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Questions related to RNA Extraction
I have gotten a low RNA amount from human dendritic cells using RNeasy kit from Qiagen, such as 11ng/ul and I need to perform a qPCR assay from these samples. Or trizol still is the better way to...
22 February 2017 1,106 3 View
I used Qiagen mini kit for isolation of RNA from mouse bone marrow cells and spleen tissue. The samples were in stored 4 days before at -80C. The 260/280 ratio in 4 different samples is 1.532,...
21 February 2017 9,441 2 View
Has anyone isolated tumor RNA from cell lines for use in cell culture? In all the papers I've read I can see no mention of isolating the RNA in any more sterile manner than is required for any RNA...
21 February 2017 8,710 3 View
i want to extract RNA from the cornea. kindly suggest me a simple method for frozen cornea.
19 February 2017 6,552 5 View
Hello everybody. I'm doing DNA extractions from rizobacteria. The problem is the 260/230 ratio. I have both low and high ratios (eg. 0.76 or 3-4.09). When I search information in Thermo Scientific...
16 February 2017 5,409 5 View
To guarantee good results and purified yield
14 February 2017 774 4 View
I am trying to precipitate RNA isolated from Silkworm larval midgut using absolute and ethanol 3M NaOAc (for storage). The protocol for this has been previously standardized in the lab. It...
11 February 2017 1,561 2 View
Hey everyone! I need some advice for the determination of the mutant adult zebrafish I generated (besides DNA Sequencing). I want to use Western blot to check if the protein is really gone. I...
10 February 2017 237 1 View
I would like to keep my RNA and cDNA samples (from blood and chicken tissues) for long time, Could anybody please suggest me about how and how long for their sample preservation? Many thanks in...
08 February 2017 3,964 5 View
Dear fellow scientist, For RNA isolation I use Trizol, however is pH of Trizol between 4- 6 which allow DNA to be retained in the organic phase and interface, leaving the RNA in the aqueous...
26 January 2017 8,364 6 View
I am preparing to do a maxi-prep and have a pellet bacterial cells that have been at -20 for about 2 months.
05 January 2017 2,341 3 View
Hi all, I extracted RNA from bacteria and I run an agarose gel on these samples. How do I recognize if I have RNA and not DNA? I have three bands (in attachment) and I am not sure about what they...
22 December 2016 8,068 13 View
I am trying to perform trizol RNA extraction on mouse skin samples. After addition of chloroform for phase separation, the top aqueous layer remained very pink - I think from Trizol contamination....
21 December 2016 5,367 5 View
I have already tried different methods to extract RNA from yeast polysomal fractions, like phenol extraction-LiCl precipitation, or total RNA extraction kits (silica columns). In all cases I find...
15 December 2016 6,832 8 View
Hi, I needed to prepare water saturated phenol for RNA extraction. I did as following: liquefy crystal phenol at 60oC, then add 100ml of liquefied phenol to beaker and followed by adding equal...
15 December 2016 1,767 3 View
Hello Everyone, I am trying to extract RNA from Chlamydomonas (Cw92) using the traditional Trizol method. My RNA yield comes up really good around 900 to 1000 ng/ul. Even the 260/280 and 260/230...
14 December 2016 5,656 2 View
I can't find an article with a rough cell count of vaginal lavages so the Trizol datasheet isn't very helpful either. Any ideas?
13 December 2016 6,174 1 View
i am currently doing rna extraction by using trizol reagent. the concentration of rna that i got were around 120-160 ng/µl. The problem that i am having right now is the rna purity (A260/280) is...
12 December 2016 8,602 3 View
Presently, we are trying to isolation miRNA from oocytes. But miRNeasy is not working for isolation of miRNA from a pool of 25 oocytes. Why it is not working with us because we are using low...
09 December 2016 6,589 3 View
Hi all, I am trying to amplify naked mole rat CD44 from CDNA. Sequence of CD44 is taken from http://www.naked-mole-rat.org/annotations/details/XM_004852041.1/. I want to amplify CD44 cDNA. The...
09 December 2016 5,181 4 View
09 December 2016 5,189 5 View
Hi every one! the mortar and pestle that used for cancer tissue grinding (homogenization) to isolate RNA can use for plant grinding and vice versa. and whats types of washing precaution take if we...
03 December 2016 4,993 3 View
Hi, I wanna ask about DNA and RNA pellet. I got the thin white spot at the bottom of the microtube. I assumed that this white spot "pellet" is a DNA pellet or a RNA pellet (ofc after I did DNA or...
02 December 2016 7,671 8 View
I am currently extracting RNA from whole wheat grains at different developmental stages using a CTAB extraction buffer containing: 2% CTAB, 2% PVP, 100mM Tris-Hcl , 25mM EDTA, 2M NaCL and 0.5g...
28 November 2016 1,827 4 View