Mouse antibody sequence anomaly?

18 May 2017 1 9K Report

Hi, I have been trying to sequence a mouse antibody but I get a +1 frameshift at the CDR3 junction for both light and heavy chains. Does anybody know what is the right sequence and why it does this? I have cloned it several times using different vectors and primers but it keeps giving these results. For example when I reach the cysteine prior to the CDR3 I get a couple of amino acids then a stop codon but if I continue on the +1 reading frame I get the correct end of the sequence. Any ideas please?

Paul Rutland

can we see the sequencing .abi electropherogram file please?

thank you

Badges
Science method

More Francois Bertelli's questions See All

Is Analysis of Similarity (ANOSIM) sensitive to differences in sample size?

Hi there. I want to compare 10 small forest landscapes on which different harvest designs were applied and see how much their attributes (structure, species, soil moisture...) differ. ANOSIM seems...

29 March 2020 5,826 4 View

Is there a way to download all molecules from a published research paper?

Hello, Is there a (preferably free) way to download all molecules (SMILES or SDF) from a published scientific article? Like searching pubchem or chembl or ZINC via DOI or so? I would like to...

05 January 2020 7,542 3 View

How to add a journal ?

Hello, is it possible to add a journal ? (Les Cahiers de Neuropsychologie Clinique) Some of my references can't be complete. Thank you.

08 October 2019 8,622 3 View

What would be an appropriate method to compute the watershed of a lake?

Hi there, I'm wondering what would be the best approach to create the watershed of a lake. I see two solutions here: - Creating the watersheds of all tributaries of the lake and aggregate them,...

02 March 2019 2,527 3 View

Is surface water supply a hydrologic service, a forest service, or a watershed service?

There is no doubt that the access to surface freshwater is an ecosystem service. However, I feel that the many publications on ecosystem services and disservices with respect to the water supply...

07 January 2019 4,166 13 View

What are your tips for obtaining maximum resolution on an agarose gel?

I want to resolve very small deletions on agarose gel. My fragments are ~ 200 bp. What are your tips to get the best possible resolution? Here is what I have already gathered around: - 4%...

18 November 2018 5,740 6 View

What is your favourite long-range PCR enzyme?

I want to amplify a long region of around 20 kb from human genomic DNA. I understand this is around the maximum of what is feasible, but looks possible, at least according to manufacturers'...

17 June 2018 4,218 6 View

I am looking for post-doctoral position that may begin in the fall. Can you help?

I defended my dissertation for the Payson Program for Global Development at Tulane University in May and I am currently working on the required edits. The title of my dissertation is "A...

05 June 2018 3,032 0 View

How to install Neo (Python package for working with electrophysiology) for MAC?

I am wondering if it is possible to install Neo (Python package for working with electrophysiology) http://neo.readthedocs.io/en/0.5.0/ on a Mac. If yes, how to do it?

21 February 2018 2,857 1 View

Anyone using Brookhaven BIC90 for zeta potential measurements. What is the polymer of the Organic Solvent Electrode?

I need to measure zeta potential of dispersions in organic solvent NMP DMF. I can't seem to find the material of the electrode, what polymer and it is compatible with the solvents.

02 January 2018 8,876 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View