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Questions related to RNA Extraction
Hi all, are LB broth and Nutrient broth minimal or complex media? I am following the QIAGEN protocol for the RNA extraction from bacteria and the protocol for the preparation of lysates is...
02 November 2016 6,221 3 View
I am using RNeasy plus mini kit for extraction of RNA from E. coli. I am following the procedure as written but when I run my eluted sample on the agarose gel to see the RNA integrity (which...
01 November 2016 5,524 7 View
I am using laser capture microdissection to remove a few cells (Between 300-700) per tube, and I was wondering how I can condition a silica column to get good yields of RNA from the lysate? I have...
31 October 2016 7,292 5 View
Hi, so there's my problem. I have done a RNA extraction, ran a gel with it and dosed it. The picture of the gel is after my message and for all my samples, the concentration was between 1250-1550...
28 October 2016 4,527 4 View
I have isolated RNA from keratinocyte cells using Trireagent from SigmaAldrich. With varying concentration, low 260/230, and a spectra that matches phenol contamination, I suspect.......
20 October 2016 6,990 2 View
Now i´m working in RNA isolation of the half part or all the maxilar palate mucosa of BALB/c mouse, with the aim to measure the gene expression of different cytokines with RT-qPCR. But my problem...
11 October 2016 6,348 5 View
Dear Scientists & Researchers, I am going to amplify a specific gene from a (-)ssRNA virus. I have collected several infected tissues and extracted their Total RNA with RNX-Plus solution. Then...
10 October 2016 5,425 3 View
RNA extraction from low volume of blood samples...
10 October 2016 9,366 3 View
In practice I want to do a Mixed Lymphocyte Colture and after incubation I want to isolate only the MSC to extract their RNA. The problem is that MSC are plastic adherent, but also the monocytes...
07 October 2016 4,706 1 View
I am dealing with Candida RNA isolation from TRI reagent (used qiazen kit for extraction).I am really concerned about the quality of my RNA. According to Nanodrop reading my yields were ~100 to...
04 October 2016 8,288 3 View
Is their any protocol or reagent by which I can store mice colon tumor samples at -20 or 2-4℃ and latter extract intact RNA and protein.
03 October 2016 1,843 3 View
I am trying to find out the protocol to extract RNA from the caecum of the chicken. Thinking logically, the condition of the caecum (or even any parts of the intestine) provides a great source of...
03 October 2016 5,925 1 View
Hello, we are using anti CD45+TER119 magnetic beads in order to get a clean endothelial cells fraction. we tried various protocols for RNA isolation, and we think that there might be a problem...
26 September 2016 7,472 4 View
I've tried RNA extraction using ambion kit and trizol with rotor stator homogenizer or liquid nitrogen but i keep getting very low yield (less than 10 ng/uL). starting material 15-25 mg
24 September 2016 6,025 3 View
Hello, I'm new to RNA extraction and RT-qPCR. At the moment, I'm doing a lot of RNA extraction. My first initial result was actually good with high concentrations of RNA and pure. This stops when...
20 September 2016 9,328 2 View
Hello, I'm planning to extract RNA from a sample containing infectious bronchitis virus (gammacoronavirus) purified using a continuous sucrose gradient. Which one of the RNeasy extraction...
20 September 2016 437 1 View
I have to do extract the total RNA from buffalo salivary samples. i would like to have high level gene expression by qPCR..which method is use for getting more quantity of RNA from saliva samples?
16 September 2016 9,172 3 View
I'm trying to preserve mice liver tissue in RNAlater for 1 day and later on to extract total RNA. Has anyone observed RNA degradation after using RNAlater solution? Thank you very much in advance
13 September 2016 8,695 9 View
The concentration and purity of the total RNA was very was very good from Nanodrop1000 measurement out put.
09 September 2016 1,322 3 View
We have LifeGuard solution that is past its expiry date, but has no visual indications of being expired (no precipitates, not cloudy, etc). Has anyone attempted RNA extractions using expired...
09 September 2016 1,735 1 View
I'm doing RNA extraction with TRIZOL, but I just ran out. I saw some bottles identical to the TRIZOL so I assumed I had more but it turned out to be "buffer saturated phenol". is this the same as...
05 September 2016 4,508 3 View
I need to optimize my extraction procedure of RNA from single cell after patch-clamp recording. As I understood the first stage where i can measure the amount of extracted material - Nanodrop...
05 September 2016 1,574 6 View
I need to extract mRNA from my cells but I am not sure if Trizol, a corrosive product, is compatible with the culture 12-well plates. May I should trypsinize and transfer the cells to a eppendorf...
31 August 2016 2,013 4 View
Bacterial pellet with very little medium was stored at 4 degrees.
31 August 2016 4,302 6 View