I am trying to extract RNA from the pseudo bulb part of a medicinal terrestrial orchid. although I got high quality RNA from leaf but while trying so from the pseudoibulb or rhizome I am facing huge issues. I have tried qiagen extraction kit and other modified CTAB based extraction modules. However I failed to extract good quality RNA for NGS. It will be of great help if you can please suggest any effective protocol which can eliminate this shortfall.
I have got RNA from bud of poplar tree and I was using plant mini kit qiagen. The most important part was handling the samples!!!
We used to use liquid nitrogen and amount of sample were very important when isolating RNA. Some times one bud was very low to get it!!!
It was very easy to get RNA from leaves, but not easy from reproduction organs. I think that the amount of cell is low in targeted tissues that makes it harder. I would still stick on the qiagen kit.
I hope it helps and good luck...
Ali
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