Hi,
I am having issues loading both the classifier model and rules from Cell profiler analyst into cell profiler in the filter object module.
I created a pipeline where I identify my primary objects(cells) then I measure object intensity distribution, object intensity, object size and shape and I also measure the texture. I then export to a database and use classifier to train CPA into dividing my objects into 5 cell types.
I then go back to the same pipeline and added an extra module to filter objects as the last module.
And I if select: Objects to filter: Primary objects filtering mode: rules. class number and ask to relabel the additional objects to the 5 different cell types.
But I get the red cross on this module and it says: "The rules file, uses the measurement, Texture_InverseDIfferenceMoment_Clls_3_02 for object IdentifyPrimaryObjects, but that measurement is not available at this stage of the pipeline. Consider editing the rules or adding measurement modules.
It is exactly the same pipeline I use to create the first database with the properties file just with the filter objects module right before the export to database.
If I attempt by selecting classifier instead of rules as filtering mode and upload the model from CPA it gives me “unable to load the model as a classifier file”. But is sees the correct class names underneath.
Can anyone please help me?
Thanks!!!!
Hello Giulia Grimaldi
I did the exact steps as you for my analysis and I am getting the similar error in Filter Objects module even though I used the same pipeline to do the classifiers on CPA. Did you manage to figure out the issue?
Could you please tell me how you solved this problem?
Kind regards,
Vinidhra
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