR SSO Genotyping Test kit and how many cases we can work if we purchase one kit.
15 March 2017 1,205 2 View
I intend to edit those genes using CRISPR /Cas9 tool
15 March 2017 2,370 3 View
Hi everybody An easy issue that made me confused these days is why ESTs are used for in silico identification of miRNAs or why other types, for instance transcriptome seq data may not be suitable...
12 March 2017 3,737 3 View
I grew up a weird looking fungal colony on SDA at 4C. I isolated it, did a DNA extraction, ran a PCR using fungal primers ITS1F and ITS4. I then sent it for Sanger sequencing. I have the sequence...
10 March 2017 2,915 9 View
The genomes are not annotated, so tblastx or tblastn has to be used to generated the alignment for matching orthologs genes. The only softwareI know is ACT Artemis, which unfortunately does not...
10 March 2017 3,538 2 View
Hello All, I have a cpDNA sequence (Both forward and reverse sequence) of some barcode genes. Can anyone guide me further for gene annotation (Step by step procedure) and submission in NCBI. Is...
03 March 2017 9,910 2 View
I have been given a chromosome number and range (example:chr1:20369369-20490791) and I need to be able to find out if this range falls within another chromosomal range and if it does not how far...
03 March 2017 6,401 2 View
I've been given a large amount (~2500) of ensemble numbers (example:ENSG00000175445) for various genes how can I quickly find the chromosomes they are located on, and the exact section range they...
01 March 2017 6,952 0 View
Hello friends I am analyzing the whole genome sequence of bacterial origin. I would like to know whether there is any user-friendly software to find out the number of genes in internal clusters...
27 February 2017 8,353 3 View
I tried to do the Sanger sequencing using big Dye terminator 3.1 and ABI genetic analyzer 3130xl machine. I have got several results, but it was high peaks at the position around 60-80nt of every...
22 February 2017 9,750 4 View
I am analyzing rDNA sequences from organisms in different countries. One of the locations seems to be very different from the other ones, and when aligning there are blocks of gaps. When I...
16 February 2017 2,685 1 View
Is it possible to do direct sequencing from amplified products of phylogenetic markers?
13 February 2017 728 4 View
Hi all, I have a few questions about next generation sequencing and the generation of the reads. I did not understand some parts of the process..In particular, are there more reads generated for...
08 February 2017 5,782 3 View
Hi all, I know the technology of Microfluidic, Protein Microarrays and Shotgun Mutagenesis. But I don't know how to do it. Your references information is important for me. Help! Help! Help! And...
07 February 2017 1,353 2 View
I have set of genes with their nucleotide sequence (includes exons, introns everything) and I want to extract only coding region from these sequence. 1. How can I do it? 2. Is there any online...
04 February 2017 6,139 3 View
Hello! We have designed primers, which were used for a PCR reaction. We obtained a PCR product size of about 1180 pb (even for the positive control). when sequencing those PCR products, they...
01 February 2017 7,178 4 View
Recently, I came to know about a mobile DNA sequencer launched by Oxford (MinION). 1. What about Its efficiency and the reagents required for sequencing? 2. What are data anlysis tools used, once...
30 January 2017 975 2 View
Is it possible to enrich/selectively isolate poly-dT+ cDNA from a sample of cDNA which was reverse transcribed from total RNA using a mix of oligo-dT and random primers?
30 January 2017 9,299 5 View
The goal is to a align a high number (100-200) of microbial genomes(1-2Mbp) to analyze conserved regions.
29 January 2017 6,937 5 View
Hi every body I want to download all stem-loop and/or all mature miRNA sequences from all eudicotyledons and monocotyledons plants. How can I do that in miRBase 21?
26 January 2017 8,896 3 View