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Questions related to RNA Extraction
My paper (cancer stem cells the need for a correct definition) was withdrawn from Juniper Publishers (no functional DOI, no indexing)and submitted to a new journal. It must be withdrawn from...
02 April 2024 7,260 1 View
Hi all, My gene causes the cancer cells to grow very slowly when it is overexpressed. Is there a method to make the cells move faster?
30 March 2024 6,030 1 View
My Phd is in HR area. I want to study the impact of independent variable on dependent variable. And study whether there is significant positive relationship between IV & DV. in such...
25 March 2024 1,942 4 View
PAHs can indicate coal combustion, but how can they more accurately indicate that it is coal combustion?
27 February 2024 9,461 2 View
I will also need the interpretation concerning the reference variable.
31 December 2023 9,618 2 View
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20 December 2023 7,214 9 View
Fish feeding
16 December 2023 8,856 5 View
I am working on 16s rRNA gene sequencing. So for that I should do PCR with random primers. Suggest me with a primer that I should use, also the which universal buffer the ti can use and if...
15 December 2023 6,297 0 View
Which organism is most likely to be found living in the rhizosphere and significance of microorganism present in the rhizosphere and Rhizoplane?
09 December 2023 9,542 4 View
How does PISA influence the formulation of curriculum policies?
06 December 2023 4,492 5 View
What is flipped TRIZ-STEM pedagogy?
05 December 2023 5,467 5 View
Hi everyone. In our lab we genotype samples for our experiments. Apparently we see a band/bands in our control neg ( MQ water). we have changed everything, the buffers, water, primers,...
20 October 2023 1,682 6 View
Hi, While we are doing genotyping of mice, we observed non-specific band which has main band size. So we had 3 negative controls : (1) Taq only (2) Taq + primer (3) Taq +gDNA Surprisingly, we...
16 October 2023 7,738 3 View
Hello, I am trying to use CellProfiler to measure the size and shape of my cells (imaged on brightfield inverted microscope, stained with crystal violet). I use the "IdentifyPrimaryObjects"...
30 September 2023 7,283 1 View
Hello everyone I’m a research assistant who do molecular lab I have experience in western blot many times but the problem is about formula for preparing tris buffer for prepared SDS gel.Because...
11 September 2023 4,148 1 View
Hi all, Does lumen changes in a light box or transilluminator uesd for plaque assay would have great impact on enumeration? Or maybe there is a opportune range of lumen for these equippments when...
30 August 2023 3,334 0 View
The methods which i had already tried from bovine frozen semen. 1. RNAeasy Plus mini kit 2. Trizol method 3. Combination : Trizol + Kit + Beta mercaptoethanol 4. Combination : Somatic cell lysis...
21 July 2023 2,564 1 View
Planning on analysing microbiome from skin biopsies using QIAGEN Microbiome kit. Can't seem to find the best way to homogenise/lyse samples before proceeding with the manufacturer's...
04 May 2023 2,985 1 View
how to calculate IC50 when you have two different concentrations combined? For example : 0.1 µg/L and 0.5µg/L .. etc
10 March 2023 3,331 2 View
Can someone provide me with the authentic methodology to prepare a mold for the formulation of microneedles in the lab? I am struggling to get the mold from a supplier. Although if someone knows...
27 February 2023 1,524 0 View
Hi, I want to do a proliferation assay using HDBECs, but I am unsure on how many cells I should plate at. I want to do this in a 12-well. I bought these from Promocell, and they recommended I do...
31 January 2023 9,171 0 View
I want to fabricate the microneedle. At the first, I should to print the master mold. Then, I cast the PDMS over the master mold to produce negative mold. At the end, polymer-drug solution is cast...
10 November 2022 8,867 2 View
Hi, all! I have generated a biom file in qiime 2, which I am trying to export to R, with the phyloseq package, yet without success. What I have tried is: biom = system.file("extdata",...
15 September 2022 5,447 0 View
Hi all, I have 16s sequences from the V3-V4 region. I was wondering if it is correct to use a neighbor joining tree to calculate unifrac distances, instead of a maximum likelihood tree. Any help...
15 September 2022 5,327 1 View