Hello all,
I want to collect Arabidopsis leaves samples exposed to different stress conditions, at different time points. I want to know instead of going for liq. nitrogen can i store my samples in RNAlater. As usage of RNAlater is majorly mentioned for animal and bacterial tissues.
There is no reason why you couldn't use RNA later with plant material. We use RNA later and freeze the samples to get the best results
Dear Pinky Dhatterwal,
Please see the attached paper. It should be useful for you.
From Abstract:
• Methods and Results: Tissues from mosses, cycads, and angiosperm floral organs and leaves were preserved in RNAlater or frozen fresh at −80°C. Extractions were performed and quality was measured for yield and purity.
• Conclusions: This protocol results in the extraction of high-quality RNA from a variety of plant tissues representing vascular and nonvascular plants. RNA was used for cDNA synthesis to generate libraries for next-generation sequencing and for expression studies using quantitative PCR (qPCR) and semiquantitative reverse transcription PCR (RT-PCR).
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