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Questions related to Sequencing
please, I need to design probe as negative control in my experiments and i need to use Lac Z but i dont know its sequence can anybody help me in this. I will use it in mouse and human cell...
05 May 2016 4,202 1 View
Hello, everyone. Recently, I did chip-seq (H3K4me1、H3K4me3、H3K27ac、H3K27me3、Pol II、P300) from 1×107 insect cells. But, I got poor yield (about 4ng ) as standard Illumina library preparation needs...
05 May 2016 6,683 6 View
I want to start with some CRISPR experiments (with the nickase system) and I am wondering what sequences I should use for a non-target control? I am probably overthinking this, but at the moment I...
05 May 2016 5,296 2 View
Use of CRISPR/Cas9 on SORBIDRAFT_01g047430 and design of gRNAs.
05 May 2016 8,432 3 View
i selected a sequence for modelling currently it is not having experimental structures but model available in protein model portal.Can i model it and publish?
05 May 2016 7,820 2 View
We could observe similar stop codons in matching NCBI sequences. Is it justified
05 May 2016 837 3 View
I am working on setting up a CRISPR/Cas9 experiment. I have successfully cloned my sgRNAs, but due to the size of my deletion, will need to use a vector template instead of an ultramer. The...
05 May 2016 8,265 3 View
Someone here have been using the Duke sequencing facility ( (https://dugsim.net/estimate_cost). What about the reads/cluster quality? Thanks.
05 May 2016 7,049 1 View
Hi all, Are you familiar with Bioedit in the visualization of the sequences? I am trying to read sequences coming from Sanger sequencing method and I was said that the "genuine" sequence is...
05 May 2016 2,502 3 View
I want to sequence some unknown protein excised from 1D-SDS-gel. Have anybody experienced with protein sequencing? Are there any good commercial protein sequencing services?
05 May 2016 4,518 1 View
Does anyone know which isoforms in flybase that dPAR-1 N1S and NIL correspond to?
05 May 2016 4,134 1 View
I have different transcriptome libraries for RNA seq analysis.I am using CLC genomics workbench for the same analysis.The problem is that I am getting more than one adapter sequence during adapter...
05 May 2016 8,796 9 View
I have a file with hundreds of rsIDs in pairs. I want to test if each pair is in linkage disequilibrium. Is there a tool that can calculate all of them at the same time? My file looks like this:...
05 May 2016 6,686 3 View
Hello all, After googling for some hours I realized many people have had this problem but I couldn't find a satisfying explanation. I'm mutating some cysteine residues to serines using the...
05 May 2016 9,233 3 View
Molecular identification Suggest any procedure best for toxic algae identification using 16srRNA
05 May 2016 3,247 5 View
I mean: how many bases correspond to each exon? I came across a site were you could see how each exon fits with the previous and following one, but I don't remember which page was it :( . It would...
04 April 2016 1,589 2 View
based on poisson distribution, probability of each nucleotide will be sequenced is P(Y=y)= (Cy)(e-c)/y! y=number of times base is sequenced C= coverage =NL/G = (#reads)(length of read)/genome...
04 April 2016 5,575 2 View
Since I am working on Cryptosporidium, the sequences that I am studying show length polymorphisms because of which all sequnces are not of equal length. Please tell me how do I analyze such...
04 April 2016 7,190 3 View
Additionally, I would like to know how the emulsion melts and how the beads are placed on a flat and solid surface? Thank in you very much in advance! Best regards,
04 April 2016 3,551 1 View
Copper deposit in the study area has formed in the geenschist and it showed supergen to hypogene sequences, then we need related reactions about these Cu minerals.
04 April 2016 3,612 3 View
I'm using the QuickChange II SDM kit with complementary primers to make expression vectors carrying a mutation My vector is about 9 kBp and I follow the standard SDM protocol from the provider of...
04 April 2016 5,970 4 View
this vector contains the pL promoter
04 April 2016 2,877 1 View
I want to sequence a putative gene
04 April 2016 8,805 3 View
I f i used Microarray to study transcripteome of bacteria under two different conditions i need to confirm with RT-PCR. Does Next generation sequencing also need confirmation?
04 April 2016 9,247 2 View