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Questions related to RNA Extraction
I'm planning to extract the RNA from my bacterial cultures , but the reagents for the Reverse Transcriptase haven't arrived yet. How long could I keep the RNA frozen?
19 April 2017 2,845 6 View
Hello everyone. I have some problems with a RNA extraction. I used the trizol reagent to extract RNA from tissues of leaves and roots under drought stress. When I dissolve the RNA in water, RNA...
16 April 2017 9,672 3 View
I want to check expression of certain genes of bacteria. But bacterial preparation with different treatment is taking long time. This is why i want to store bacterial pellet after centrifugation...
14 April 2017 3,276 3 View
Dear all, is there anyone which experienced to homogenate tissues in trizol, but did not proceed immediately with RNA extraction? I mean, is it possible to homogenate in trizol and store your...
13 April 2017 6,419 3 View
Hi all, is there any protocol to purify high quality RNA from rodent samples? I checked quite few articles, but the RNA quality does not meet the highly pure criteria.
10 April 2017 1,817 2 View
I extracted RNA from my cells cultured in soft agar culture system by TRIZOL, but unfortunately pollution in RNA yield is high so I couldn't detect genes expression by real time PCR. Any one has...
08 April 2017 1,815 3 View
Hi everyone! Fungi total RNA was extracted by mirVana miRNA isolation kit using total RNA extraction protocol, but the RNA profile shows a large portion of small RNA, which seems unlike a...
07 April 2017 2,399 4 View
I have been isolating RNA from CD14 monocytes purified from PBMCs but the yield has been low. I've read that DNase treatment can result in lower RNA yields, but I want to ensure that these results...
06 April 2017 4,276 4 View
We are trying to detect gene amplification in canine tissue sections (histomorphology is important). The amplified region is about 60 kb in length. What is the best way to prepare the probe,...
04 April 2017 9,008 2 View
Dear researchers, I have attempted to do a gene expression study. The first step was isolate the RNA. I have checked the RNA yield using Nanodrop, which had the following nucleic Acid...
31 March 2017 8,119 3 View
I am trying to isolate a mRNA (1400 bases) from Dog placenta with trizol reagent. Every time we are getting degraded RNA. We have stored the 100mg sample in 300ul trizol reagent and stored at -80....
30 March 2017 1,414 2 View
I am trying to isolate DNA from the conidia of fungus using SDS. The conidia is directly isolated from plant (not cultured) and seems to have tough cell wall and somehow i managed to crush to...
30 March 2017 6,782 3 View
I use QuiaCube automated RNA extraction from PB MNCs??
27 March 2017 9,826 4 View
I am working on stool samples stored under glycerin , but there is an excess and mixing of glycerin with the stool sample , this lead to lowering the RNA yield due to sample dilution, How can i...
15 March 2017 1,358 1 View
Hi there, I am extracting RNA from environmental samples like soil. I have a three stage protocol. First I use a Chloroform/Phenol based extraction according to Toewe et all, then I am using a...
14 March 2017 907 7 View
I'm extracting total RNA from yeast with MasterPure- Yeast RNA Purification Kit - Epicentre but when I run the sample in a gel I can see plasmidic DNA left even after treatment with DNAse I. Is...
10 March 2017 7,290 3 View
I'm using the Ambion RNAqueous Micro Kit to extract RNA from cells in different pathways. I use laser capture microdissection to extract the cells. From one pathway, I normally collect 8-50 cells....
08 March 2017 8,670 4 View
Hi all, in our lab we would like to switch from Mercaptoethanol to TCEP as a reducing agent for RNA protection during isolation. What concentration do you use? I only found a protocoll from...
02 March 2017 4,638 2 View
Hi, Is there a method to extract RNA of both gram positive and gram negative bacteria in their dual-species culture? I want to perform transcriptomic analysis, so I need RNA sample with high...
02 March 2017 8,132 2 View
Can anyone help me how to increase RNA yield from exosomes ? I was able to get RNA concentrations of only around 30-40ng/ul per sample from my exosomes and I would be happy to hear any suggestions...
28 February 2017 7,750 7 View
RNA extraction
28 February 2017 5,989 2 View
I have RNA samples stored at -70º, that have been extracted from animal blood, excrements, urine and saliva about 3 years ago. I wonder if this RNA samples are stable to use in a RT-PCR assay.
27 February 2017 3,899 6 View
HI. I isolated RNA many times, and suddenly something weird happened: I used TRI-reagent 0.5 ml, and stored the tubes on the tubes rotator for 10 min. I added 50 uL BCP, waited 5 min, and...
23 February 2017 2,381 5 View
Hello, What's the best way to disrupt a tissue without affecting too much to the RNA? I will use RNeasy for RNA extraction, but before I have to homogenize my sample, and I think using a needle...
23 February 2017 1,110 3 View