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Questions related to Sequencing
Hi, just want to know your experiences. Gene synthesis companies and also addgene start to use NGS for sequence validation. thus it seems that prices may have dropped to a degree where it becomes...
20 April 2017 2,342 6 View
Is there a way to annotate features in Serial Cloner that share sequence, such as a primer and a fragment of inserted genomic DNA carrying a CDS? This is possible in MapDraw (Lasergene), but...
20 April 2017 6,135 1 View
Hi all, Is there any new and reliable mini sequencer that is recommended?
17 April 2017 2,928 3 View
we have performed assembly using both CLC work bench (average length: 674 bp; N50: 890 bp) and TRINITY (average length: 725 bp; N50: 928 bp) and performed the analysis from both of them...
17 April 2017 6,938 4 View
I'm seeing a second call inside most peaks of my DNA sequencing data. This second (smaller) peak is exactly the next base called. Has any one seen this before? Is this a problem with primers? I'm...
17 April 2017 7,426 11 View
Hi there, I have recently assembled a NGS HiSeq data with ABySS 2.02. With the default setting (K-mers=64) I had a 183nt stretch of N (or Gap). I have changed k-mer to 40 and the gap reduced to...
15 April 2017 1,033 3 View
can anyone plese let me know how to clean phage contaminated plasmid.
12 April 2017 3,989 4 View
....? ....?
09 April 2017 8,835 1 View
Dear All, I have little doubt. Normally for the species level identification we all will under go for the DNA Sequencing using designed Primer....is it possible to identify the species by...
06 April 2017 1,796 5 View
I want to amplify mer genes from bacteria. Can you suggest any primer or software for primer designing?
06 April 2017 207 7 View
In Sanger sequencing, what are the possible reasons a particular point mutation to become homozygous from the Forward primer while being the same mutational point a heterozygous in the...
04 April 2017 3,321 3 View
There are different primers used in sequencing of D Loop region of mitochondrial DNA in horse. Will different primers not result in different sequences? and Can the sequences obtained using...
30 March 2017 6,256 6 View
I'll be using pET-44a and I can't seem to find a download of just the vector sequence (preferably with annotations :D). I'm working with CLC and Serial Cloner. I've tried...
28 March 2017 5,222 2 View
I am new to Chip-seq. Currently I am working on a bacterial transcriptional regulator. We do have a few putative binding target genes for the regulator. So I wanted to use qPCR to evalutate the...
27 March 2017 1,630 3 View
I mean sequencing + assembling. The genome of that species has been already sequenced.
24 March 2017 5,350 3 View
ChIP sequencing
21 March 2017 433 4 View
After 16S rRNA gene sequencing, I'm trying to design primers to amplify a specific gene before proceed random Mutagenesis. Whole genome sequencing is not possible in my current situation. The...
21 March 2017 4,418 5 View
for example, in case of 16s rRNA nBLAST results, I have almost 80 similar sequences with % similarity ranging from 99% and going down till 94% ?
17 March 2017 4,941 2 View
Dear RG users, I know that a series of genes are expressed in the same tissue of an eukaryotic organism; these genes map in close proximity in the genome, that is they lay hundreds of base...
17 March 2017 4,742 3 View
I need to know the cost of KIR SSO Genotyping Test kit and how many cases we can work if we purchase one kit.
15 March 2017 1,182 2 View
I intend to edit those genes using CRISPR /Cas9 tool
15 March 2017 2,334 3 View
Hi everybody An easy issue that made me confused these days is why ESTs are used for in silico identification of miRNAs or why other types, for instance transcriptome seq data may not be suitable...
12 March 2017 3,705 3 View
I grew up a weird looking fungal colony on SDA at 4C. I isolated it, did a DNA extraction, ran a PCR using fungal primers ITS1F and ITS4. I then sent it for Sanger sequencing. I have the sequence...
10 March 2017 2,896 9 View
The genomes are not annotated, so tblastx or tblastn has to be used to generated the alignment for matching orthologs genes. The only softwareI know is ACT Artemis, which unfortunately does not...
10 March 2017 3,516 2 View