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Questions related to RNA Extraction
Practical approaches or solutions (strategies) that sports clubs can adopt. A focus on sustainable revenue – that is, income that ensures the club’s financial continuity in the long term, rather...
10 June 2025 1,584 0 View
Hi all, I'm trying to screen the expression level of each isoform with a single probe pair as these isoforms share a lot of identical sequences. Has anyone tried this type of RNA-FISH (HCR)?...
09 June 2025 6,668 0 View
Hi all! I am wondering what factor should be considered when designing split HCR (v3.0) probes and how to design and order them from IDT. I know that it would be most convenient to just get them...
19 May 2025 9,088 0 View
Hi! We are trying to set up an EMSA assay with RNA and an RNA-binding protein. Initially we tested various RNA concentrations without protein to determine what concentration to use moving forward,...
24 April 2025 4,934 3 View
Which is better normal filter Neuclease free water or DEPC treated water for molecular and what’s the basic difference , does DEPC interfere in any run??
04 February 2025 4,054 0 View
The question is about hypothesis testing and its use in analysis for various disciplines, including mathematics, psychology, medicine, and engineering.
20 January 2025 5,597 6 View
I am currently extracting DNA from plasma samples of HBV-positive clinical cases using the QIAGEN Mini Kit (250), following the manufacturer’s protocol step by step. Despite adhering strictly to...
18 January 2025 6,479 0 View
I am exploring the use of ammonia derivatives as passivation layers for perovskite layers in perovskite solar cells (PSCs). Specifically, I would like to understand the relative effectiveness of...
17 January 2025 1,418 0 View
Dear researchgate community, I noticed some abnormalities in my recent bacteria growth curves. The strain is DH5a, growing under inducer of heavy protein expression. Even with this stress,...
30 December 2024 1,848 4 View
Hello, I seem to be running into issues with my brain section in-situ hybridization. There is no signal present and there appears to be some sort of yellow crystaline deposites from the NBT/BCIP...
04 December 2024 6,312 0 View
Good afternoon, I am currently studying Master in Educational Management and pursuing a research about School Administrators' Instructional and Leadership Skills and Teachers' Performance and...
16 November 2024 4,765 6 View
as a co-author or corresponding author
20 October 2024 854 14 View
What is Happy School Model?
20 October 2024 2,983 3 View
in terms of food products in today's globalization trends do students in the university or colleges prefer locally produced brands or foreign produced brands
16 October 2024 3,373 0 View
Integration of all gender and inclusion is prevalent in our society today. Educational management is encompassing and essential to accommodate this rising issue.
05 October 2024 8,781 5 View
Hello all, I am currently doing Dot Blots to confirm that my antibody is present after an Immunoprecipitant run and for some reason my signal is coming through in the FT and Wash but not in the...
03 October 2024 2,624 5 View
What are the Impact of Strategic Human Resource Management on Organisational performance :The mediating role of firm employee relationship.
26 September 2024 2,237 2 View
STRUCTURES OF HR THAT SUPPORTS SUSTAINABLE COMPETITIVE ADVANTAGE
20 September 2024 4,760 4 View
Please explain in the context of curriculum approaches
08 August 2024 2,309 6 View
Hello, I am looking for an online course or document that can help me master MassLynx software. I want to learn how to use it effectively and discover all of its features and tricks.
11 July 2024 2,013 0 View
I see some papers mentioning cutoff values in mg/ml or pg/ml etc. from ROC curves using youden index. How to get the cutoff in values rather than from 0 to 1. I plotted ROC curve in R. Can someone...
22 April 2024 1,711 0 View
Hi guys, I was wondering if it is okay to filter complete StemFlex medium or if I'll lose important factors! Many thanks!
22 April 2024 8,530 1 View
My paper (cancer stem cells the need for a correct definition) was withdrawn from Juniper Publishers (no functional DOI, no indexing)and submitted to a new journal. It must be withdrawn from...
02 April 2024 7,300 1 View
Hi all, My gene causes the cancer cells to grow very slowly when it is overexpressed. Is there a method to make the cells move faster?
30 March 2024 6,087 1 View