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Questions related to RNA Extraction
Hi everyone, I am isolating RNA from pine cambium and am having problems getting clean pure RNA. If I use a miRvana kit I get around 10ng/ul RNA and 0,5 ng/ul DNA and by LiCl precipitation I get...
30 July 2018 638 2 View
I am trying detect a rna virus in stool samples. To extract the rna, I have been using qiagen viral rna extraction kit. From cultured viruses I had good results but all stool samples came out to...
28 July 2018 2,947 3 View
I have tried so many times to extract tRNA from Rose leaves and buds by pBiozol method. My intention is to have tRNA for experiments about miRNAs (as pBiozol can give tRNA with high concentration...
21 July 2018 6,415 18 View
Hi, I am having issues loading both the classifier model and rules from Cell profiler analyst into cell profiler in the filter object module. I created a pipeline where I identify my primary...
18 July 2018 8,514 3 View
Hi, I usually use sucrose lysis buffer ( 0.6 M sucrose, 0.02 M Tris base, 0.01 M MgCl2 and 2% triton) for RBCs lysis in DNA extraction. After initial RBCs lysis and washing, a pellet of WBCs is...
11 July 2018 5,636 2 View
I am trying different methods for my enrichment culture which contain lots of Fe(II) and Fe(III) minerals (oxidized from 10mM FeCl2). The amount of total cell numbers are around 104-105 cells/ml,...
15 June 2018 6,709 4 View
Hello everybody. Does anyone know if I could freeze my infected THP-1 macrophages before extracting RNA? The reason to do so would be that I would like to perform controls to check if the cell...
30 May 2018 9,938 2 View
I am planning to use the Mobio Power Water RNA Extraction Kit (50) and am inquiring about the probable effects with a sample that has been through the RNEasy Protect Kit. The RNEasy protect kit is...
21 May 2018 9,251 3 View
I have some samples (few cells of ciliates) are preserved in RNAlater. So is there any problem if I start my work by adding lysis buffer for RNA extraction in my preserved samples stored in...
17 May 2018 8,726 3 View
Hi! I need to extract RNA from sorted immune cells using RNeasy Quiagen minikit , and having many samples I would process them approximately 2 hours after being sorted. Because of this delay, I...
11 May 2018 8,297 5 View
Hello, I need help in constructing my experiments in detecting the presence of a bacteria in my plants, via dna extraction I have 10 treatments, with 6 replicates and 5 plants unit per replicate,...
16 April 2018 5,634 3 View
I want to know the artificial gene or strain which can be use as a DNA/RNA extraction control, and will lead you to RT PCR testing for any viral DNA or RNA.
11 April 2018 8,607 4 View
I'm making nucleic acid extraction with Maxwell RSC equipment. But the purity 260/280 is not too high. I extract the nucleic acids from plasma samples. So I'd like to know if there's anyway to...
13 March 2018 5,208 4 View
Hello, For the project I am currently involved in, I am in need for a cheap, non-toxic, pH 7+, non-disruptive buffer for RNA stabilization of bacterial cells (applied to a large environmental...
05 March 2018 5,402 14 View
Does anyone use the 'SPLIT RNA Extraction Kit (lexogen)'" for heart tissue? It would be nice if you could share your experience. I means the data and some tips. Thanks! The linkage of the...
02 March 2018 2,228 3 View
Hello all, I want to collect Arabidopsis leaves samples exposed to different stress conditions, at different time points. I want to know instead of going for liq. nitrogen can i store my samples...
26 February 2018 10,012 2 View
Hi everybody, Im trying to isolate RNA from umbilical cord total tissue by nitrogen freezing and powdering and then Qiazol/Choloroform extraction. I have obtained good results with placental...
14 February 2018 3,942 4 View
I'm interested in isolating neutrophils from human whole blood for subsequent RNA extraction for downstream purposes like qPCR. Just wondering if anyone is willing to share their protocol/work...
02 February 2018 9,500 2 View
I would like to avoid grinding the tissue with liquid nitrogen and a pestle and mortar. Are there other ways to start RNA extraction from plant tissue stored in RNA later? Can it be freeze-dried...
01 February 2018 758 3 View
Dear all, attached are the images of a hydroid species collected from the east coast of India. Can anyone identify the species please? Only fragments of the animal was collected from a bottom...
24 January 2018 1,528 0 View
Hi dear colleagues, I work on altered expreeion profiles genes in bread wheat against mycotoxin of fusarium head blight.I need information about Sampling and RNA extraction from wheat spike. I'm...
07 January 2018 2,936 3 View
I am trying to extract RNA from the pseudo bulb part of a medicinal terrestrial orchid. although I got high quality RNA from leaf but while trying so from the pseudoibulb or rhizome I am facing...
28 December 2017 2,914 1 View
HIV RNA Extraction
15 December 2017 6,681 2 View
It is said that root sampling is a critical task to be done since it would influence the quality of your samples. I would like to ask for a root sampling procedure in rice which takes in to...
28 November 2017 10,032 3 View