Sharing neutrophil isolation and RNA extraction protocol/work-flow?

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No title

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Megha Dubey

Hi Kathryn,

I used dextran sedimentation followed by Percoll gradient centrifugation to isolate neutrophil from human blood and then immediately lysed in Trizol and kept at -20 overnight. You can use EDTA or TSC as anticoagulant both worked fine.

You can check this paper

Article L-Plastin S-glutathionylation promotes reduced binding to β-...

All the best.

Hassan Nima

Good question

Regards