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Questions related to RNA Extraction
Hi all, I'm trying to screen the expression level of each isoform with a single probe pair as these isoforms share a lot of identical sequences. Has anyone tried this type of RNA-FISH (HCR)?...
09 June 2025 6,638 0 View
Hi all! I am wondering what factor should be considered when designing split HCR (v3.0) probes and how to design and order them from IDT. I know that it would be most convenient to just get them...
19 May 2025 9,062 0 View
Hi! We are trying to set up an EMSA assay with RNA and an RNA-binding protein. Initially we tested various RNA concentrations without protein to determine what concentration to use moving forward,...
24 April 2025 4,885 3 View
Hello, I seem to be running into issues with my brain section in-situ hybridization. There is no signal present and there appears to be some sort of yellow crystaline deposites from the NBT/BCIP...
04 December 2024 6,279 0 View
Good afternoon, I am currently studying Master in Educational Management and pursuing a research about School Administrators' Instructional and Leadership Skills and Teachers' Performance and...
16 November 2024 4,745 6 View
Please explain in the context of curriculum approaches
08 August 2024 2,283 6 View
Hello, I am looking for an online course or document that can help me master MassLynx software. I want to learn how to use it effectively and discover all of its features and tricks.
11 July 2024 1,983 0 View
I see some papers mentioning cutoff values in mg/ml or pg/ml etc. from ROC curves using youden index. How to get the cutoff in values rather than from 0 to 1. I plotted ROC curve in R. Can someone...
22 April 2024 1,670 0 View
Hi guys, I was wondering if it is okay to filter complete StemFlex medium or if I'll lose important factors! Many thanks!
22 April 2024 8,499 1 View
My paper (cancer stem cells the need for a correct definition) was withdrawn from Juniper Publishers (no functional DOI, no indexing)and submitted to a new journal. It must be withdrawn from...
02 April 2024 7,273 1 View
Hi all, My gene causes the cancer cells to grow very slowly when it is overexpressed. Is there a method to make the cells move faster?
30 March 2024 6,045 1 View
My Phd is in HR area. I want to study the impact of independent variable on dependent variable. And study whether there is significant positive relationship between IV & DV. in such...
25 March 2024 1,963 4 View
PAHs can indicate coal combustion, but how can they more accurately indicate that it is coal combustion?
27 February 2024 9,482 2 View
I will also need the interpretation concerning the reference variable.
31 December 2023 9,638 2 View
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20 December 2023 7,228 9 View
Fish feeding
16 December 2023 8,881 5 View
I am working on 16s rRNA gene sequencing. So for that I should do PCR with random primers. Suggest me with a primer that I should use, also the which universal buffer the ti can use and if...
15 December 2023 6,315 0 View
Which organism is most likely to be found living in the rhizosphere and significance of microorganism present in the rhizosphere and Rhizoplane?
09 December 2023 9,559 4 View
How does PISA influence the formulation of curriculum policies?
06 December 2023 4,519 5 View
What is flipped TRIZ-STEM pedagogy?
05 December 2023 5,482 5 View
Hi everyone. In our lab we genotype samples for our experiments. Apparently we see a band/bands in our control neg ( MQ water). we have changed everything, the buffers, water, primers,...
20 October 2023 1,699 6 View
Hi, While we are doing genotyping of mice, we observed non-specific band which has main band size. So we had 3 negative controls : (1) Taq only (2) Taq + primer (3) Taq +gDNA Surprisingly, we...
16 October 2023 7,753 3 View
Hello, I am trying to use CellProfiler to measure the size and shape of my cells (imaged on brightfield inverted microscope, stained with crystal violet). I use the "IdentifyPrimaryObjects"...
30 September 2023 7,302 1 View
Hi Catie I enjoyed your presentation during the resting state brain connectivity conference. Would it be possible to receive a copy of the material presented. Could you shared it online or send it...
21 September 2023 5,475 0 View