How to use software Geneious to find genomic features such as genome size, GC content, tRNA genes, CDS etc?
Do a bit of google, you can do this without Geneious - several scripts and tools are floating on internet.
Dear All, I would like to know if total phosphate (TP) and total nitrogen (TN) in soil can be determined with UV-Vis spectrophotometer or not. If somebody knows the method, I would like to get...
03 January 2021 7,662 6 View
Can anyone tell me how to simulate noise of CMOS image sensors, such as thermal noise and flicker noise with sentaurus TCAD?
09 November 2020 9,616 4 View
Hi all, I am using 96-well plates to culture iPSC derived neurons, the problem is, when doing ICC, my neurons tend to detach during the washing procedure, till the final step nearly all neurons...
14 July 2020 3,828 7 View
I have three mice but I only need to scarify two in my experiment. Can I keep the leftover one mouse in the cage with itself? The other option is to put it in another cage with other mice. But...
12 July 2020 307 5 View
Ammonium chloride is a lysosome inhibitor and a weak base as described in a published paper, Ammonium chloride, an inhibitor of phagosome-lysosome fusion... . However NH4+ is a weak acid, I...
21 April 2020 8,419 3 View
Does some have got the commentary of the American norm ANSI MH 16.1: 2012? according to this norm the seismic design of the steel rack could be performed using R=4 as behavior factor, this value...
19 November 2019 6,598 2 View
I need to clone a 5415 bp gene into pglo plasmid replacing it's GFP gene which is around 700bp long. Now PCR amplification of this 5415 bp gene using PhusionR HiFi Taq doesn't show any band, only...
05 October 2019 4,938 7 View
I stained the cells with DAPI after treated with chemicals and then checked with fluorescence microscrope. I would like to know what is the morphology of these cells.
09 August 2019 9,328 5 View
I am working with phospho protein in western blot and have problems about the phospho protein signal. It is common that researchers can not detect phospho-protein signal in the membrane, as...
17 July 2019 6,027 3 View
I was trying to imaging the 2D materials with aberration corrected TEM, including MoS2, ReS2 and graphene. However, I found serious carbon contamination when zoom in at the region of interest....
30 May 2019 9,828 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?
28 February 2021 8,095 1 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...
24 February 2021 4,397 3 View
I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...
23 February 2021 8,969 1 View
I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...
23 February 2021 9,007 3 View
I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...
22 February 2021 4,374 3 View