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Questions related to RNA Extraction
We have infected mosquitoes silenced for a specific protein with Zika virus . After a number of days we dissect the midgut, make a homogenate and try to check for virus replication by plaque...
11 June 2019 8,173 7 View
I want to kno the reason why i got high value for 260/289 ratio and 260/230 ratio
07 June 2019 5,790 6 View
Dear all I have tried 1ml Trizol to extract total RNA from crabs gills. The weight of most of my samples is + -0,03 g. We have applied this protocol for hepatopancreas samples and the results...
04 June 2019 1,604 8 View
I've been trying to extract rna from this particular yeast strain By611 and I could only get one band out of it. I am planning to run q-pcr after this step. Below I am attaching the picture of my...
05 April 2019 1,756 4 View
I'd like to understand better the physiological mechanisms involved in coralliceous encrusting algae grouth ( mainly Lithophyllum spp., Mesophyllum spp., Peyssonnelia spp.) in order to verify if...
07 March 2019 3,064 2 View
we use trazol kit for extrcting RNA from sputum ;although we change our extraction kit we faced low 260/280 ratio of nanodrop.(1.5) according to well-known contamination of sputum...
30 January 2019 855 8 View
Hello everyone, this question has confused me for a while. I am trying to extract RNA from mice brain for RNA sequencing. However, I just noticed that the original protocol I followed recommend...
23 January 2019 5,963 2 View
Hy Everybody, I'm going mad with sealing HT-22 cells in patch clamp experiments. Cells were seaded in DMEM with 10% FBS, 1x Glutamax and penstrep on polyLlysine coated coverslips. - I've...
18 January 2019 6,773 2 View
I want to extract total RNA from pichia pastoris to evaluate the yeast transcriptome using RNA-Seq.
16 January 2019 9,837 1 View
Protocol content (NEB DNAse I reaction Protocol) : RNA: 10ug DNAse reaction buffer 1X 10ul DNAse (Rnase free) 1ul Nuclease free water 100 ul My question is; if I use this protocol after isolating...
12 January 2019 1,553 3 View
I am currently working on the meriofauna (63 - 2000 microns) on the calcareous algae Corallina officinalis in west Wales. I am at some sites turning up numerous spirorbids. This is a group new to...
09 January 2019 9,831 0 View
It is required for the analysis of trophic levels of fishes using their food items
08 January 2019 2,814 5 View
I am using stepone PLus machine and in excel sheet attached to my plate . i found effiency mentioned 1? what does this mean? iam quiet new to the device
06 January 2019 2,882 2 View
RNA extraction from laser microdissected FFPE tissues is markedly enhanced by sonication (paper below). However, I could only find a kit and protocol for Covaris sonicators. We only have a...
21 December 2018 3,120 1 View
I had to perform RNA extraction from the mice skin sample (shaved and hair removed tissue sample of about 1*1 cm square). For that I pulverized the tissue in liquid nitrogen pre-chilled mortar and...
21 December 2018 961 3 View
iam extracting RNA from brain tissues that are stored in -80 .should i get them all at same time from -80 which starting to homogenize some of them on ice or get few sames out of the -80 everynow...
23 November 2018 8,058 1 View
Hi All, I was wondering if anyone had recommendations , insights, comments, etc. on what they think is the best RNA extraction protocol for small tissues. By small, I mean the brain of a tadpole...
31 October 2018 8,111 4 View
I tried to extract plant virus (RNA genome) from ginger leaves but there are a lot of inhibitors when I come to cDNA and RT-PCR sometimes. I want to get expertise advice from those who worked with...
21 October 2018 7,563 4 View
I'm using the RNAzol for extracting total RNA from Gram-positive bacteria that have been harvested in the mid-exponential growth phase. However, we observed bacteria growth is extremely slow for...
18 October 2018 8,949 6 View
We are culturing cells inside a 3D PLGA electrospun scaffold. Currently, we are using Qiazol to isolate the RNA, and we found that the Qiazol dissolves the PLGA scaffold upon contact. And we also...
08 October 2018 1,466 4 View
Hi, I have a question about the 0.5 McFarland standard. In different websites I read that the for the 0.5 McFarland standard, the absorbance at a wavelength of 625 nm should be 0.08 to 0.13. I...
21 September 2018 5,751 1 View
Globally Malaria,dengue and chikungunya are now present where it was not in the past. Is this by climatic factors or others.
12 September 2018 5,643 18 View
I'll work with fecal samples and I want do a good storage for a good RNA extractions in other occasion, I want to know the ratio of fecal samples and RNAlater for a good storage.
11 September 2018 4,580 1 View
I'm trying to figure out how to create a dummy variable in STATA that is dependent on many variables in my dataset. Specifically, I want to create a new binary variable (CMC; 1= yes; 0 =no) where...
20 August 2018 7,740 0 View