How to use software Geneious to find genomic features such as genome size, GC content, tRNA genes, CDS etc?
Do a bit of google, you can do this without Geneious - several scripts and tools are floating on internet.
How to calculate Module Young from compressive strength and strain curve
29 June 2024 817 3 View
I prepare Mg doped Hydroxyapatite by wet preipitation but (Ca + Mg)/P ratio is higher than 1,82. CaO peak cannot be detected in XRD spectrum
23 June 2024 8,970 19 View
Here are the ITO material parameters and the Drude model I found in an article, but I don't know how to import these parameters correctly into Ansys lumerical FDTD.The attached picture shows the...
18 June 2024 3,182 10 View
How to create ITO materials in FDTD?
12 June 2024 5,915 2 View
Figure 1 is my plot, the background will be colored when the quantity is set to electron concentration. While Figure 2 is the figure i want. How to set the background to white color when the...
11 June 2024 8,621 1 View
I would like to replace the Zn atom on the surface of ZIF-8 particle by the ion exchange. I have the XRD and TEM data of ZIF-8, how many Zn atoms on the surface of this particle?
18 September 2023 5,138 3 View
Regenerative energy system in EV will be a reserch hotspot in the future?
20 August 2023 8,125 1 View
Hello everyone, I am currently doing transient transfection. I want to transfect the overexpressed plasmid into HCC cells, and this plasmid has 10000+ base pairs. The transfection reagent I used...
15 July 2023 3,881 3 View
When I do the PPC-PC reverse dry transfer method, PPC and the sample fall on the PC, and then I melt the PC/ sample /PC onto the silicon chip, how do I remove both PPC and PC to get the sample?
01 July 2023 4,026 1 View
Leading skills include the ability to inspire and motivate teams, fostering a collaborative and high-performing work environment. Employees with leading skills can effectively communicate...
30 June 2023 4,696 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...
28 July 2024 6,938 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View