How efficient is the 'SPLIT RNA Extraction Kit (lexogen) for RNA isolation?

More ZHILIAN HU's questions See All

Can anyone provide me a protocl of Native PAGE with the 30% terminal resolving gel concentraion?

I am separating the AuCNs, which are Au nanoclusters. I used the 3% stacking and 30% resolving polyacrylamide from the reference. Eachtime when the resolving gel is polymerizing, plenty of heat...

22 January 2021 8,522 3 View

Naming the "UK coronavirus variant" rises to the occasion?

In the context of the COVID-19 infodemic, the global profusion of monikers and hashtags for "UK coronavirus variant" have found their way into daily communication and contributed to a backlash...

01 January 2021 7,850 2 View

Number of Estimating Equations Required to Test Hypotheses?

After literature review, current draft as below: Variables: Independent = B (represented by I, M, O, S, C), Moderating = W Dependent = F Control = CV Hypotheses: H1a. I has significant...

18 September 2020 7,055 2 View

Why is my protein a smear when I detect with CoIP lysis buffer plus LDS?

Hi I am trying to express my protein R in tobacco leaves and do a CoIP, but I can't detect the protein R in CoIP input. I took the same tissue and added 8M Urea plus LDS protein buffer, protein R...

31 August 2020 9,534 10 View

What's the main difference between #Tris-glycine #SDS-PAGE and #Bis-Tris #SDS-PAGE. How pH and ion strenght affect SDS-PAGE?

sometimes, there is some smear on the SDS-PAGE, this can be eliminated with a different running buffer or a different PAGE, or a different way of sample loading buffer. how these affect the SDS-PAGE?

09 August 2020 6,149 3 View

Is there a gentle method to reduce cuprous oxide to copper while maintaining the original morphology?

Hello, Recently, I have synthesized some cuprous oxide nanomaterials and want to reduce them to metal while maintaining the morphology. Is there a way to do this?

04 August 2020 4,850 3 View

Is there a function coded by matlab to reproject the geographic raster data?

Is there a function coded by matlab to reproject the geographic raster data?especially for the case converting the Ease-Grid 2.0 to the geographic coordinate.

17 June 2020 7,875 2 View

Why the contaminated colony grow around the plate?

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18 May 2020 1,311 3 View

How to do a two-way ONOVA in SPSS?

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30 March 2020 8,769 2 View

Statistical tests for dummies - one way or two way onova?

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24 March 2020 4,220 6 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

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03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

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03 March 2021 8,890 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

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28 February 2021 4,949 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

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28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

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27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

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27 February 2021 5,028 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

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27 February 2021 9,233 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Hassan Nima

this link is useful

https://www.lexogen.com/split-rna-extraction/

regards

Mushtak T. S. Al-Ouqaili

Its ok, but you still have another good options!

Regards.

ZHILIAN HU

Hi, Mushtak,

Do you have any data for this kit? What is another good option? It would be super nice if you could share me the data you got by the way you said. Thanks.