Satellite orbit estimation using Recurrent Neural Network - what should I use?

Hello, I am a master's student studying in Yonsei Univeristy, Korea. I am trying to estimate the state of satellite, using Neural Network. Below is a simple flow of my study. 1. Train (t0 ~ t1)...

23 February 2021 6,383 3 View

How could be improved the farmers' farm income in reality point of view as well as double the farm income?

At present central government making the policy and forecasting for doubling the farmers' income, and on the other hand here working a no of research institutions in India and abroad for long time...

09 November 2020 1,025 12 View

MAb detecting two viral proteins?

Hi, What are the chances that the developed MAb can detect two viral proteins simultaneously? The MAbs were raised against purified viral particles. During the characterization studies, some...

28 May 2020 4,061 1 View

How should we start our Dental Practice after lockdown because of COVID 19 ?

Dentistry being the one of the high risk profession for the COVID 19 outbreak transmission. For now we are in the lockdown period and only providing the Emergency dental treatment services with...

23 April 2020 5,155 35 View

Can anyone suggest me the best suited shape for quantum well based LED device?

Among different shapes of well, which is the most suited well in terms of efficiency

18 September 2019 7,798 1 View

How reasonable is it to apply Trouton's rule to melting?

Trouton's rule says that entropy of vaporisation is almost same (85 J/ (K.mol)) for lot of liquids near melting point. This usually works out as the entropy of gas is much higher than that of...

10 March 2019 1,669 10 View

What is the maximum holding time for metal analysis after acidification to less than 2 pH?

Maximum holding time for metal analysis

18 September 2018 9,260 1 View

Why an unexpected single band in SDS-PAGE of purified IgG from ascitic fluid using AbFRONTIER Protein G Sepharose Fast Flow column?

I have tried to purify the IgG from mouse ascitic fluid using Abfrontier Protein G Sepharose fast flow column according to manufacture's protocol. The nanodrop reading is showing concentration of...

14 May 2018 3,673 7 View

Is it possible to code a finite element package, I.e. Can an engineer download an application, insert their dimensions & the FEM will build the model?

I want to code an application which will allow an engineer to insert there dimension, material properties and a finite element package will build the model, the engineer will just have to run the...

22 January 2018 6,536 7 View

What is the difference between a primer used in the SYBR green based real time PCR and a TaqMan probe, if both are targeting a single gene?

Whether the same primer sequence (Used in the SYBRG-based qPCR) can be used to prepare TaqMan probe, if they are specifically amplifies the target? If not, what would be the exact difference or...

11 December 2017 360 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View

Repository for capture-seq raw reads?

I am a research tech in a wildlife genetics group and my PI and I are trying to figure out how/where to put raw reads in a repository. We have capture-seq NGS from Rapid Genomics. The post-doc who...

17 February 2021 7,575 2 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

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17 February 2021 4,217 3 View