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Questions related to Sequencing
Hi every body, did someone helps me and told me to submit rpoA and pheS sequence genes to Genbank. I did it for 16S sequences, it was easy! But for rpoA and pheS genes I could not find the correct...
26 January 2017 8,310 1 View
I am looking for previous reports that showed sample variations of extracted DNA amount (and quality) from DBS card. There might be huge variations of DNA amount as well as quality of the DNA....
25 January 2017 2,829 1 View
During sequence analysis one of my Chrysaora sp. shows 83% identity with Cyanea sp. Query cover value is 99%. I am sure it is Chrysaora sp. Why this sequence couldn’t show any similarity with any...
24 January 2017 9,764 4 View
SNP’s were got in specific human gene which were sequenced but I want to know these SNP’s already submitted in Indian region, Asian and worldwide of specific targeted gene. So how could I check...
24 January 2017 4,234 0 View
Why are there two patents with same title same inventors and different patent number in one country? For example: Jacobs Jr., William R. US patents:6300061 =>Mycobacterial species-specific...
20 January 2017 1,531 7 View
I have several sequences 30-60 Kbp and I want to recognized a conserved area, I used clustal omega, blast and other software but all of the hanged! I think that because of large size of my...
19 January 2017 1,549 3 View
I found there wasn't termination for antibiotic resistant gene transcription in many vectors. I think stopless transcription will affect activation of following replication origin or another...
18 January 2017 2,022 4 View
Hello, How should I proceed with my raw functional gene sequencing data obtained from Roche 454.Which pipeline should I use to process the sequences?Which database should I use? Any help on this...
17 January 2017 741 3 View
We have successfully amplified and sequenced so far 2 cpDNA regions [rpl32-trnL(UAG) and trnH-psba] and une ribosomal multiple copy of ITS. No success unfortunately with GPA1 that is single-copy....
16 January 2017 3,877 2 View
Currently conducting cDNA amplification during a Tag-Seq (RNA-Seq) protocol. In the gel attached, all wells with 'D' should be cDNA with smears, all others should not because they are controls...
14 January 2017 3,809 1 View
I want to have sequences of a list of genes of several bacterial species. Many of these genes are not available on NCBI and other sources. But the whole genomes of the bacteria are quite a lot...
12 January 2017 6,102 6 View
Hi Everyone, i have a Sequence of 16s rRNA, when i tried to generate CAP- Contig using BioEdit, am getting Multiple Contigs for a particular strain, but when i tried with some other strain am...
11 January 2017 719 2 View
I have searched the literature and NCBI to no avail. I'd appreciate your help!
11 January 2017 9,179 2 View
I extracted DNA from blood and made bisulfite conversion. I did PCR (without cloning) and then sanger sequencing. I have an amplicon of 255 pb in a UTR gene. I also did PCR for the untreated DNA...
10 January 2017 5,482 4 View
Hi , I am new with the Illumina sequencing and I got back the sequencing data. I am quite new with the terminology. Can you explain to me what it means that a project will be sequenced using...
10 January 2017 7,757 12 View
I use vent exo- polymerase for amplification the template below but it seem not get high yield. Could you give me some advice? 5’-A (CTG)10 A AAG TTA GAA CCT ATG-3’
10 January 2017 2,369 5 View
Hi, Im learning about next gen. seq. So, with NGS you shear up dna into fragments and when the computer tries to align by matching overlapping regions isnt there a possibility of the fragments...
09 January 2017 345 4 View
Hi all. I need to evaluate Polyphenol oxidase (PPO) protein content in olive fruit extracts by Western blot. So I'm searching also a housekeeping protein in Olea europea for normalization. Some...
09 January 2017 5,449 3 View
How do I edit a gene manually after sequencing?
08 January 2017 1,192 3 View
I want to find out conserved domains of diacyglycerol acyltransferase in several algal species. But I am having difficulty to get the dgat sequences of C renhardtii. I will appreciate any help..
08 January 2017 5,497 2 View
I have approx 3000base ssDNA (3'-5', synthesized by asymmetric PCR) and complementary RNA (5'-3' ; synthesized by T7-transcription). I want to prepare DNA-RNA helix (hybrid) in solution. I have...
05 January 2017 3,546 1 View
We are trying to FACS sort shh-responsive cells from injured mouse facial nerve and run RNA-seq on them. I have limited bench experience. Any advice or detailed protocols for these procedures? I...
28 December 2016 1,758 2 View
I'm relatively new to Reverse transcription. I have worked with cDNA before but dealing with RNA extraction and reverse transcription is new for me so bare that in mind. I'm studying the...
27 December 2016 851 2 View
I am using 16S rRNA sequence for bacterial phylogeny construction.
24 December 2016 6,909 8 View