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Questions related to Sequencing
I have homologous sequences with more than 80% identity. I wonder these genes might have undergone gene duplication which resulted in their different activity (function), i.e. they are paralogs. I...
19 December 2016 4,120 11 View
Would there be any potential issues that anyone could see arising by using the RACE ready cDNA for my regular two gene specific primer PCR.
19 December 2016 8,291 1 View
I have a data set from a metatranscriptmic sample and I want to recover the 28s ribosomal sequence of one organisms present in sample. The sample had a a ribosome-depletion step before sequencing,...
18 December 2016 1,071 3 View
anybody know the reference or housekeeping gene of coconut hispine beetle brontispa longissima for qPCR? thanks
16 December 2016 3,521 1 View
I was following the tutorial/methods. I realized that the pipeline was applied on single-end sequencing data. I wonder if anyone has done it on paired-end data especially on the step of converted...
15 December 2016 3,742 1 View
I want to search any possible synteny of genes in both coffee and tomato genomes. I have genes sequenced and looking for if these genes maintained the same organization in tomato genome as they...
14 December 2016 774 1 View
I'm trying to figure out the best sequencing service to use in terms of price, service, quality of results, ease of sample collection, etc. What should I be considering when I select a sequencing...
13 December 2016 2,812 3 View
I am looking for a reference about the error rate in pacbio sequencing machines and how it has evolved in the recent years. I'm also interested in learning about it's randomness and how errors can...
12 December 2016 3,199 2 View
Hi everyone, I started a MiSeq run today and everything checked off as fine and I started the run. It finished in less than an hour and said "sequencing complete." However, there was not one...
12 December 2016 6,068 3 View
We are tying to sequence and build de novo transcriptomes from total RNA for phylogenomics of non-model organisms (Lophotrochozoa), the degraded samples cannot be re-collected.
08 December 2016 7,126 2 View
Am working on a project that needed me to generate one million records in a single table and i tried it on Oracle SQL Developer Database it truncated the inputted data, I also tried with XAMP...
08 December 2016 2,284 2 View
I am sequencing extracellular vesicle RNA on illumina Nextgen 550, on paper for this kit looks great... but has anybody experience with it?
04 December 2016 2,631 0 View
I need compare a particular protein coding (nucleotide) sequence and amino acid sequence with vertebrates and non-vertebrates and understand the mechanisms of evolution and relatedness using...
04 December 2016 3,538 3 View
I have a MR volumetric sequence, where size of the brain volume is 256*256*181. Where there are 181 sequences, each sequence size of 256*256. I want to extract patches from each sequence and...
02 December 2016 9,589 3 View
I am working on some protein and would like to know about probable candidate protein interacting with my query. sequence and protein structure is available with me. Please suggest me some good...
30 November 2016 1,338 2 View
I'd like to capture genomic fragments around 200-300bp length. Has someone tried this? Which bait length would you recommend?
29 November 2016 1,228 1 View
Hi collegues, I am trying to get a great tool to despict the phylogeny data but TCS nor Network are useful when ambiguity IUPAC codes are present in the sequences. If someone knows how resolve...
25 November 2016 2,315 4 View
I want to amplify a 1500bp amplicon, when I use pfu DNA polymerase the PCR product size is 1000bp, when I use taq DNA polymerase it is 1500 bp. Do somebody have some comments in this regard?
24 November 2016 2,267 5 View
I am trying to find out the latest information on how many bacterial genomes have been sequenced and how many fungal. I have looked on NCBI but can't find any actual numbers. Does anyone know...
24 November 2016 3,229 3 View
I need a lab that is renowned and capable of conducting gene expression and sequencing..... Thank you.
23 November 2016 5,586 4 View
I am using HPLC-UV/FLD Agilent and get this error Failed to schedule the sequence "object reference not set to an instance of an object" in HPLC-UV/FLD. Would you have any suggestions to fix this...
23 November 2016 7,570 3 View
I have a fasta sequence, which i want to represent as a Line DNA barcode. Kindly suggest any online tools, softwares etc. Thank You
22 November 2016 1,804 4 View
Is anybody familiar with uploading Sentinel 3 data in ArcGIS or QGIS program? I have problem and to load in to a SNAP!
22 November 2016 3,641 6 View
could anyone provide such a successful MTS sequence that works in arabidopsis?
17 November 2016 8,311 1 View