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Questions related to Sequencing
Hello All, I have a cpDNA sequence (Both forward and reverse sequence) of some barcode genes. Can anyone guide me further for gene annotation (Step by step procedure) and submission in NCBI. Is...
03 March 2017 9,875 2 View
I have been given a chromosome number and range (example:chr1:20369369-20490791) and I need to be able to find out if this range falls within another chromosomal range and if it does not how far...
03 March 2017 6,371 2 View
I've been given a large amount (~2500) of ensemble numbers (example:ENSG00000175445) for various genes how can I quickly find the chromosomes they are located on, and the exact section range they...
01 March 2017 6,921 0 View
Hello friends I am analyzing the whole genome sequence of bacterial origin. I would like to know whether there is any user-friendly software to find out the number of genes in internal clusters...
27 February 2017 8,332 3 View
I tried to do the Sanger sequencing using big Dye terminator 3.1 and ABI genetic analyzer 3130xl machine. I have got several results, but it was high peaks at the position around 60-80nt of every...
22 February 2017 9,724 4 View
I am analyzing rDNA sequences from organisms in different countries. One of the locations seems to be very different from the other ones, and when aligning there are blocks of gaps. When I...
16 February 2017 2,655 1 View
Is it possible to do direct sequencing from amplified products of phylogenetic markers?
13 February 2017 695 4 View
Hi all, I have a few questions about next generation sequencing and the generation of the reads. I did not understand some parts of the process..In particular, are there more reads generated for...
08 February 2017 5,749 3 View
Hi all, I know the technology of Microfluidic, Protein Microarrays and Shotgun Mutagenesis. But I don't know how to do it. Your references information is important for me. Help! Help! Help! And...
07 February 2017 1,321 2 View
I have set of genes with their nucleotide sequence (includes exons, introns everything) and I want to extract only coding region from these sequence. 1. How can I do it? 2. Is there any online...
04 February 2017 6,099 3 View
Hello! We have designed primers, which were used for a PCR reaction. We obtained a PCR product size of about 1180 pb (even for the positive control). when sequencing those PCR products, they...
01 February 2017 7,146 4 View
Recently, I came to know about a mobile DNA sequencer launched by Oxford (MinION). 1. What about Its efficiency and the reagents required for sequencing? 2. What are data anlysis tools used, once...
30 January 2017 949 2 View
Is it possible to enrich/selectively isolate poly-dT+ cDNA from a sample of cDNA which was reverse transcribed from total RNA using a mix of oligo-dT and random primers?
30 January 2017 9,269 5 View
The goal is to a align a high number (100-200) of microbial genomes(1-2Mbp) to analyze conserved regions.
29 January 2017 6,917 5 View
Hi every body I want to download all stem-loop and/or all mature miRNA sequences from all eudicotyledons and monocotyledons plants. How can I do that in miRBase 21?
26 January 2017 8,851 3 View
Hi every body, did someone helps me and told me to submit rpoA and pheS sequence genes to Genbank. I did it for 16S sequences, it was easy! But for rpoA and pheS genes I could not find the correct...
26 January 2017 8,262 1 View
I am looking for previous reports that showed sample variations of extracted DNA amount (and quality) from DBS card. There might be huge variations of DNA amount as well as quality of the DNA....
25 January 2017 2,799 1 View
During sequence analysis one of my Chrysaora sp. shows 83% identity with Cyanea sp. Query cover value is 99%. I am sure it is Chrysaora sp. Why this sequence couldn’t show any similarity with any...
24 January 2017 9,717 4 View
SNP’s were got in specific human gene which were sequenced but I want to know these SNP’s already submitted in Indian region, Asian and worldwide of specific targeted gene. So how could I check...
24 January 2017 4,170 0 View
Why are there two patents with same title same inventors and different patent number in one country? For example: Jacobs Jr., William R. US patents:6300061 =>Mycobacterial species-specific...
20 January 2017 1,462 7 View
I have several sequences 30-60 Kbp and I want to recognized a conserved area, I used clustal omega, blast and other software but all of the hanged! I think that because of large size of my...
19 January 2017 1,499 3 View
I found there wasn't termination for antibiotic resistant gene transcription in many vectors. I think stopless transcription will affect activation of following replication origin or another...
18 January 2017 1,966 4 View
Hello, How should I proceed with my raw functional gene sequencing data obtained from Roche 454.Which pipeline should I use to process the sequences?Which database should I use? Any help on this...
17 January 2017 670 3 View
We have successfully amplified and sequenced so far 2 cpDNA regions [rpl32-trnL(UAG) and trnH-psba] and une ribosomal multiple copy of ITS. No success unfortunately with GPA1 that is single-copy....
16 January 2017 3,818 2 View