It is said that root sampling is a critical task to be done since it would influence the quality of your samples. I would like to ask for a root sampling procedure in rice which takes in to consideration the rhizosphere microbiome, which later I will use the samples for RNA extraction and RT-PCR. I have little information wherein phosphate buffered saline (PBS) solution and liquid N will be used but the said information is incomplete, thus I need a detailed procedure to carry out my experiment.

Thank you for your response!