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Questions related to RNA Extraction
I try already 2 protocols but there are not good enough, the nanodropo and the electrophoresis gel does not give us good results!
18 July 2017 8,627 3 View
I plan on isolating total RNA from exosomes of serum and plasma and would want to use a column based kit, since Qiagen miRNEasy kit is not fetching great results. Can I use RNeasy mini kit to...
18 July 2017 4,017 3 View
Hi, i would like to know if anyone tried the Direct-zol RNA extraction kit by Zymo Research. They claim it to be superfast, have a good yield and be able to purify also small RNAs. I'd use it on...
17 July 2017 9,957 2 View
Sampling methods for plant RNA study were different from the previous studies related the gene expression. Some researcher was collected the youngest leaf. Another researcher was collected fully...
13 July 2017 4,886 5 View
We used FNA paraffin-embedded hepatic tissues from our study population to evaluate some gene expression. But already we got week results in RNA extraction and also in RT-PCR. We extracted RNA by...
12 July 2017 2,055 3 View
We had the following A human blood sample had RNA concentration of 142,85 ng/ul, we used 3,43 ul in 20 ul (with a mass of 490 ng) using the iscript kit. Nanodrop reading for this blood sample...
12 July 2017 9,251 3 View
Has somebody know a protocol to simultaneusly perform Edu and in situ fluorescent hybridization to detect mRNA expression?
09 July 2017 7,310 0 View
I am doing an rna extraction from tomato leafs, using the Trizol protocol. But when I add chloroform, and centrifuged the samples, the hidrofilic portion gets a pink coloring. And the color keeps...
04 July 2017 6,737 6 View
Hi, I am working with cultured cells and I need to send some samples abroad for RNA extraction. I do not have Trizol reagent or RNAlater as yet, I ordered but I won't get them in time to ship my...
03 July 2017 5,224 4 View
I'm currently working with an organism which has no established endogenous control mirna that I could use to normalize qpcr data. I'm thinking about instead using an exogeous control mirna such as...
28 June 2017 1,696 3 View
My aim is to measure microRNA using Luminex from archived samples, so basically formalin fixed paraffin embedded, and I don't know if I can extract good quality (and stable) RNA from this kind of...
26 June 2017 4,253 2 View
I'm having problems with qpcr. This primer have been tested in another lab and it worked fine. It has late amplification (like 30 cycles) but in my samples I get amplification in like 45 cycles....
21 June 2017 2,341 2 View
The PBMCs were suspended in RNALter and then stored at -80oc. Now I want to retrieve the PBMCs and then extract the RNA (inc the microRNAs) - does anyone have advice about centrifuge speeds/...
20 June 2017 6,168 3 View
Hi all, So I want to freeze zebrafish embryos to later (1 week) extract RNA with trizol + RNeasy kit an perform RNA-seq. Would you freeze the embryos in TRIzol or in RNAlater? Does to freezing...
19 June 2017 4,256 4 View
Has anyone had success with isolating RNA from FFPE sections that was sufficient in quantity and quality to make cDNA for RT-PCR? If so, what kit/protocol did you use for the RNA isolation, how...
16 June 2017 7,874 1 View
Hi all! :) I want to extract RNA from zebrafish embryos, 3dpf. My limitation will be the number of fish needed to gain enough material after (1) extracting total RNA (2) doing poly A...
15 June 2017 5,435 7 View
Hi, I would like to extract RNA from biofluids, and we have a home-made protocol that I got from a collaborator, that uses Zymo spin columns. However, we have bunches of miRNeasy micro minelute...
14 June 2017 8,483 1 View
If yes then how long can you keep such tissues in -80C without the getting degraded?
13 June 2017 947 1 View
Badly need some help as I did not find any exact protocol for my experiment anywhere, I am working with human platelet to find out the effect of charcoal on the expression of platelet glycoprotein...
12 June 2017 6,241 3 View
hello do Whatman® FTA® cards preserve RNA of a sample? for example, I am using Whatman® FTA® cards to collect Blood and saliva samples and kept them for 1 month at room temperature.. is RNA preserved?
04 June 2017 7,410 1 View
Since heparin inhibits reverse transcriptase, heparinase I treatments prior to qRT-PCRs are recommended. NGS also uses reverse transcriptase, so I was wondering whether anyone has tried doing a...
31 May 2017 490 1 View
We usually use this kit which has a qia shredder column to lyse the cell before transferring the lysate onto another column for further RNA purification. However we have run out of this qia...
23 May 2017 969 3 View
Hi! I am need to isolate mRNA from cells for MS analysis. I used Trizol/Chloroform RNA extraction and then one kit for poly(A) selection for the mRNA isolation but I am looking for other methods...
22 May 2017 3,191 1 View
I am dissecting the Hippocampus in cold PBS and homogenizing directly in TRIZOL, but despite that I am getting RQN close to 4.
18 May 2017 5,125 6 View